Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	1,941,784	A→G	V267A (GTC→GCC)	znuA ←	Zn(2(+)) ABC transporter periplasmic binding protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	1,941,784	0	A	G	100.0%	96.7 / NA	26	V267A (GTC→GCC)	znuA	Zn(2(+)) ABC transporter periplasmic binding protein
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base G (15/11);  total (15/11)

CCCCAGGGGATCCAACGTTCCCATACGAACGGATGTCCCTCGTGCGACGCTCTCAACGACCGCTGGCCTGAACTGTGGCTCAGCAAAAACG  >  NC_000913/1941738‑1941828                                               |                                             ccccAGGGGATCCAACGTTCCCATACGAACGGATGTCCCTCGTGCGgcgc                                           >  1:1181502/1‑50 (MQ=255)   ccAGGGGATCCAACGTTCCCATACGAACGGATGTCCCTCGTGCGGCGctc                                         >  1:948553/1‑50 (MQ=255)   ccAGGGGATCCAACGTTCCCATACGAACGGATGTCCCTCGTGCGGCGctc                                         <  1:1202060/50‑1 (MQ=255)      ggggATCCAACGTTCCCATACGAACGGATGTCCCTCGTGCGGCGCTCTCa                                      <  1:2238863/50‑1 (MQ=255)         gATCCAACGTTCCCATACGAACGGATGTCCCTCGTGCGGCGCTCTCAAc                                    >  1:2076217/1‑49 (MQ=255)         gATCCAACGTTCCCATACGAACGGATGTCCCTCGTGCGGCGCTCTCAAc                                    >  1:955434/1‑49 (MQ=255)         gATCCAACGTTCCCATACGAACGGATGTCCCTCGTGCGGCGCTCTCAACg                                   <  1:177130/50‑1 (MQ=255)           tCCAACGTTCCCATACGAACGGATGTCCCTCGTGCGGCGCTCTCAACGAc                                 >  1:1585163/1‑50 (MQ=255)               aCGTTCCCATACGAACGGATGTCCCTCGTGCGGCGCTCTCAACGACCGCt                             <  1:2196693/50‑1 (MQ=255)                   tCCCATACGAACGGATGTCCCTCGTGCGGCGCTCTCAACGACCGCTGGcc                         >  1:1955810/1‑50 (MQ=255)                       aTACGAACGGATGTCCCTCGTGCGGCGCTCTCAACGACCGCTGGCCTGaa                     >  1:1788431/1‑50 (MQ=255)                         aCGAACGGATGTCCCTCGTGCGGCGCTCTCAACGACCGCTGGCCTGAACt                   >  1:1991634/1‑50 (MQ=255)                         aCGAACGGATGTCCCTCGTGCGGCGCTCTCAACGACCGCTGGCCTGAACt                   >  1:358530/1‑50 (MQ=255)                          cGAACGGATGTCCCTCGTGCGGCGCTCTCAACGACCGCTGGCCTGAACt                   <  1:1201770/49‑1 (MQ=255)                           gAACGGATGTCCCTCGTGCGGCGCTCTCAACGACCGCTGGCCTGAACtgt                 <  1:1362327/50‑1 (MQ=255)                             aCGGATGTCCCTCGTGCGGCGCTCTCAACGACCGCTGGCCTGAACTGTgg               <  1:605647/50‑1 (MQ=255)                              cGGATGTCCCTCGTGCGGCGCTCTCAACGACCGCTGGCCTGAACTGTGGc              <  1:1379057/50‑1 (MQ=255)                               ggATGTCCCTCGTGCGGCGCTCTCAACGACCGCTGGCCTGAACTGTGGCt             >  1:239615/1‑50 (MQ=255)                                gATGTCCCTCGTGCGGCGCTCTCAACGACCGCTGGCCTGAACTGTGGCt             >  1:1622589/1‑49 (MQ=255)                                gATGTCCCTCGTGCGGCGCTCTCAACGACCGCTGGCCTGAACTGTGGCTc            <  1:1211242/50‑1 (MQ=255)                                 aTGTCCCTCGTGCGGCGCTCTCAACGACCGCTGGCCTGAACTGTGGCt             >  1:1790851/1‑48 (MQ=255)                                 aTGTCCCTCGTGCGGCGCTCTCAACGACCGCTGGCCTGAACTGTGGCt             >  1:2023577/1‑48 (MQ=255)                                  tGTCCCTCGTGCGGCGCTCTCAACGACCGCTGGCCTGAACTGTGGCTCAg          >  1:80875/1‑50 (MQ=255)                                  tGTCCCTCGTGCGGCGCTCTCAACGACCGCTGGCCTGAACTGTGGCTCAg          <  1:960194/50‑1 (MQ=255)                                    tCCCTCGTGCGGCGCTCTCAACGACCGCTGGCCTGAACTGTGGCTCAGCa        <  1:1224266/50‑1 (MQ=255)                                       cTCGTGCGGCGCTCTCAACGACCGCTGGCCTGAACTGTGGCTCAGCaaa      >  1:1395575/1‑49 (MQ=255)                                           tGCGGCGCTCTCAACGACCGCTGGCCTGAACTGTGGCTCAGCAAAAACg  >  1:1133103/1‑49 (MQ=255)                                               |                                             CCCCAGGGGATCCAACGTTCCCATACGAACGGATGTCCCTCGTGCGACGCTCTCAACGACCGCTGGCCTGAACTGTGGCTCAGCAAAAACG  >  NC_000913/1941738‑1941828

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑