Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	4,485,906	C→A	A16S (GCC→TCC)	pepA ←	aminopeptidase A/I

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	4,485,906	0	C	A	100.0%	102.3 / NA	26	A16S (GCC→TCC)	pepA	aminopeptidase A/I
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base A (12/14);  total (12/14)

AATCGGAGAAAGGCGACGTGGTTCGAAGACGCCCACGACGATGCAGGCACTCCGCTGTTTCTCCGGGCTACCGCTTTTTACACTAAACTCCATG  >  NC_000913/4485859‑4485952                                                |                                               aaTCGGAGAAAGGCGACGTGGTTCGAAGACGCCCACGACGATGCAGGAAc                                              >  1:568241/1‑50 (MQ=255)   tCGGAGAAAGGCGACGTGGTTCGAAGACGCCCACGACGATGCAGGAACTc                                            >  1:1714549/1‑50 (MQ=255)    cGGAGAAAGGCGACGTGGTTCGAAGACGCCCACGACGATGCAGGAACTcc                                           >  1:5595/1‑50 (MQ=255)        gaAAGGCGACGTGGTTCGAAGACGCCCACGACGATGCAGGAACTCCGCTg                                       >  1:1196242/1‑50 (MQ=255)          aaGGCGACGTGGTTCGAAGACGCCCACGACGATGCAGGAACTCCGCTGtt                                     <  1:2005734/50‑1 (MQ=255)            ggCGACGTGGTTCGAAGACGCCCACGACGATGCAGGAACTCCGCTGTTtc                                   >  1:1981184/1‑50 (MQ=255)             gCGACGTGGTTCGAAGACGCCCACGACGATGCAGGAACTCCGCTGTTtct                                  <  1:1810085/50‑1 (MQ=255)               gACGTGGTTCGAAGACGCCCACGACGATGCAGGAACTCCGCTGTTTCTcc                                >  1:769978/1‑50 (MQ=255)               gACGTGGTTCGAAGACGCCCACGACGATGCAGGAACTCCGCTGTTTCTcc                                <  1:714632/50‑1 (MQ=255)                aCGTGGTTCGAAGACGCCCACGACGATGCAGGAACTCCGCTGTTTCTCCg                               <  1:1604017/50‑1 (MQ=255)                 cGTGGTTCGAAGACGCCCACGACGATGCAGGAACTCCGCTGTTTCTCCgg                              <  1:1573312/50‑1 (MQ=255)                     gTTCGAAGACGCCCACGACGATGCAGGAACTCCGCTGTTTCTCCGGGCt                           <  1:657607/49‑1 (MQ=255)                        cGAAGACGCCCACGACGATGCAGGAACTCCGCTGTTTCTCCGGGCTAcc                        <  1:1635700/49‑1 (MQ=255)                        cGAAGACGCCCACGACGATGCAGGAACTCCGCTGTTTCTCCGGGCTACCg                       >  1:1006933/1‑50 (MQ=255)                          aaGACGCCCACGACGATGCAGGAACTCCGCTGTTTCTCCGGGCTACCGCt                     >  1:1209715/1‑50 (MQ=255)                            gACGCCCACGACGATGCAGGAACTCCGCTGTTTCTCCGGGCTACCGCttt                   >  1:1214226/1‑50 (MQ=255)                               gCCCACGACGATGCAGGAACTCCGCTGTTTCTCCGGGCTACCGCTTTTTa                >  1:1849363/1‑50 (MQ=255)                                cccACGACGATGCAGGAACTCCGCTGTTTCTCCGGGCTACCGCTTTTTac               <  1:950917/50‑1 (MQ=255)                                  cACGACGATGCAGGAACTCCGCTGTTTCTCCGGGCTACCGCTTTTTacac             <  1:2122966/50‑1 (MQ=255)                                   acgacgATGCAGGAACTCCGCTGTTTCTCCGGGCTACCGCTTTTTacac             >  1:458885/1‑49 (MQ=255)                                    cgacgaTGCAGGAACTCCGCTGTTTCTCCGGGCTACCGCTTTTTACACTa           <  1:24108/50‑1 (MQ=255)                                     gacgaTGCAGGAACTCCGCTGTTTCTCCGGGCTACCGCTTTTTACACTaa          <  1:2240193/50‑1 (MQ=255)                                       cgaTGCAGGAACTCCGCTGTTTCTCCGGGCTACCGCTTTTTACACTAAAc        <  1:562510/50‑1 (MQ=255)                                       cgaTGCAGGAACTCCGCTGTTTCTCCGGGCTACCGCTTTTTACACTAAAc        >  1:1805032/1‑50 (MQ=255)                                            cAGGAACTCCGCTGTTTCTCCGGGCTACCGCTTTTTACACTAAACGCCAt   <  1:1859062/50‑1 (MQ=255)                                             aGGAACTCCGCTGTTTCTCCGGGCTACCGCTTTTTACACTAAACTCCATg  <  1:59618/50‑1 (MQ=255)                                                |                                               AATCGGAGAAAGGCGACGTGGTTCGAAGACGCCCACGACGATGCAGGCACTCCGCTGTTTCTCCGGGCTACCGCTTTTTACACTAAACTCCATG  >  NC_000913/4485859‑4485952

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑