Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	12,086	C→T	intergenic (‑300/‑77)	yaaI ← / → dnaK	DUF2541 domain‑containing protein YaaI/chaperone protein DnaK

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	12,086	0	C	T	100.0%	90.4 / NA	25	intergenic (‑300/‑77)	yaaI/dnaK	DUF2541 domain‑containing protein YaaI/chaperone protein DnaK
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base T (14/11);  total (14/11)

GTAGTCAACCGCAGTGAGTGAGTCTGCAAAAAAATGAAATTGGGCAGTTGAAACCAGACGTTTCGCCCCTATTACAGACTCACAACCACAT  >  NC_000913/12042‑12132                                             |                                               gtagtCAACCGCAGTGAGTGAGTCTGCAAAAAAATGAAATTGGGTAGtt                                            <  1:2196053/49‑1 (MQ=255) gtagtCAACCGCAGTGAGTGAGTCTGCAAAAAAATGAAATTGGGTAGtt                                            <  1:2089931/49‑1 (MQ=255)  tagtCAACCGCAGTGAGTGAGTCTGCAAAAAAATGAAATTGGGTAGTTGa                                          <  1:1574661/50‑1 (MQ=255)       aaCCGCAGTGAGTGAGTCTGCAAAAAAATGAAATTGGGTAGTTGAAAcc                                      <  1:745586/49‑1 (MQ=255)           gCAGTGAGTGAGTCCGCAAAAAAATGAAATTGGGTAGTTGAAACCAGACg                                 >  1:1628694/1‑50 (MQ=39)             agtgagtgAGTCTGCAAAAAAATGCAATTGGGTAGTTGAAACCAGACGtt                               >  1:1984972/1‑50 (MQ=255)             agtgagtgAGTCTGCAAAAAAATGAAATTGGGTAGTTGAAACCAGACGtt                               >  1:690164/1‑50 (MQ=255)               tgagtgagTCTGCAAAAAAATGAAATTGGGTAGTTGAAACCAGACGTTTc                             >  1:423635/1‑50 (MQ=255)                gagtgagtCTGCAAAAAAATGAAATTGGGTAGTTGAAACCAGACGTTTCg                            >  1:1234147/1‑50 (MQ=255)                gagtgagtCTGCAAAAAAATGAAATTGGGTAGTTGAAACCAGACGTTTCg                            >  1:1124991/1‑50 (MQ=255)                     agtCTGCAAAAAAATGAAATTGGGTAGTTGAAACCAGACGTTTCGcccc                        >  1:2141055/1‑49 (MQ=255)                       tCTGCAAAAAAATGAAATTGGGTAGTTGAAACCAGACGTTTCGCCCCTAt                     <  1:726710/50‑1 (MQ=255)                         tGCAAAAAAATGAAATTGGGTAGTTGAAACCAGACGTTTCGCCCCTATTa                   >  1:1134802/1‑50 (MQ=255)                          gCAAAAAAATGAAATTGGGTAGTTGAAACCAGACGTTTCGCCCCTATTa                   >  1:1727886/1‑49 (MQ=255)                           cAAAAAAATGAAATTGGGTAGTTGAAACCAGACGTTTCGCCCCTATTa                   <  1:647756/48‑1 (MQ=255)                           cAAAAAAATGAAATTGGGTAGTTGAAACCAGACGTTTCGCCCCTATTAc                  <  1:1253308/49‑1 (MQ=255)                           cAAAAAAATGAAATTGGGTAGTTGAAACCAGACGTTTCGCCCCTATTACa                 <  1:391592/50‑1 (MQ=255)                            aaaaaaaTGAAATTGGGTAGTTGAAACCAGACGTTTCGCCCCTATTACAg                <  1:866450/50‑1 (MQ=255)                                  aTGAAATTGGGTAGTTGAAACCAGACGTTTCGCCCCTATTACAGACTca           >  1:1110555/1‑49 (MQ=255)                                   tGAAATTGGGTAGTTGAAACCAGACGTTTCGCCCCTATTACAGACTcaca         <  1:1516016/50‑1 (MQ=255)                                   tGAAATTGGGTAGTTGAAACCAGACGTTTCGCCCCTATTACAGACTcac          >  1:1827318/1‑49 (MQ=255)                                    gAAATTGGGTAGTTGAAACCAGACGTTTCGCCCCTATTACAGACTCACaa        >  1:1202307/1‑50 (MQ=255)                                      aaTTGGGTAGTTGAAACCAGACGTTTCGCCCCTATTACAGACTCACAAcc      <  1:1801395/50‑1 (MQ=255)                                         tGGGTAGTTGAAACCAGACGTTTCGCCCCTATTACAGACTCACAACcaca   >  1:1506021/1‑50 (MQ=255)                                          gggTAGTTGAAACCAGACGTTTCGCCCCTATTACAGACTCACAACCACat  >  1:1692732/1‑50 (MQ=255)                                             |                                               GTAGTCAACCGCAGTGAGTGAGTCTGCAAAAAAATGAAATTGGGCAGTTGAAACCAGACGTTTCGCCCCTATTACAGACTCACAACCACAT  >  NC_000913/12042‑12132

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 16 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑