Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	972,866	Δ1 bp	coding (536/780 nt)	elyC ←	envelope biogenesis factor

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	972,863	0	G	.	100.0%	86.5 / NA	21	coding (539/780 nt)	elyC	envelope biogenesis factor
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base . (9/12);  total (9/12)

AGATCATCGCCCGCGGCAGATGTGATGCGGAGGTCACCAGCAGGAAAGGGGCGTCACCAATCGCCTGTTTCACTGCTGCAGCTTCTTCTTC  >  NC_000913/972816‑972906                                                |                                            aGATCATCGCCCGCGGCAGATGTGATGCGGAGGTCACCAGCAGGAAAggg                                           >  1:1358824/1‑50 (MQ=255)      atcGCCCGCGGCAGATGTGATGCGGAGGTCACCAGCAGGAAA‑GGGCGTCa                                     >  1:291002/1‑50 (MQ=39)      atcGCCCGCGGCAGATGTGATGCGGAGGTCACCAGCAGGAAA‑GGGCGTCa                                     >  1:2044568/1‑50 (MQ=39)         gCCCGCGGCAGATGTGATGCGGAGGTCACCAGCAGGAAA‑GGGCGTCAcc                                   <  1:284970/49‑1 (MQ=39)         gCCCGCGGCAGATGTGATGCGGAGGTCACCAGCAGGAAA‑GGGCGTCAcc                                   >  1:1326514/1‑49 (MQ=39)           ccGCGGCAGATGTGATGCGGAGGTCACCAGCAGGAAA‑GGGCGTCACCAAt                                <  1:1695348/50‑1 (MQ=39)            cgcgGCAGATGTGATGCGGAGGTCACCAGCAGGAAA‑GGGCGTCACCAATc                               <  1:954661/50‑1 (MQ=39)            cgcgGCAGATGTGATGCGGAGGTCACCAGCAGGAAA‑GGGCGTCACCAATc                               <  1:1608280/50‑1 (MQ=39)             gcgGCAGATGTGATGCGGAGGTCACCAGCAGGAAA‑GGGCGTCACCAATCg                              >  1:1171876/1‑50 (MQ=39)             gcgGCAGATGTGATGCGGAGGTCACCAGCAGGAAA‑GGGCGTCACCAATCg                              >  1:383711/1‑50 (MQ=39)                gCAGATGTGATGCGGAGGTCACCAGCAGGAAA‑GGGCGTCACCAATCGCCt                           <  1:576109/50‑1 (MQ=39)                gCAGATGTGATGCGGAGGTCACCAGCAGGAAA‑GGGCGTCACCAATCGCCt                           <  1:790301/50‑1 (MQ=39)                            cGGAGGTCACCAGCAGGAAA‑GGGCGTCACCAATCGCCTGTTTCActgct                <  1:311090/49‑1 (MQ=39)                              gAGGTCACCAGTAGGAAA‑GGGCGTCACCAATCGCCTGTTTCActgctg               >  1:874654/1‑48 (MQ=38)                                ggTCACCAGCAGGAAA‑GGGCGTCACCAATCGCCTGTTTCACTGCTGCAGc           <  1:738003/50‑1 (MQ=39)                                 gTCACCAGCAGGAAA‑GGGCGTCACCAATCGCCTGTTTCACTGCTGCAGct          >  1:1045267/1‑50 (MQ=39)                                 gTCACCAGCAGGAAA‑GGGCGTCACCAATCGCCTGTTTCACTGCTGCAGct          <  1:1018244/50‑1 (MQ=39)                                   cACCAGCAGGAAA‑GGGCGTCACCAATCGCCTGTTTCACTGCTGCAGcttc        >  1:1969967/1‑50 (MQ=39)                                    aCCAGCAGGAAA‑GGGCGTCACCAATCGCCTGTTTCACTGCTGCAGcttc        <  1:1799129/49‑1 (MQ=39)                                      cagcagGAAA‑GGGCGTCACCAATCGCCTGTTTCACTGCTGCAGcttcttc     <  1:1443534/50‑1 (MQ=39)                                       agcagGAAA‑GGGCGTCACCAATCGCCTGTTTCACTGCTGCAGcttcttct    >  1:2032556/1‑50 (MQ=39)                                         cagGAAA‑GGGCGTCACCAATCGCCTGTTTCACTGCTGCAGCttcttcttc  <  1:2141548/50‑1 (MQ=39)                                                |                                            AGATCATCGCCCGCGGCAGATGTGATGCGGAGGTCACCAGCAGGAAAGGGGCGTCACCAATCGCCTGTTTCACTGCTGCAGCTTCTTCTTC  >  NC_000913/972816‑972906

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑