Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	2,662,004	T→C	E43G (GAA→GGA)	iscR ←	DNA‑binding transcriptional dual regulator IscR

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	2,662,004	0	T	C	100.0%	139.2 / NA	36	E43G (GAA→GGA)	iscR	DNA‑binding transcriptional dual regulator IscR
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base C (20/16);  total (20/16)

GTACGCTGGAAACCAGACCATTTTTACGCAGACGGGAAAACAGTTGTTCCAGATAAGAAAGGGAAATTCCCTGACGTTCGGAAATATCAGCC  >  NC_000913/2661957‑2662048                                                |                                             gTACGCTGGAAACCAGACCATTTTTACGCAGACGGGAAAACAGTTGTccc                                            <  1:2112186/50‑1 (MQ=255)  tACGCTGGAAACCAGACCATTTTTACGCAGACGGGAAAACAGTTGTCCCa                                           <  1:815870/50‑1 (MQ=255)      cTGGAAACCAGACCATTTTTACGCAGACGGGAAAACAGTTGTCCCAGATa                                       <  1:862282/50‑1 (MQ=255)      cTGGAAACCAGACCATTTTTACGCAGACGGGAAAACAGTTGTCCCAGATa                                       <  1:59907/50‑1 (MQ=255)         gAAACCAGACCATTTTTACGCAGACGGGAAAACAGTTGTCCCAGATAAg                                     >  1:803822/1‑49 (MQ=255)         gAAACCAGACCATTTTTACGCAGACGGGAAAACAGTTGTCCCAGATAAGa                                    <  1:2029952/50‑1 (MQ=255)           aaCCAGACCATTTTTACGCAGACGGGAAAACAGTTGTCCCAGATAAGaaa                                  <  1:687853/50‑1 (MQ=255)              cAGACCATTTTTACGCAGACGGGAAAACAGTTGTCCCAGATAAGAAAgg                                >  1:1042733/1‑49 (MQ=255)                   cATTTTTACGCAGACGGGAAAACAGTTGTCCCAGATAAGAAAGGGAAAtt                          <  1:1377704/50‑1 (MQ=255)                   cATTTTTACGCAGACGGGAAAACAGTTGTCCCAGATAAGAAAGGGAAAtt                          <  1:544751/50‑1 (MQ=255)                   cATTTTTACGCAGACGGGAAAACAGTTGTCCCAGATAAGAAAGGGAAAtt                          <  1:487454/50‑1 (MQ=255)                    aTTTTTACGCAGACGGGAAAACAGTTGTCCCAGATAAGAAAGGGAAATTc                         >  1:1460612/1‑50 (MQ=255)                     tttttACGCAGACGGGAAAACAGTTGTCCCAGATAAGAAAGGGAAATTcc                        <  1:401857/50‑1 (MQ=255)                       tttACGCAGACGGGAAAACAGTTGTCCCAGATAAGAAAGGGAAATTccc                       <  1:601188/49‑1 (MQ=255)                       tttACGCAGACGGGAAAACAGTTGTCCCAGATAAGAAAGGGAAATTCCCt                      >  1:940544/1‑50 (MQ=255)                       tttACGCAGACGGGAAAACAGTTGTCCCAGATAAGAAAGGGAAATTCCCt                      >  1:508725/1‑50 (MQ=255)                       tttACGCAGACGGGAAAACAGTTGTCCCAGATAAGAAAGGGAAATTCCCt                      >  1:1988722/1‑50 (MQ=255)                          aCGCAGACGGGAAAACAGTTGTCCCAGATAAGAAAGGGAAATTCCCTGa                    <  1:1556053/49‑1 (MQ=255)                           cGCAGACGGGAAAACAGTTGTCCCAGATAAGAAAGGGAAATTCCCTGAc                   <  1:1353569/49‑1 (MQ=255)                           cGCAGACGGGAAAACAGTTGTCCCAGATAAGAAAGGGAAATTCCCTGACg                  >  1:1489854/1‑50 (MQ=255)                            gCAGACGGGAAAACAGTTGTCCCAGATAAGAAAGGGAAATTCCCTGACGt                 >  1:1787081/1‑50 (MQ=255)                             cAGACGGGAAAACAGTTGTCCCAGATAAGAAAGGGAAATTCCCTGACGtt                >  1:1013950/1‑50 (MQ=255)                             cAGACGGGAAAACAGTTGTCCCAGATAAGAAAGGGAAATTCCCTGACGtt                >  1:702471/1‑50 (MQ=255)                              aGACGGGAAAACAGTTGTCCCAGATAAGAAAGGGAAATTCCCTGACGTTc               >  1:775514/1‑50 (MQ=255)                               gACGGGAAAACAGTTGTCCCAGATAAGAAAGGGAAATTCCCTGACGTTCg              >  1:619624/1‑50 (MQ=255)                                 cGGGAAAACAGTTGTCCCAGATAAGAAAGGGAAAGTCCCTGACGTTCGGa            <  1:1661132/50‑1 (MQ=255)                                  gggAAAACAGTTGTCCCAGATAAGAAAGGGAAATTCCCTGACGTTCGGa            >  1:866408/1‑49 (MQ=255)                                  gggAAAACAGTTGTCCCAGATAAGAAAGGGAAATTCCCTGACGTTCGGa            >  1:230730/1‑49 (MQ=255)                                  gggAAAACAGTTGTCCCAGATAAGAAAGGGAAATTCCCTGACGTTCGGa            >  1:1467031/1‑49 (MQ=255)                                   ggAAAACAGTTGTCCCAGATAAGAAAGGGAAATTCCCTGACGTTCGGaaa          >  1:1630741/1‑50 (MQ=255)                                    gAAAACAGTTGTCCCAGATAAGAAAGGGAAATTCCCTGACGTTCGGaaa          >  1:1645705/1‑49 (MQ=255)                                      aaaCAGTTGTCCCAGATAAGAAAGGGAAATTCCCTGACGTTCGGAAatat       <  1:597931/50‑1 (MQ=255)                                      aaaCAGTTGTCCCAGATAAGAAAGGGAAATTCCCTGACGTTCGGAAatat       >  1:2216373/1‑50 (MQ=255)                                      aaaCAGTTGTCCCAGATAAGAAAGGGAAATTCCCTGACGTTCGGAAatat       >  1:191072/1‑50 (MQ=255)                                       aaCAGTTGTCCCAGATAAGAAAGGGAAATTCCCTGACGTTCGGAAATATc      <  1:2067250/50‑1 (MQ=255)                                         cAGTTGTCCCAGATAAGAAAGGGAAATTCCCTGACGTTCGGAAATATCAg    <  1:1826351/50‑1 (MQ=255)                                          aGTTGTCCCAGATAAGAAAGGGAAATTCCCTGACGTTCGGAAATATCAGc   >  1:1516998/1‑50 (MQ=255)                                           gttgtcCCAGATAAGAAAGGGAAATTCCCTGACGTTCGGAAATATCAGcc  <  1:377175/50‑1 (MQ=255)                                                |                                             GTACGCTGGAAACCAGACCATTTTTACGCAGACGGGAAAACAGTTGTTCCAGATAAGAAAGGGAAATTCCCTGACGTTCGGAAATATCAGCC  >  NC_000913/2661957‑2662048

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 31 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑