Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	1,776,533	G→A	G316D (GGC→GAC)	ydiF →	putative acetate‑CoA transferase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	1,776,533	0	G	A	100.0%	117.9 / NA	31	G316D (GGC→GAC)	ydiF	putative acetate‑CoA transferase
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base A (8/23);  total (8/23)

TAAAGGCGCGGTGGGGAATGTCGGCGTCGGTATTGCTGACGGCATTGGCCTGGTCGCCCGAGAAGAAGGTTGTGCTGATGACTTTATTCTGACG  >  NC_000913/1776486‑1776579                                                |                                               tAAAGGCGCGGTGGGGAATGTCGGCGTCGGTATTGCTGACGGCATTGAcc                                              <  1:633021/50‑1 (MQ=255)  aaaGGCGCGGTGGGGAATGTCGGCGTCGGTATTGCTGACGGCATTGACCt                                             >  1:1595715/1‑50 (MQ=255)    aGGCGCGGTGGGGAATGTCGGCGTCGGTATTGCTGACGGCATTGACCTgg                                           <  1:1184173/50‑1 (MQ=255)    aGGCGCGGTGGGGAATGTCGGCGTCGGTATTGCTGACGGCATTGACCTgg                                           <  1:1564292/50‑1 (MQ=255)      gcgcGGTGGGGAATGTCGGCGTCGGTATTGCTGACGGCATTGACCTGGTc                                         <  1:2193709/50‑1 (MQ=255)          ggTGGGGAATGTCGGCGTCGGTATTGCTGACGGCATTGACCTGGTCGccc                                     >  1:822540/1‑50 (MQ=255)           gTGGGGAATGTCGGCGTCGGTATTGCTGACGGCATTGACCTGGTCGccc                                     >  1:1012224/1‑49 (MQ=255)            tGGGGAATGTCGGCGTCGGTATTGCTGACGGCATTGACCTGGTCGCCCga                                   <  1:1786658/50‑1 (MQ=255)             ggggAATGTCGGCGTCGGTATTGCTGACGGCATTGACCTGGTCGccc                                     >  1:1463551/1‑47 (MQ=255)                 aaTGTCGGCGTCGGTATTGCTGACGGCATTGACCTGGTCGCCCGagaaga                              <  1:1387653/50‑1 (MQ=255)                  aTGTCGGCGTCGGTATTGCTGACGGCATTGACCTGGTCGCCCGAgaagaa                             <  1:154194/50‑1 (MQ=255)                   tGTCGGCGTCGGTATTGCTGACGGCATTGACCTGGTCGCCCGAGaagaag                            <  1:854660/50‑1 (MQ=255)                       ggCGTCGGTATTGCTGACGGCATTGACCTGGTCGCCCGAGAAGAAGGtt                         <  1:1599331/49‑1 (MQ=255)                           tCGGTATTGCTGACGGCATTGACCTGGTCGCCCGAGAAGAAGGTTGTGCt                    <  1:1305184/50‑1 (MQ=255)                             ggTATTGCTGACGGCATTGACCTGGTCGCCCGAGAAGAAGGTTGTGCtg                   <  1:1857444/49‑1 (MQ=255)                             ggTATTGCTGACGGCATTGACCTGGTCGCCCGAGAAGAAGGTTGTGCtg                   <  1:672262/49‑1 (MQ=255)                               tATTGCTGACGGCATTGACCTGGTCGCCCGAGAAGAAGGTTGTGCtgatg                <  1:918395/50‑1 (MQ=255)                                 ttGCTGACGGCATTGACCTGGTCGCCCGAGAAGAAGGTTGTGCtgatga               <  1:1623764/49‑1 (MQ=255)                                 ttGCTGACGGCATTGACCTGGTCGCCCGAGAAGAAGGTTGTGCtgatga               <  1:881837/49‑1 (MQ=255)                                   gCTGACGGCATTGACCTGGTCGCCCGAGAAGAAGGTTGTGCTGATGACtt            >  1:1008082/1‑50 (MQ=255)                                   gCTGACGGCATTGACCTGGTCGCCCGAGAAGAAGGTTGTGCTGATGACtt            >  1:434729/1‑50 (MQ=255)                                   gCTGACGGCATTGACCTGGTCGCCCGAGAAGAAGGTTGTGCTGATGACt             <  1:60722/49‑1 (MQ=255)                                    cTGACGGCATTGACCTGGTCGCCCGAGAAGAAGGTTGTGCTGATGACttt           <  1:644186/50‑1 (MQ=255)                                    cTGACGGCATTGACCTGGTCGCCCGAGAAGAAGGTTGTGCTGATGACtt            >  1:192897/1‑49 (MQ=255)                                       aCGGCATTGACCTGGTCGCCCGAGAAGAAGGTTGTGCTGATGACTTTAtt        <  1:1501935/50‑1 (MQ=255)                                          gCATTGACCTGGTCGCCCGAGAAGAAGGTTGTGCTGATGACTTTATTCTg     <  1:509851/50‑1 (MQ=255)                                            aTTGACCTGGTCGCCCGAGAAGAAGGTTGTGCTGATGACTTTATTCTGAc   <  1:288248/50‑1 (MQ=255)                                            aTTGACCTGGTCGCCCGAGAAGAAGGTTGTGCTGATGACTTTATTCTGAc   <  1:1523415/50‑1 (MQ=255)                                            aTTGACCTGGTCGCCCGAGAAGAAGGTTGTGCTGATGACTTTATTCTGAc   >  1:1406284/1‑50 (MQ=255)                                             ttGACCTGGTCGCCCGAGAAGAAGGTTGTGCTGATGACTTTATTCTGACg  <  1:1882841/50‑1 (MQ=255)                                             ttGACCTGGTCGCCCGAGAAGAAGGTTGTGCTGATGACTTTATTCTGACg  <  1:639430/50‑1 (MQ=255)                                                |                                               TAAAGGCGCGGTGGGGAATGTCGGCGTCGGTATTGCTGACGGCATTGGCCTGGTCGCCCGAGAAGAAGGTTGTGCTGATGACTTTATTCTGACG  >  NC_000913/1776486‑1776579

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑