Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	2,040,801	T→C	L107L (TTA→CTA)	msrQ →	periplasmic protein‑L‑methionine sulfoxide reductase heme binding subunit

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	2,040,801	0	T	C	100.0%	58.4 / NA	16	L107L (TTA→CTA)	msrQ	periplasmic protein‑L‑methionine sulfoxide reductase heme binding subunit
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base C (11/5);  total (11/5)

ATTTAACAAGTTACGCATTGCTGGAGCTGGGCGTGAATAATCTGGCGTTATTAGGTAAGGAGTTAATTACCCGGCCTTATTTAACGTTAGG  >  NC_000913/2040754‑2040844                                                |                                            aTTTAACAAGTTACGCATTGCTGGAGCTGGGCGTGAATAATCTGGCGcta                                           >  1:36744/1‑50 (MQ=255) aTTTAACAAGTTACGCATTGCTGGAGCTGGGCGTGAATAATCTGGCGcta                                           >  1:1758915/1‑50 (MQ=255)  tttAACAAGTTACGCATTGCTGGAGCTGGGCGTGAATAATCTGGCGctat                                          >  1:147934/1‑50 (MQ=255)     aaCAAGTTACGCATTGCTGGAGCTGGGCGTGAATAATCTGGCGctatta                                        <  1:109925/49‑1 (MQ=255)       cAAGTTACGCATTGCTGGAGCTGGGCGTGAATAATCTGGCGCTATTAgg                                      >  1:1763340/1‑49 (MQ=255)        aaGTTACGCATTGCTGGAGCTGGGCGTGAATAATCTGGCGCTATTAGGTa                                    <  1:1504156/50‑1 (MQ=255)          gTTACGCATTGCTGGAGCTGGGCGTGAATAATCTGGCGCTATTAGGTAAg                                  <  1:1310122/50‑1 (MQ=255)                cATTGCTGGAGCTGGGCGTGAATAATCTGGCGCTATTAGGTAAGGAGTTa                            <  1:347768/50‑1 (MQ=255)                   tGCTGGAGCTGGGCGTGAATAATCTGGCGCTATTAGGTAAGGAGTTAAt                          >  1:1278367/1‑49 (MQ=255)                      tGGAGCTGGGCGTGAATAATCTGGCGCTATTAGGTAAGGAGTTAATTAcc                      >  1:999494/1‑50 (MQ=255)                         aGCTGGGCGTGAATAATCTGGCGCTATTAGGTAAGGAGTTAATTACCCgg                   >  1:1879662/1‑50 (MQ=255)                           cTGGGCGTGAATAATCTGGCGCTATTAGGTAAGGAGTTAATTACCCGGcc                 >  1:158234/1‑50 (MQ=255)                           cTGGGCGTGAATAATCTGGCGCTATTAGGTAAGGAGTTAATTACCCGGcc                 >  1:310829/1‑50 (MQ=255)                            tGGGCGTGAATAATCTGGCGCTATTAGGTAAGGAGTTAATTACCCGGCCt                >  1:768739/1‑50 (MQ=255)                              ggCGTGAATAATCTGGCGCTATTAGGTAAGGAGTTAATTACCCGGCCtt               <  1:256866/49‑1 (MQ=255)                               gCGTGAATAATCTGGCGCTATTAGGTAAGGAGTTAATTACCCGGCCTTAt             >  1:1478129/1‑50 (MQ=255)                                  tGAATAATCTGGCGCTATTAGGTAAGGAGTTAATTACCCGGCCTTAttt           >  1:2120463/1‑49 (MQ=255)                                       aatCTGGCGCTATTAGGTAAGGAGTTAATTACCCGGCCTTATTTAACGt      >  1:1411484/1‑49 (MQ=255)                                        atCTGGCGCTATTAGGTAAGGAGTTAATTACCCGGCCTTATTTAACGtt     <  1:1526420/49‑1 (MQ=255)                                           tGGCGCTATTAGGTAAGGAGTTAATTACCCGGCCTTATTTAACGTTAgg  >  1:154309/1‑49 (MQ=255)                                             gcgcTATTAGGTAAGGAGTTAATTACCCGGCCTTATTTAACGTTAgg  <  1:91399/47‑1 (MQ=255)                                                |                                            ATTTAACAAGTTACGCATTGCTGGAGCTGGGCGTGAATAATCTGGCGTTATTAGGTAAGGAGTTAATTACCCGGCCTTATTTAACGTTAGG  >  NC_000913/2040754‑2040844

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑