Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	214,885	C→T	T18I (ACC→ATC)	arfB →	peptidyl‑tRNA hydrolase, ribosome rescue factor

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	214,885	0	C	T	100.0%	118.5 / NA	31	T18I (ACC→ATC)	arfB	peptidyl‑tRNA hydrolase, ribosome rescue factor
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base T (15/16);  total (15/16)

TGTGATTTCCCGACATGTTGCTATTCCCGATGGTGAGCTTGAGATCACCGCCATTCGTGCGCAGGGCGCGGGCGGGCAGCATGTTAATAAGACC  >  NC_000913/214838‑214931                                                |                                               tgtgATTTCCCGACATGTTGCTATTCCCGATGGTGAGCTTGAGATCATCg                                              >  1:2119258/1‑50 (MQ=255)  gtgATTTCCCGACATGTTGCTATTCCCGATGGTGAGCTTGAGATCATCGc                                             >  1:1299681/1‑50 (MQ=255)   tgATTTCCCGACATGTTGCTATTCCCGATGGTGAGCTTGAGATCATCGcc                                            <  1:1549777/50‑1 (MQ=255)   tgATTTCCCGACATGTTGCTATTCCCGATGGTGAGCTTGAGATCATCGc                                             <  1:442107/49‑1 (MQ=255)        tCCCGACATGTTGCTATTCCCGATGGTGAGCTTGAGATCATCGCCATTc                                        <  1:676322/49‑1 (MQ=255)          ccGACATGTTGCTATTCCCGATGGTGAGCTTGAGATCATCGCCATTCGt                                      <  1:1253802/49‑1 (MQ=255)          ccGACATGTTGCTATTCCCGATGGTGAGCTTGAGATCATCGCCATTCGTg                                     <  1:376097/50‑1 (MQ=255)          ccGACATGTTGCTATTCCCGATGGTGAGCTTGAGATCATCGCCATTCGTg                                     <  1:1411849/50‑1 (MQ=255)            gACATGTTGCTATTCCCGATGGTGAGCTTGAGATCATCGCCATTCGTgcg                                   >  1:1988074/1‑50 (MQ=255)             aCATGTTGCTATTCCCGATGGTGAGCTTGAGATCATCGCCATTCGTgcg                                   >  1:454359/1‑49 (MQ=255)             aCATGTTGCTATTCCCGATGGTGAGCTTGAGATCATCGCCATTCGTgcg                                   >  1:392522/1‑49 (MQ=255)               aTGTTGCTATTCCCGATGGTGAGCTTGAGATCATCGCCATTCGTGCGCAg                                >  1:503018/1‑50 (MQ=255)                tGTTGCTATTCCCGATGGTGAGCTTGAGATCATCGCCATTCGTGCGCAgg                               >  1:1900491/1‑50 (MQ=255)                tGTTGCTATTCCCGATGGTGAGCTTGAGATCATCGCCATTCGTGCGCAgg                               >  1:1910961/1‑50 (MQ=255)                tGTTGCTATTCCCGATGGTGAGCTTGAGATCATCGCCATTCGTGCGCAgg                               <  1:757878/50‑1 (MQ=255)                tGTTGCTATTCCCGATGGTGAGCTTGAGATCATCGCCATTCGTGCGCAgg                               <  1:2036627/50‑1 (MQ=255)                  ttGCTATTCCCGATGGTGAGCTTGAGATCATCGCCATTCGTGCGCAggg                              <  1:65401/49‑1 (MQ=255)                     cTATTCCCGATGGTGAGCTTGAGATCATCGCCATTCGTGCGCAGGGcgcg                          >  1:800612/1‑50 (MQ=255)                       aTTCCCGATGGTGAGCTTGAGATCATCGCCATTCGTGCGCAGGGCgcggg                        >  1:2122913/1‑50 (MQ=255)                       aTTCCCGATGGTGAGCTTGAGATCATCGCCATTCGTGCGCAGGGCgcggg                        >  1:1929301/1‑50 (MQ=255)                       aTTCCCGATGGTGAGCTTGAGATCATCGCCATTCGTGCGCAGGGCgcggg                        >  1:1581616/1‑50 (MQ=255)                          cccGATGGTGAGCTTGAGATCATCGCCATTCGTGCGCAGGGCgcgggcgg                     >  1:918449/1‑50 (MQ=255)                            cGATGGTGAGCTTGAGATCATCGCCATTCGTGCGCAGGGCGCgggcgggc                   <  1:230222/50‑1 (MQ=255)                             gATGGTGAGCTTGAGATCATCGCCATTCGTGCGCAGGGCGcgggcggg                    >  1:1983754/1‑48 (MQ=255)                              aTGGTGAGCTTGAGATCATCGCCATTCGTGCGCAGGGCGCGGGCGGgcag                 >  1:1380923/1‑50 (MQ=255)                                  tGAGCTTGAGATCATCGCCATTCGTGCGCAGGGCGCGGGCGGGCAGCATg             <  1:782904/50‑1 (MQ=255)                                    aGCTTGAGATCATCGCCATTCGTGCGCAGGGCGCGGGCGGGCAGCATGtt           <  1:261198/50‑1 (MQ=255)                                     gCTTGAGATCATCGCCATTCGTGCGCAGGGCGCGGGCGGGCAGCATGTta          <  1:1618973/50‑1 (MQ=255)                                         gagaTCATCGCCATTCGTGCGCAGGGCGCGGGCGGGCAGCATGTtaata       <  1:1935263/49‑1 (MQ=255)                                          agaTCATCGCCATTCGTGCGCAGGGCGCGGGCGGGCAGCATGTTAATAAg     <  1:41416/50‑1 (MQ=255)                                            atcatcGCCATTCGTGCGCAGGGCGCGGGCGGGCAGCATGTTAATAAGAc   <  1:273765/50‑1 (MQ=255)                                             tCATCGCCATTCGTGCGCAGGGCGCGGGCGGGCAGCATGTTAATAAGAcc  <  1:1473831/50‑1 (MQ=255)                                                |                                               TGTGATTTCCCGACATGTTGCTATTCCCGATGGTGAGCTTGAGATCACCGCCATTCGTGCGCAGGGCGCGGGCGGGCAGCATGTTAATAAGACC  >  NC_000913/214838‑214931

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑