Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	1,898,152	G→A	A407A (GCG→GCA)	sdaA →	L‑serine deaminase I

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	1,898,152	0	G	A	100.0%	91.7 / NA	23	A407A (GCG→GCA)	sdaA	L‑serine deaminase I
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base A (13/10);  total (13/10)

TGCATTGAGCGTAATGCCATTGCCTCTGTGAAGGCGATTAACGCCGCGCGGATGGCTCTGCGCCGCACCAGTGCACCGCGCGTCTCGCTGGATA  >  NC_000913/1898105‑1898198                                                |                                               tGCATTGAGCGTAATGCCATTGCCTCTGTGAAGGCGATTAACGCcgcacg                                              <  1:604528/50‑1 (MQ=255)     ttGAGCGTAATGCCATTGCCTCTGTGAAGGCGATTAACGCCGCACGGATg                                          <  1:391108/50‑1 (MQ=255)     ttGAGCGTAATGCCATTGCCTCTGTGAAGGCGATTAACGCCGCACGGATg                                          >  1:939786/1‑50 (MQ=255)     ttGAGCGTAATGCCATTGCCTCTGTGAAGGCGATTAACGCCGCACGGATg                                          >  1:164221/1‑50 (MQ=255)       gAGCGTAATGCCATTGCCTCTGTGAAGGCGATTAACGCCGCACGGATGGc                                        >  1:1385872/1‑50 (MQ=255)            tAATGCCATTGCCTCTGTGAAGGCGATTAACGCCGCACGGATGGCTCTgc                                   <  1:1180239/50‑1 (MQ=255)            tAATGCCATTGCCTCTGTGAAGGCGATTAACGCCGCACGGATGGCTCTgc                                   <  1:1737836/50‑1 (MQ=255)             aaTGCCATTGCCTCTGTGAAGGCGATTAACGCCGCACGGATGGCTCTgcg                                  >  1:40342/1‑50 (MQ=255)             aaTGCCATTGCCTCTGTGAAGGCGATTAACGCCGCACGGATGGCTCTgcg                                  >  1:2075133/1‑50 (MQ=255)               tGCCATTGCCTCTGTGAAGGCGATTAACGCCGCACGGATGGCTCTGcgcc                                >  1:1116545/1‑50 (MQ=255)                gCCATTGACTCTGTGAAGGCGATTAACGCCGCACGGATGGCTCTGcgccg                               >  1:1618230/1‑50 (MQ=38)                   aTTGCCTCTGTGAAGGCGATTAACGCCGCACGGATGGCTCTGCGCCGCAc                            <  1:907455/50‑1 (MQ=255)                      gCCTCTGTGAAGGCGATTAACGCCGCACGGATGGCTCTGCGCCGCACCa                          <  1:947464/49‑1 (MQ=255)                        ctctGTGAAGGCGATTAACGCCGCACGGATGGCTCTGCGCCGCACCAGTg                       <  1:1522719/50‑1 (MQ=255)                         tctGTGAAGGCGATTAACGCCGCACGGATGGCTCTGCGCCGCACCAGTg                       >  1:156893/1‑49 (MQ=255)                         tctGTGAAGGCGATTAACGCCGCACGGATGGCTCTGCGCCGCACCAGTg                       >  1:696216/1‑49 (MQ=255)                               aaGGCGATTAACGCCGCACGGATGGCTCTGCGCCGCACCAGTGCACcgcg                >  1:1310205/1‑50 (MQ=255)                                 ggCGATTAACGCCGCACGGATGGCTCTGCGCCGCACCAGTGCACcgcgcg              >  1:284807/1‑50 (MQ=255)                                 ggCGATTAACGCCGCACGGATGGCTCTGCGCCGCACCAGTGCACcgcgcg              >  1:561758/1‑50 (MQ=255)                                  gCGATTAACGCCGCACGGATGGCTCTGCGCCGCACCAGTGCACCGCGCGt             <  1:1721003/50‑1 (MQ=255)                                     aTTAACGCCGCACGGATGGCTCTGCGCCGCACCAGTGCACCGCGCGtctc          <  1:2038840/50‑1 (MQ=255)                                        aaCGCCGCACGGATGGCTCTGCGCCGCACCAGTGCACCGCGCGTCTCGCt       >  1:1865628/1‑50 (MQ=255)                                            ccgcACGGATGGCTCTGCGCCGCACCAGTGCACCGCGCGTCTCGCTGGAt   <  1:340130/50‑1 (MQ=255)                                            ccgcACGGATGGCTCTGCGCCGCACCAGTGCACCGCGCGTCTCGCTGGAt   <  1:1612487/50‑1 (MQ=255)                                             cgcacgGATGGCTCTGCGCCGCACCAGTGCACCGCGCGTCTCGCTGGATa  <  1:1137352/50‑1 (MQ=255)                                                |                                               TGCATTGAGCGTAATGCCATTGCCTCTGTGAAGGCGATTAACGCCGCGCGGATGGCTCTGCGCCGCACCAGTGCACCGCGCGTCTCGCTGGATA  >  NC_000913/1898105‑1898198

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 18 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑