Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	4,093,223	A→G	V72V (GTT→GTC)	rhaM ←	L‑rhamnose mutarotase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	4,093,223	0	A	G	100.0%	106.3 / NA	28	V72V (GTT→GTC)	rhaM	L‑rhamnose mutarotase
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base G (12/16);  total (12/16)

CGGGCATAACATCGGTCATATATTTCCACCAACGTTGGCAAACATCAGTGCTGGCAACCGCATTCCAGCGTTCTTCAGATTCAATCT  >  NC_000913/4093183‑4093269                                         |                                               cGGGCATAACATCGGTCATATATTTCCACCAACGTTGGCAGACATCAGTg                                       >  1:1117169/1‑50 (MQ=255) cGGGCATAACATCGGTCATATATTTCCACCAACGTTGGCAGACATCAGTg                                       >  1:157789/1‑50 (MQ=255) cGGGCATAACATCGGTCATATATTTCCACCAACGTTGGCAGACATCAGTg                                       <  1:911725/50‑1 (MQ=255)   ggCATAACATCGGTCATATATTTCCACCAACGTTGGCAGACATCAGTGCt                                     <  1:1975901/50‑1 (MQ=255)     cATAACATCGGTCATATATTTCCACCAACGTTGGCAGACATCAGTGCTgg                                   >  1:1432725/1‑50 (MQ=255)      aTAACATCGGTCATATATTTCCACCAACGTTGGCAGACATCAGTGCTGGc                                  <  1:1624551/50‑1 (MQ=255)      aTAACATCGGTCATATATTTCCACCAACGTTGGCAGACATCAGTGCTGGc                                  <  1:1959354/50‑1 (MQ=255)           aTCGGTCATATATTTCCACCAACGTTGGCAGACATCAGTGCTGGCAACCg                             >  1:542228/1‑50 (MQ=255)           aTCGGTCATATATTTCCACCAACGTTGGCAGACATCAGTGCTGGCAACCg                             <  1:1436915/50‑1 (MQ=255)            tCGGTCATATATTTCCACCAACGTTGGCAGACATCAGTGCTGGCAACCg                             >  1:2065479/1‑49 (MQ=255)            tCGGTCATATATTTCCACCAACGTTGGCAGACATCAGTGCTGGCAACCGc                            <  1:1450172/50‑1 (MQ=255)              ggTCATATATTTCCACCAACGTTGGCAGACATCAGTGCTGGCAACCGCAt                          <  1:2119183/50‑1 (MQ=255)               gTCATATATTTCCACCAACGTTGGCAGACATCAGTGCTGGCAACCGCAtt                         <  1:686453/50‑1 (MQ=255)                 cATATATTTCCACCAACGTTGGCAGACATCAGTGCTGGCAACCGCATTc                        >  1:702659/1‑49 (MQ=255)                   tatatTTCCACCAACGTTGGCAGACATCAGTGCTGGCAACCGCATTCCAg                     <  1:585556/50‑1 (MQ=255)                    atatTTCCACCAACGTTGGCAGACATCAGTGCTGGCAACCGCATTCCAGc                    >  1:1762129/1‑50 (MQ=255)                       tttCCACCAACGTTGGCAGACATCAGTGCTGGCAACCGCATTCCAGCGtt                 <  1:972612/50‑1 (MQ=255)                       tttCCACCAACGTTGGCAGACATCAGTGCTGGCAACCGCATTCCAGCGt                  <  1:604109/49‑1 (MQ=255)                         tCCACCAACGTTGGCAGACATCAGTGCTGGCAACCGCATTCCAGCGttc                <  1:1579486/49‑1 (MQ=255)                          ccaccaACGTTGGCAGACATCAGTGCTGGCAACCGCATTCCAGCGttctt              >  1:976659/1‑50 (MQ=255)                            accaACGTTGGCAGACATCAGTGCTGGCAACCGCATTCCAGCGttctt              >  1:1307955/1‑48 (MQ=255)                            accaACGTTGGCAGACATCAGTGCTGGCAACCGCATTCCAGCGTTCTTCa            <  1:836615/50‑1 (MQ=255)                               aaCGTTGGCAGACATCAGTGCTGGCAACCGCATTCCAGCGTTCTTCAGAt         >  1:2044534/1‑50 (MQ=255)                                aCGTTGGCAGACATCAGTGCTGGCAACCGCATTCCAGCGTTCTTCAGAtt        >  1:1731920/1‑50 (MQ=255)                                  gTTGGCAGACATCAGTGCTGGCAACCGCATTCCAGCGTTCTTCAGATTc       >  1:2204486/1‑49 (MQ=255)                                     ggCAGACATCAGTGCTGGCAACCGCATTCCAGCGTTCTTCAGATTCAAt    <  1:632031/49‑1 (MQ=255)                                      gCAGACATCAGTGCTGGCAACCGCATTCCAGCGTTCTTCAGATTCAAtct  <  1:109458/50‑1 (MQ=255)                                      gCAGACATCAGTGCTGGCAACCGCATTCCAGCGTTCTTCAGATTCAAtc   <  1:1710258/49‑1 (MQ=255)                                         |                                               CGGGCATAACATCGGTCATATATTTCCACCAACGTTGGCAAACATCAGTGCTGGCAACCGCATTCCAGCGTTCTTCAGATTCAATCT  >  NC_000913/4093183‑4093269

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑