Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	1,270,811	C→T	V347M (GTG→ATG)	chaA ←	Na(+)/K(+):H(+) antiporter ChaA

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	1,270,811	0	C	T	100.0%	99.0 / NA	26	V347M (GTG→ATG)	chaA	Na(+)/K(+):H(+) antiporter ChaA
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base T (10/16);  total (10/16)

ATCAAATAGGCGGCAAACAGTGCCAGATGCGCTGCGCCATTGAGCACGTTAGTACGTCCGGTGGAGAAGGAGATATGGCACAGCACTAAAGAG  >  NC_000913/1270765‑1270857                                               |                                               atcaAATAGGCGGCAAACAGTGCCAGATGCGCTGCGCCATTGAGCATGtt                                             <  1:561949/50‑1 (MQ=255) atcaAATAGGCGGCAAACAGTGCCAGATGCGCTGCGCCATTGAGCATGt                                              >  1:2145630/1‑49 (MQ=255) atcaAATAGGCGGCAAACAGTGCCAGATGCGCTGCGCCATTGAGCATGt                                              <  1:1968039/49‑1 (MQ=255)  tcaAATAGGCGGCAAACAGTGCCAGATGCGCTGCGCCATTGAGCATGt                                              >  1:24454/1‑48 (MQ=255)     aaTAGGCGGCAAACAGTGCCAGATGCGCTGCGCCATTGAGCATGTTAGTa                                         <  1:1248876/50‑1 (MQ=255)     aaTAGGCGGCAAACAGTGCCAGATGCGCTGCGCCATTGAGCATGTTAGTa                                         <  1:619307/50‑1 (MQ=255)       tAGGCGGCAAACAGTGCCAGATGCGCTGCGCCATTGAGCATGTTAGTACg                                       >  1:1715860/1‑50 (MQ=255)        aGGCGGCAAACAGTGCCAGATGCGCTGCGCCATTCAGCATGTTAGTACGt                                      >  1:1229015/1‑50 (MQ=39)             gcAAACAGTGCCAGATGCGCTGCGCCATTGAGCATGTTAGTACGTCCGGt                                 >  1:830603/1‑50 (MQ=255)             gcAAACAGTGCCAGATGCGCTGCGCCATTGAGCATGTTAGTACGTCCGGt                                 <  1:2234493/50‑1 (MQ=255)              cAAACAGTGCCAGATGCGCTGCGCCATTGAGCATGTTAGTACGTCCGGTg                                >  1:1328039/1‑50 (MQ=255)              cAAACAGTGCCAGATGCGCTGCGCCATTGAGCATGTTAGTACGTCCGGTg                                <  1:2025213/50‑1 (MQ=255)                       ccAGATGCGCTGCGCCATTGAGCATGTTAGTACGTCCGGTGGAGAAGga                        <  1:36374/49‑1 (MQ=255)                        cAGATGCGCTGCGCCATTGAGCATGTTAGTACGTCCGGTGGAGAAGgaga                      <  1:566255/50‑1 (MQ=255)                         aGATGCGCTGCGCCATTGAGCATGTTAGTACGTCCGGTGGAGAAGgaga                      >  1:954313/1‑49 (MQ=255)                                 tgcgcCATTGAGCATGTTAGTACGTCCGGTGGAGAAGGAGATATGGca               >  1:208837/1‑48 (MQ=255)                                   cgcCATTGAGCATGTTAGTACGTCCGGTGGAGAAGGAGATATGGcaca             <  1:2017613/48‑1 (MQ=255)                                   cgcCATTGAGCATGTTAGTACGTCCGGTGGAGAAGGAGATATGGcaca             <  1:667206/48‑1 (MQ=255)                                   cgcCATTGAGCATGTTAGTACGTCCGGTGGAGAAGGAGATATGGCACAGc           <  1:76490/50‑1 (MQ=255)                                   cgcCATTGAGCATGTTAGTACGTCCGGTGGAGAAGGAGATATGGCACAGc           <  1:826192/50‑1 (MQ=255)                                       aTTGAGCATGTTAGTACGTCCGGTGGAGAAGGAGATATGGCACAGCACTa       <  1:1291460/50‑1 (MQ=255)                                        ttGAGCATGTTAGTACGTCCGGTGGAGAAGGAGATATGGCACAGCACTa       >  1:1373636/1‑49 (MQ=255)                                          gAGCATGTTAGTACGTCCGGTGGAGAAGGAGATATGGCACAGCACTaaa     <  1:1641385/49‑1 (MQ=255)                                          gAGCATGTTAGTACGTCCGGTGGAGAAGGAGATATGGCACAGCACTa       >  1:1608219/1‑47 (MQ=255)                                           aGCATGTTAGTACGTCCGGTGGAGAAGGAGATATGGCACAGCACTAAaga   <  1:1680364/50‑1 (MQ=255)                                            gCATGTTAGTACGTCCGGTGGAGAAGGAGATATGGCACAGCACTAAagag  <  1:24501/50‑1 (MQ=255)                                               |                                               ATCAAATAGGCGGCAAACAGTGCCAGATGCGCTGCGCCATTGAGCACGTTAGTACGTCCGGTGGAGAAGGAGATATGGCACAGCACTAAAGAG  >  NC_000913/1270765‑1270857

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑