| Predicted mutation | ||||||
|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | annotation | gene | description |
| RA | NC_000913 | 3,948,805 | (G)6→7 | coding (1165/1521 nt) | yifB ← | putative magnesium chelatase YifB |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NC_000913 | 3,948,799 | 1 | . | G | 96.0% | 88.7 / ‑3.6 | 25 | coding (1171/1521 nt) | yifB | putative magnesium chelatase YifB |
| Reads supporting (aligned to +/- strand): ref base . (0/1); new base G (16/8); total (16/9) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 3.60e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00 | |||||||||||
ACGGTGGCGCTGCTTTCTCCCGGCACTACCGTTTTACTCAAAAT‑GCC‑GGGGGGTGGTAATGGGATCTCCAGTGAGAGATCGAAGCGGTC > NC_000913/3948753‑3948841 | aCGGTGGCGCTGCTTTCTCCCGGCACTACCGTTTTACTCAAAAT‑GCC‑gg < 1:2057402/49‑1 (MQ=255) tGGCGCTGCTTTCTCCCGGCACTACCGTTTTACTCAAAAT‑GCC‑ggggggg > 1:1415922/1‑49 (MQ=255) gctTTCTCCCGGCACTACCGTTTTACTCAAAAT‑GCCGGGGGGGTGGTAAt > 1:2096752/1‑50 (MQ=255) ctTTCTCCCGGCACTACCGTTTTACTCAAAAT‑GCCGGGGGGGTGGTAATg > 1:1145281/1‑50 (MQ=255) tttCTCCCGGCACTACCGTTTTACTCAAAAT‑GCCGGGGGGGTGGTAATgg > 1:2065914/1‑50 (MQ=255) ttCTCCCGGCACTACCGTTTTACTCAAAAT‑GCCGGGGGGGTGGTAATgg > 1:1410447/1‑49 (MQ=255) tctcCCGGCACTACCGTTTTACTCAAAAT‑GCCGGGGGGGTGGTAATgg < 1:1768356/48‑1 (MQ=255) ctcCCGGCACTACCGTTTTACTCAAAAT‑GCCGGGGGGGTGGTAATGGGa < 1:2022795/49‑1 (MQ=255) ctcCCGGCACTACCGTTTTACTCAAAAT‑GCCGGGGGGGTGGTAATGGGAt > 1:131041/1‑50 (MQ=255) tcCCGGCACTACCGTTTTACTCAAAAT‑GCCGGGGGGGTGGTAATGGGAtc > 1:214749/1‑50 (MQ=255) ccGGCACTACCGTTTTACTCAAAAT‑GCCGGGGGGGTGGTAATGGGAt < 1:1345795/47‑1 (MQ=255) ggCACTACCGTTTTACTCAAAAT‑GCCGGGGGGGTGGTAATGGGAtctc > 1:1825280/1‑48 (MQ=255) cACTACCGTTTTACTCAAAAT‑GCCGGGGGGGTGGTAATGGGATCTCCAGt < 1:2210878/50‑1 (MQ=255) cACTACCGTTTTACTCAAAAT‑GCCGGGGGGGTGGTAATGGGATCTCCAGt < 1:262926/50‑1 (MQ=255) cACTACCGTTTTACTCAAAAT‑GCCGGGGGGGTGGTAATGGGATCTCCAGt > 1:363354/1‑50 (MQ=255) cTACCGTTTTACTCAAAAT‑GCCGGGGGGGTGGTAATGGGATCTCCAGt > 1:1408360/1‑48 (MQ=255) tACCGTTTTACTCAAAAT‑GCCGGGGGGGTGGTAATGGGATCTCCAGt > 1:1389190/1‑47 (MQ=255) ggTTTTAC‑CAAAATGGCG‑GGGGGGTGGTAATGGGATCTCCAGTGAGAGAt < 1:812365/49‑1 (MQ=255) gTTTTACTCAAAAT‑GCCGGGGGGGTGGTAATGGGATCTCCAGTGAGAGAt < 1:1118359/50‑1 (MQ=255) ttttACTCAAAAT‑GCCGGGGGGGTGGTAATGGGATCTCCAGTga > 1:1237768/1‑44 (MQ=255) ttttACTCAAAAT‑GCCGGGGGGGTGGTAATGGGATCTCCAGTga > 1:610047/1‑44 (MQ=255) ttttACTCAAAAT‑GCCGGGGGGGTGGTAATGGGATCTCCAGTGAGAGATc < 1:1964099/50‑1 (MQ=255) tCAAAAT‑GCCGGGGGGGTGGTAATGGGATCTCCAGTGAGAGATCGAAGCg > 1:1096033/1‑50 (MQ=255) aaaaT‑GCCGGGGGGGTGGTAATGGGATCTCCAGTGAGAGATCGAAGCGGt > 1:459819/1‑50 (MQ=255) aaaaT‑GCCGGGGGGGTGGTAATGGGATCTCCAGTGAGAGATCGAAGCGGt < 1:104330/50‑1 (MQ=255) aaT‑GCCGGGGGGGTGGTAATGGGATCTCCAGTGAGAGATCGAAGCGGTc > 1:710826/1‑49 (MQ=255) aT‑GCCGGGGGGGTGGTAATGGGATCTCCAGTGAGAGATCGAAGCGGTc > 1:1742856/1‑48 (MQ=255) | ACGGTGGCGCTGCTTTCTCCCGGCACTACCGTTTTACTCAAAAT‑GCC‑GGGGGGTGGTAATGGGATCTCCAGTGAGAGATCGAAGCGGTC > NC_000913/3948753‑3948841 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 31 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |