Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	1,811,208	T→C	V92A (GTT→GCT)	tcyP →	cystine/sulfocysteine:cation symporter

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	1,811,208	0	T	C	100.0%	139.9 / NA	37	V92A (GTT→GCT)	tcyP	cystine/sulfocysteine:cation symporter
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base C (22/15);  total (22/15)

GCAAATGATCGTTATGCCGTTAGTCTTCGCCTCTATTCTGAGCGCGGTTGCCCGTCTGCATAACGCATCTCAGTTAGGCAAAATCAGTTTTCTG  >  NC_000913/1811161‑1811254                                                |                                               gcAAATGATCGTTATGCCGTTAGTCTTCGCCTCTATTCTGAGCGCGGCTg                                              >  1:1123759/1‑50 (MQ=255)  cAAATGATCGTTATGCCGTTAGTCTTCGCCTCTATTCTGAGCGCGGCTGc                                             >  1:83378/1‑50 (MQ=255)   aaaTGATCGTTATGCCGTTAGTCTTCGCCTCTATTCTGAGCGCGGCTGcc                                            >  1:1896634/1‑50 (MQ=255)       gATCGTTATGCCGTTAGTCTTCGCCTCTATTCTGAGCGCGGCTGCCCGTc                                        >  1:1493084/1‑50 (MQ=255)        aTCGTTATGCCGTTAGTCTTCGCCTCTATTCTGAGCGCGGCTGCCCGTCt                                       <  1:1153188/50‑1 (MQ=255)           gTTATGCCGTTAGTCTTCGCCTCTATTCTGAGCGCGGCTGCCCGTCTGCa                                    >  1:889240/1‑50 (MQ=255)            ttATGCCGTTAGTCTTCGCCTCTATTCTGAGCGCGGCTGCCCGTCTGCAt                                   <  1:688805/50‑1 (MQ=255)            ttATGCCGTTAGTCTTCGCCTCTATTCTGAGCGCGGCTGCCCGTCTGCAt                                   <  1:1383396/50‑1 (MQ=255)            ttATGCCGTTAGTCTTCGCCTCTATTCTGAGCGCGGCTGCCCGTCTGCAt                                   <  1:1753220/50‑1 (MQ=255)             tATGCCGTTAGTCTTCGCCTCTATTCTGAGCGCGGCTGCCCGTCTGCAt                                   >  1:1996427/1‑49 (MQ=255)               tGCCGTTAGTCTTCGCCTCTATTCTGAGCGCGGCTGCCCGTCTGCATaa                                 <  1:1272515/49‑1 (MQ=255)               tGCCGTTAGTCTTCGCCTCTATTCTGAGCGCGGCTGCCCGTCTGCATaa                                 <  1:2057890/49‑1 (MQ=255)                gCCGTTAGTCTTCGCCTCTATTCTGAGCGCGGCTGCCCGTCTGCATAACg                               >  1:1558365/1‑50 (MQ=255)                  cGTTAGTCTTCGCCTCTATTCTGAGCGCGGCTGCCCGTCTGCATAACGCa                             >  1:1033787/1‑50 (MQ=255)                   gTTAGTCTTCGCCTCTATTCTGAGCGCGGCTGCCCGTCTGCATAACGCAt                            >  1:1567550/1‑50 (MQ=255)                   gTTAGTCTTCGCCTCTATTCTGAGCGCGGCTGCCCGTCTGCATAACGCAt                            >  1:2053093/1‑50 (MQ=255)                    ttAGTCTTCGCCTCTATTCTGAGCGCGGCTGCCCGTCTGCATAACGCAtc                           >  1:1510682/1‑50 (MQ=255)                       gTCTTCGCCTCTATTCTGAGCGCGGCTGCCCGTCTGCATAACGCAtctc                         <  1:1027149/49‑1 (MQ=255)                       gTCTTCGCCTCTATTCTGAGCGCGGCTGCCCGTCTGCATAACGCAtctc                         <  1:1493101/49‑1 (MQ=255)                       gTCTTCGCCTCTATTCTGAGCGCGGCTGCCCGTCTGCATAACGCATCTCa                        <  1:723105/50‑1 (MQ=255)                       gTCTTCGCCTCTATTCTGAGCGCGGCTGCCCGTCTGCATAACGCATCTCa                        <  1:1381734/50‑1 (MQ=255)                        tCTTCGCCTCTATTCTGAGCGCGGCTGCCCGTCTGCATAACGCATCTCAg                       >  1:1820940/1‑50 (MQ=255)                         cTTCGCCTCTATTCTGAGCGCGGCTGCCCGTCTGCATAACGCATCTCAGt                      >  1:712957/1‑50 (MQ=255)                            cGCCTCTATTCTGAGCGCGGCTGCCCGTCTGCATAACGCATCTCAGt                      <  1:1978237/47‑1 (MQ=255)                            cGCCTCTATTCTGAGCGCGGCTGCCCGTCTGCATAACGCATCTCAGt                      <  1:2127786/47‑1 (MQ=255)                              ccTCTATTCTGAGCGCGGCTGCCCGTCTGCATAACGCATCTCAGTTAgg                  >  1:1632665/1‑49 (MQ=255)                              ccTCTATTCTGAGCGCGGCTGCCCGTCTGCATAACGCATCTCAGTTAgg                  >  1:2141543/1‑49 (MQ=255)                                 ctATTCTGAGCGCGGCTGCCCGTCTGCATAACGCATCTCAGTTAGGCaa               >  1:689992/1‑49 (MQ=255)                                  tATTCTGAGCGCGGCTGCCCGTCTGCATAACGCATCTCAGTTAGGCaaaa             >  1:289085/1‑50 (MQ=255)                                  tATTCTGAGCGCGGCTGCCCGTCTGCATAACGCATCTCAGTTAGGCaaaa             >  1:593396/1‑50 (MQ=255)                                    ttCTGAGCGCGGCTGCCCGTCTGCATAACGCATCTCAGTTAGGCaaaa             <  1:862417/48‑1 (MQ=255)                                    ttCTGAGCGCGGCTGCCCGTCTGCATAACGCATCTCAGTTAGGCAAAAt            >  1:186534/1‑49 (MQ=255)                                    ttCTGAGCGCGGCTGCCCGTCTGCATAACGCATCTCAGTTAGGCAAAATc           <  1:1768879/50‑1 (MQ=255)                                         aGCGCGGCTGCCCGTCTGCATAACGCATCTCAGTTAGGCAAAATCAGttt      >  1:1364376/1‑50 (MQ=255)                                         aGCGCGGCTGCCCGTCTGCATAACGCATCTCAGTTAGGCAAAATCAGttt      >  1:1432442/1‑50 (MQ=255)                                            gcgGCTGCCCGTCTGCATAACGCATCTCAGTTAGGCAAAATCAGTTTTCt   >  1:1173685/1‑50 (MQ=255)                                             cgGCTGCCCGTCTGCATAACGCATCTCAGTTAGGCAAAATCAGTTTTCTg  <  1:1167116/50‑1 (MQ=255)                                                |                                               GCAAATGATCGTTATGCCGTTAGTCTTCGCCTCTATTCTGAGCGCGGTTGCCCGTCTGCATAACGCATCTCAGTTAGGCAAAATCAGTTTTCTG  >  NC_000913/1811161‑1811254

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑