Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	1,742,755	G→C	P163R (CCG→CGG)	ribC ←	riboflavin synthase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	1,742,755	0	G	C	100.0%	122.7 / NA	32	P163R (CCG→CGG)	ribC	riboflavin synthase
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base C (14/18);  total (14/18)

CGCCAAGTTTTTTCTTCCCAAGAGTCGTGCGTTCCAGTGTTTCCGGAATTAAATGGACGCAAAAACGCGTTGGCGTGACTTCGCCGACGG  >  NC_000913/1742711‑1742800                                             |                                              cgcCAAGTTTTTTCTTCCCAAGAGTCGTGCGTTCCAGTGTTTCCCGAAtt                                          <  1:1598416/50‑1 (MQ=255) cgcCAAGTTTTTTCTTCCCAAGAGTCGTGCGTTCCAGTGTTTCCCGAAtt                                          <  1:651654/50‑1 (MQ=255)  gcCAAGTTTTTTCTTCCCAAGAGTCGTGCGTTCCAGTGTTTCCCGAATTa                                         <  1:88673/50‑1 (MQ=255)     aaGTTTTTTCTTCCCAAGAGTCGTGCGTTCCAGTGTTTCCCGAATTAAAt                                      <  1:1500850/50‑1 (MQ=255)        ttttttCTTCCCAAGAGTCGTGCGTTCCAGTGTTTCCCGAATTAAATGGa                                   <  1:414164/50‑1 (MQ=255)          ttttCTTCCCAAGAGTCGTGCGTTCCAGTGTTTCCCGAATTAAATGGAc                                  <  1:1075346/49‑1 (MQ=255)           tttCTTCCCAAGAGTCGTGCGTTCCAGTGTTTCCCGAATTAAATGGACg                                 <  1:990583/49‑1 (MQ=255)           tttCTTCCCAAGAGTCGTGCGTTCCAGTGTTTCCCGAATTAAATGGACg                                 <  1:423209/49‑1 (MQ=255)            ttcttcCCAAGAGTCGTGCGTTCCAGTGTTTCCCGAATTAAATGGACGc                                >  1:1615206/1‑49 (MQ=255)            ttcttcCCAAGAGTCGTGCGTTCCAGTGTTTCCCGAATTAAATGGACGc                                >  1:2075034/1‑49 (MQ=255)              cttcCCAAGAGTCGTGCGTTCCAGTGTTTCCCGAATTAAATGGACGCaa                              <  1:1438999/49‑1 (MQ=255)                 cccAAGAGTCGTGCGTTCCAGTGTTTCCCGAATTAAATGGACGCaaa                             <  1:369036/47‑1 (MQ=255)                   cAAGAGTCGTGCGTTCCAGTGTTTCCCGAATTAAATGGACGCAAAAAcgc                        >  1:2165450/1‑50 (MQ=255)                    aaGAGTCGTGCGTTCCAGTGTTTCCCGAATTAAATGGACGCAAAAAcgcg                       >  1:294604/1‑50 (MQ=255)                      gagTCGTGCGTTCCAGTGTTTCCCGAATTAAATGGACGCAAAAACGCGtt                     <  1:1853968/50‑1 (MQ=255)                       agTCGTGCGTTCCAGTGTTTCCCGAATTAAATGGACGCAAAAACGCGTTg                    <  1:183228/50‑1 (MQ=255)                       agTCGTGCGTTCCAGTGTTTCCCGAATTAAATGGACGCAAAAACGCGTTg                    <  1:801523/50‑1 (MQ=255)                        gTCGTGCGTTCCAGTGTTTCCCGAATTAAATGGACGCAAAAACGCGTTgg                   >  1:594332/1‑50 (MQ=255)                        gTCGTGCGTTCCAGTGTTTCCCGAATTAAATGGACGCAAAAACGCGTTgg                   <  1:279426/50‑1 (MQ=255)                          cGTGCGTTCCAGTGTTTCCCGAATTAAATGGACGCAAAAACGCGTTGGCg                 >  1:1814321/1‑50 (MQ=255)                           gTGCGTTCCAGTGTTTCCCGAATTAAATGGACGCAAAAACGCGTTGGCGt                <  1:505206/50‑1 (MQ=255)                                ttCCAGTGTTTCCCGAATTAAATGGACGCAAAAACGCGTTGGCGTGACtt           <  1:1793566/50‑1 (MQ=255)                                ttCCAGTGTTTCCCGAATTAAATGGACGCAAAAACGCGTTGGCGTGACtt           <  1:597958/50‑1 (MQ=255)                                 tCCAGTGTTTCCCGAATTAAATGGACGCAAAAACGCGTTGGCGTGACTTc          >  1:2028914/1‑50 (MQ=255)                                 tCCAGTGTTTCCCGAATTAAATGGACGCAAAAACGCGTTGGCGTGACTTc          >  1:1176832/1‑50 (MQ=255)                                 tCCAGTGTTTCCCGAATTAAATGGACGCAAAAACGCGTTGGCGTGACTTc          >  1:939356/1‑50 (MQ=255)                                      tgtTTCCCGAATTAAATGGACGCAAAAACGCGTTGGCGTGACTTCGCCGa     >  1:33959/1‑50 (MQ=255)                                      tgtTTCCCGAATTAAATGGACGCAAAAACGCGTTGGCGTGACTTCGCCGa     >  1:1664091/1‑50 (MQ=255)                                       gtTTCCCGAATTAAATGGACGCAAAAACGCGTTGGCGTGACTTCGCCGAc    >  1:1203459/1‑50 (MQ=255)                                       gtTTCCCGAATTAAATGGACGCAAAAACGCGTTGGCGTGACTTCGCCGAc    >  1:1106063/1‑50 (MQ=255)                                         ttCCCGAATTAAATGGACGCAAAAACGCGTTGGCGTGACTTCGCCGACgg  >  1:564044/1‑50 (MQ=255)                                          tCCCGAATTAAATGGACGCAAAAACGCGTTGGCGTGACTTCGCCGACgg  <  1:91956/49‑1 (MQ=255)                                             |                                              CGCCAAGTTTTTTCTTCCCAAGAGTCGTGCGTTCCAGTGTTTCCGGAATTAAATGGACGCAAAAACGCGTTGGCGTGACTTCGCCGACGG  >  NC_000913/1742711‑1742800

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑