Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	1,154,362	C→T	A84V (GCC→GTC)	mltG →	endolytic murein transglycosylase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	1,154,362	0	C	T	100.0%	119.1 / NA	30	A84V (GCC→GTC)	mltG	endolytic murein transglycosylase
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base T (17/13);  total (17/13)

TTTTCAATGGCTGCTGCGTATCGAACCGGATCTTTCTCACTTTAAAGCCGGGACTTACCGCTTTACACCGCAGATGACCGTGCGCGAGATGC  >  NC_000913/1154315‑1154406                                                |                                             ttttCAATGGCTGCTGCGTATCGAACCGGATCTTTCTCACTTTAAAGTCg                                            >  1:471393/1‑50 (MQ=255)  tttCAATGGCTGCTGCGTATCGAACCGGATCTTTCTCACTTTAAAGTCgg                                           >  1:2182024/1‑50 (MQ=255)  tttCAATGGCTGCTGCGTATCGAACCGGATCTTTCTCACTTTAAAGTCg                                            <  1:518238/49‑1 (MQ=255)    tCAATGGCTGCTGCGTATCGAACCGGATCTTTCTCACTTTAAAGTCGGGa                                         >  1:744974/1‑50 (MQ=255)     cAATGGCTGCTGCGTATCGAACCGGATCTTTCTCACTTTAAAGTCGGGAc                                        >  1:1019926/1‑50 (MQ=255)      aaTGGCTGCTGCGTATCGAACCGGATCTTTCTCACTTTAAAGTCGGGAc                                        <  1:1917136/49‑1 (MQ=255)      aaTGGCTGCTGCGTATCGAACCGGATCTTTCTCACTTTAAAGTCGGGACt                                       >  1:1455546/1‑50 (MQ=255)          gctgctGCGTATCGAACCGGATCTTTCTCACTTTAAAGTCGGGACTTAcc                                   <  1:1463505/50‑1 (MQ=255)          gctgctGCGTATCGAACCGGATCTTTCTCACTTTAAAGTCGGGACTTAcc                                   <  1:1968451/50‑1 (MQ=255)          gctgctGCGTATCGAACCGGATCTTTCTCACTTTAAAGTCGGGACTTAcc                                   >  1:202011/1‑50 (MQ=255)              ctgcGTATCGAACCGGATCTTTCTCACTTTAAAGTCGGGACTTACCGCtt                               <  1:541153/50‑1 (MQ=255)                 cGTATCGAACCGGATCTTTCTCACTTTAAAGTCGGGACTTACCGCttt                              <  1:2038602/48‑1 (MQ=255)                 cGTATCGAACCGGATCTTTCTCACTTTAAAGTCGGGACTTACCGCttt                              <  1:2174178/48‑1 (MQ=255)                  gTATCGAACCGGATCTTTCTCACTTTAAAGTCGGGACTTACCGCTTTaca                           >  1:1772776/1‑50 (MQ=255)                   tATCGAACCGGATCTTTCTCACTTTAAAGTCGGGACTTACCGCTTTacac                          <  1:641652/50‑1 (MQ=255)                        aaCCGGATCTTTCTCACTTTAAAGTCGGGACTTACCGCTTTACACCGCAg                     <  1:814843/50‑1 (MQ=255)                          ccGGATCTTTCTCACTTTAAAGTCGGGACTTACCGCTTTACACCGCAGAt                   >  1:1465066/1‑50 (MQ=255)                          ccGGATCTTTCTCACTTTAAAGTCGGGACTTACCGCTTTACACCGCAGAt                   <  1:1629124/50‑1 (MQ=255)                           cGGATCTTTCTCACTTTAAAGTCGGGACTTACCGCTTTACACCGCAGAt                   >  1:444943/1‑49 (MQ=255)                           cGGATCTTTCTCACTTTAAAGTCGGGACTTACCGCTTTACACCGCAGATg                  <  1:240457/50‑1 (MQ=255)                              aTCTTTCTCACTTTAAAGTCGGGACTTACCGCTTTACACCGCAGATGAcc               >  1:859648/1‑50 (MQ=255)                                 tttCTCACTTTAAAGTCGGGACTTACCGCTTTACACCGCAGATGACCGTg            >  1:751529/1‑50 (MQ=255)                                  ttCTCACTTTAAAGTCGGGACTTACCGCTTTACACCGCAGATGACCGTgc           >  1:606437/1‑50 (MQ=255)                                  ttCTCACTTTAAAGTCGGGACTTACCGCTTTACACCGCAGATGACCGTgc           >  1:158770/1‑50 (MQ=255)                                   tctcACTTTAAAGTCGGGACTTACCGCTTTACACCGCAGATGACCGTgcg          >  1:482840/1‑50 (MQ=255)                                   tctcACTTTAAAGTCGGGACTTACCGCTTTACACCGCAGATGACCGTgcg          >  1:1201539/1‑50 (MQ=255)                                   tctcACTTTAAAGTCGGGACTTACCGCTTTACACCGCAGATGACCGTg            <  1:1990915/48‑1 (MQ=255)                                      cACTTTAAAGTCGGGACTTACCGCTTTACACCGCAGATGACCGTgcgc         >  1:1566616/1‑48 (MQ=255)                                           tAAAGTCGGGACTTACCGCTTTACACCGCAGATGACCGTGCGCGAGAt    <  1:101097/48‑1 (MQ=255)                                           tAAAGTCGGGACTTACCGCTTTACACCGCAGATGACCGTGCGCGAGATGc  >  1:2172587/1‑50 (MQ=255)                                                |                                             TTTTCAATGGCTGCTGCGTATCGAACCGGATCTTTCTCACTTTAAAGCCGGGACTTACCGCTTTACACCGCAGATGACCGTGCGCGAGATGC  >  NC_000913/1154315‑1154406

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑