Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	1,662,872	C→T	R773W (CGG→TGG)	ynfF →	putative selenate reductase YnfF

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	1,662,872	0	C	T	100.0%	77.8 / NA	20	R773W (CGG→TGG)	ynfF	putative selenate reductase YnfF
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base T (8/12);  total (8/12)

GCCATCGGGCAAGGTGCGTGGCTTAAAGCGGATATGTTTGGTGACCGGGTCGATCACGGCGGCAGTATCAATATTCTGACCTCTCACCGCC  >  NC_000913/1662827‑1662917                                              |                                              gCCATCGGGCAAGGTGCGTGGCTTAAAGCGGATATGTTTGGTGACTGGGt                                           <  1:990728/50‑1 (MQ=255)  ccATCGGGCAAGGTGCGTGGCTTAAAGCGGATATGTTTGGTGACTGGGTc                                          <  1:1940159/50‑1 (MQ=255)         gCAAGGTGCGTGGCTTAAAGCGGATATGTTTGGTGACTGGGTCGATCAcg                                   <  1:2018530/50‑1 (MQ=255)           aaGGTGCGTGGCTTAAAGCGGATATGTTTGGTGACTGGGTCGATCAcggc                                 <  1:921464/50‑1 (MQ=255)           aaGGTGCGTGGCTTAAAGCGGATATGTTTGGTGACTGGGTCGATCAcggc                                 >  1:220999/1‑50 (MQ=255)                 cGTGGCTTAAAGCGGATATGTTTGGTGACTGGGTCGATCACGGCGGCAg                            <  1:1531051/49‑1 (MQ=255)                  gTGGCTTAAAGCGGATATGTTTGGTGACTGGGTCGATCACGGCGGCAGTa                          >  1:2084997/1‑50 (MQ=255)                     gCTTAAAGCGGATATGTTTGGTGACTGGGTCGATCACGGCGGCAGTATc                        >  1:1963658/1‑49 (MQ=255)                         aaaGCGGATATGTTTGGTGACTGGGTCGATCACGGCGGCAGTATCAat                     <  1:763202/48‑1 (MQ=255)                          aaGCGGATATGTTTGGTGACTGGGTCGATCACGGCGGCAGTATCAatat                   <  1:646335/49‑1 (MQ=255)                            gCGGATATGTTTGGTGACTGGGTCGATCACGGCGGCAGTATCAATATTCt                >  1:337809/1‑50 (MQ=255)                             cGGATATGTTTGGTGACTGGGTCGATCACGGCGGCAGTATCAATATTCTg               <  1:290332/50‑1 (MQ=255)                                atatGTTTGGTGACTGGGTCGATCACGGCGGCAGTATCAATATTCTGAcc            >  1:1048173/1‑50 (MQ=255)                                  atGTTTGGTGACTGGGTCGATCACGGCGGCAGTATCAATATTCTGACctc          >  1:305069/1‑50 (MQ=255)                                   tGTTTGGTGACTGGGTCGATCACGGCGGCAGTATCAATATTCTGACctct         <  1:1295316/50‑1 (MQ=255)                                     tttGGTGACTGGGTCGATCACGGCGGCAGTATCAATATTCTGACCTCTCa       <  1:1487779/50‑1 (MQ=255)                                        ggTGACTGGGTCGATCACGGCGGCAGTATCAATATTCTGACCTCTCACCg    >  1:1776698/1‑50 (MQ=255)                                         gTGACTGGGTCGATCACGGCGGCAGTATCAATATTCTGACCTCTCACCg    <  1:921367/49‑1 (MQ=255)                                          tGACTGGGTCGATCACGGCGGCAGTATCAATATTCTGACCTCTCACCGcc  >  1:699730/1‑50 (MQ=255)                                          tGACTGGGTCGATCACGGCGGCAGTATCAATATTCTGACCTCTCACCGcc  <  1:898929/50‑1 (MQ=255)                                              |                                              GCCATCGGGCAAGGTGCGTGGCTTAAAGCGGATATGTTTGGTGACCGGGTCGATCACGGCGGCAGTATCAATATTCTGACCTCTCACCGCC  >  NC_000913/1662827‑1662917

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 30 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑