Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	988,499	G→A	P163S (CCA→TCA)	asnS ←	asparagine‑‑tRNA ligase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	988,499	0	G	A	100.0%	92.2 / NA	24	P163S (CCA→TCA)	asnS	asparagine‑‑tRNA ligase
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base A (12/12);  total (12/12)

GCGGAACATTTCGCCTGCACCTTCGGTATCAGATGCGGTAATCAGTGGCGTTGAAACCCAGAAGAATCCCTGCTCGTTAAAGAAGCGATGCAG  >  NC_000913/988452‑988544                                                |                                              gcgGAACATTTCGCCTGCACCTTCGGTATCAGATGCGGTAATCAGTGACg                                             <  1:776495/50‑1 (MQ=255)   ggAACATTTCGCCTGCACCTTCGGTATCAGATGCGGTAATCAGTGACGtt                                           <  1:1176853/50‑1 (MQ=255)   ggAACATTTCGCCTGCACCTTCGGTATCAGATGCGGTAATCAGTGACGtt                                           >  1:1602326/1‑50 (MQ=255)      aCATTTCGCCTGCACCTTCGGTATCAGATGCGGTAATCAGTGACGTTGaa                                        <  1:702914/50‑1 (MQ=255)        aTTTCGCCTGCACCTTCGGTATCAGATGCGGTAATCAGTGACGTTGAAAc                                      >  1:228932/1‑50 (MQ=255)           tCGCCTGCACCTTCGGTATCAGATGCGGTAATCAGTGACGTTGAAAccc                                    <  1:1268440/49‑1 (MQ=255)             gCCTGCACCTTCGGTATCAGATGCGGTAATCAGTGACGTTGAAACCCag                                  <  1:1237364/49‑1 (MQ=255)              ccTGCACCTTCGGTATCAGATGCGGTAATCAGTGACGTTGAAACCCagaa                                <  1:2182351/50‑1 (MQ=255)               cTGCACCTTCGGTATCAGATGCGGTAATCAGTGACGTTGAAACCCagaag                               <  1:194109/50‑1 (MQ=255)                tGCACCTTCGGTATCAGATGCGGTAATCAGTGACGTTGAAACCCagaag                               >  1:1380962/1‑49 (MQ=255)                 gCACCTTCGGTATCAGATGCGGTAATCAGTGACGTTGAAACCCAgaagaa                             >  1:885642/1‑50 (MQ=255)                    ccTTCGGTATCAGATGCGGTAATCAGTGACGTTGAAACCCAGAAGAATcc                          >  1:1833646/1‑50 (MQ=255)                    ccTTCGGTATCAGATGCGGTAATCAGTGACGTTGAAACCCAGAAGAATcc                          <  1:433586/50‑1 (MQ=255)                      ttCGGTATCAGATGCGGTAATCAGTGACGTTGAAACCCAGAAGAATCCCt                        >  1:1698160/1‑50 (MQ=255)                         ggTATCAGATGCGGTAATCAGTGACGTTGAAACCCAGAAGAATCCCTGCt                     <  1:473045/50‑1 (MQ=255)                          gTATCAGATGCGGTAATCAGTGACGTTGAAACCCAGAAGAATCCCTGCTc                    >  1:1494240/1‑50 (MQ=255)                             tCAGATGCGGTAATCAGTGACGTTGAAACCCAGAAGAATCCCTGCTCGt                  <  1:1116194/49‑1 (MQ=255)                              cAGATGCGGTAATCAGTGACGTTGAAACCCAGAAGAATCCCTGCTCGtt                 >  1:2108913/1‑49 (MQ=255)                                 aTGCGGTAATCAGTGACGTTGAAACCCAGAAGAATCCCTGCTCGTTa                >  1:195735/1‑47 (MQ=255)                                    cGGTAATCAGTGACGTTGAAACCCAGAAGAATCCCTGCTCGTTAaagaa           <  1:620820/49‑1 (MQ=255)                                    cGGTAATCAGTGACGTTGAAACCCAGAAGAATCCCTGCTCGTTAaagaa           <  1:66700/49‑1 (MQ=255)                                          tCAGTGACGTTGAAACCCAGAAGAATCCCTGCTCGTTAAAGAAGCGATg     >  1:2172509/1‑49 (MQ=255)                                           cAGTGACGTTGAAACCCAGAAGAATCCCTGCTCGTTAAAGAAGCGATg     >  1:1697386/1‑48 (MQ=255)                                            aGTGACGTTGAAACCCAGAAGAATCCCTGCTCGTTAAAGAAGCGATGCAg  >  1:885797/1‑50 (MQ=255)                                                |                                              GCGGAACATTTCGCCTGCACCTTCGGTATCAGATGCGGTAATCAGTGGCGTTGAAACCCAGAAGAATCCCTGCTCGTTAAAGAAGCGATGCAG  >  NC_000913/988452‑988544

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 18 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑