Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	1,382,555	C→T	S203S (AGC→AGT)	ompG →	outer membrane porin G

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	1,382,555	0	C	T	100.0%	86.8 / NA	22	S203S (AGC→AGT)	ompG	outer membrane porin G
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base T (7/15);  total (7/15)

CGAGTGAACTATTATCTCGAGCGCGGCTTCAATATGGACGACAGCCGCAATAACGGTGAGTTTTCCACGCAAGAAATTCGCGCCTATTTGC  >  NC_000913/1382511‑1382601                                             |                                               cGAGTGAACTATTATCTCGAGCGCGGCTTCAATATGGACGACAGTCGCaa                                           <  1:88955/50‑1 (MQ=255)  gAGTGAACTATTATCTCGAGCGCGGCTTCAATATGGACGACAGTCGCaa                                           >  1:436642/1‑49 (MQ=255)   aGTGAACTATTATCTCGAGCGCGGCTTCAATATGGACGACAGTCGCAATa                                         <  1:1353231/50‑1 (MQ=255)      gAACTATTATCTCGAGCGCGGCTTCAATATGGACGACAGTCGCAATAACg                                      >  1:1269/1‑50 (MQ=255)       aaCTATTATCTCGAGCGCGGCTTCAATATGGACGACAGTCGCAATAACg                                      <  1:1176525/49‑1 (MQ=255)            ttatCTCGAGCGCGGCTTCAATATGGACGACAGTCGCAATAACGGTGAg                                 <  1:1882811/49‑1 (MQ=255)                  cGAGCGCGGCTTCAATATGGACGACAGTCGCAATAACGGTGAGTTTTCCa                          >  1:459750/1‑50 (MQ=255)                   gAGCGCGGCTTCAATATGGACGACAGTCGCAATAACGGTGAGTTTTc                            <  1:817528/47‑1 (MQ=255)                   gAGCGCGGCTTCAATATGGACGACAGTCGCAATAACGGTGAGTTTTCCAc                         >  1:1767775/1‑50 (MQ=255)                   gAGCGCGGCTTCAATATGGACGACAGTCGCAATAACGGTGAGTTTTCCAc                         >  1:560429/1‑50 (MQ=255)                    aGCGCGGCTTCAATATGGACGACAGTCGCAATAACGGTGAGTTTTCCACg                        <  1:1531017/50‑1 (MQ=255)                        cgGCTTCAATATGGACGACAGTCGCAATAACGGTGAGTTTTCCACGCAAg                    <  1:650090/50‑1 (MQ=255)                        cgGCTTCAATATGGACGACAGTCGCAATAACGGTGAGTTTTCCACGCAAg                    <  1:711796/50‑1 (MQ=255)                         ggCTTCAATATGGACGACAGTCGCAATAACGGTGAGTTTTCCACGCAAGa                   <  1:506514/50‑1 (MQ=255)                               aaTATGGACGACAGTCGCAATAACGGTGAGTTTTCCACGCAAGAAATTcg             >  1:953484/1‑50 (MQ=255)                               aaTATGGACGACAGTCGCAATAACGGTGAGTTTTCCACGCAAGAAATTc              <  1:240947/49‑1 (MQ=255)                               aaTATGGACGACAGTCGCAATAACGGTGAGTTTTCCACGCAAGAAATTc              <  1:1485219/49‑1 (MQ=255)                                   tGGACGACAGTCGCAATAACGGTGAGTTTTCCACGCAAGAAATTCGCGcc         <  1:985037/50‑1 (MQ=255)                                        gacAGTCGCAATAACGGTGAGTTTTCCACGCAAGAAATTCGCGCCTAttt    <  1:1268856/50‑1 (MQ=255)                                        gacAGTCGCAATAACGGTGAGTTTTCCACGCAAGAAATTCGCGCCTAttt    <  1:1575313/50‑1 (MQ=255)                                          cAGTCGCAATAACGGTGAGTTTTCCACGCAAGAAATTCGCGCCTATTTGc  >  1:261966/1‑50 (MQ=255)                                          cAGTCGCAATAACGGTGAGTTTTCCACGCAAGAAATTCGCGCCTATTTGc  <  1:1451688/50‑1 (MQ=255)                                             |                                               CGAGTGAACTATTATCTCGAGCGCGGCTTCAATATGGACGACAGCCGCAATAACGGTGAGTTTTCCACGCAAGAAATTCGCGCCTATTTGC  >  NC_000913/1382511‑1382601

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 18 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑