Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	2,813,364	T→C	V250A (GTC→GCC)	emrB →	multidrug efflux pump membrane subunit EmrB

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	2,813,364	0	T	C	100.0%	107.6 / NA	29	V250A (GTC→GCC)	emrB	multidrug efflux pump membrane subunit EmrB
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base C (12/17);  total (12/17)

ATCATCCTTACCGTGGTGGCGGTGGTGGCTATCTGCTTCCTGATTGTCTGGGAGCTGACCGACGATAACCCGATAGTCGATCTGTCGTT  >  NC_000913/2813318‑2813406                                               |                                           atcatcCTTACCGTGGTGGCGGTGGTGGCTATCTGCTTCCTGATTGCCTg                                         >  1:2098823/1‑50 (MQ=255)  tcatcCTTACCGTGGTGGCGGTGGTGGCTATCTGCTTCCTGATTGCCTgg                                        >  1:1134427/1‑50 (MQ=255)  tcatcCTTACCGTGGTGGCGGTGGTGGCTATCTGCTTCCTGATTGCCTgg                                        >  1:577277/1‑50 (MQ=255)  tcatcCTTACCGTGGTGGCGGTGGTGGCTATCTGCTTCCTGATTGCCTgg                                        <  1:2076115/50‑1 (MQ=255)    atcCTTACCGTGGTGGCGGTGGTGGCTATCTGCTTCCTGATTGCCTgg                                        >  1:841012/1‑48 (MQ=255)          aCCGTGGTGGCGGTGGTGGCTATCTGCTTCCTGATTGCCTGGGAGCTGAc                                >  1:1509129/1‑50 (MQ=255)             gtggtgGCGGTGGTGGCTATCTGCTTCCTGATTGCCTGGGAGCTGACcga                             >  1:1372142/1‑50 (MQ=255)                gtggCGGTGGTGGCTATCTGCTTCCTGATTGCCTGGGAGCTGACcgacga                          <  1:1714017/50‑1 (MQ=255)                gtggCGGTGGTGGCTATCTGCTTCCTGATTGCCTGGGAGCTGACcgacg                           <  1:1520735/49‑1 (MQ=255)                   gCGGTGGTGGCTATCTGCTTCCTGATTGCCTGGGAGCTGACCGACGATaa                       <  1:2166873/50‑1 (MQ=255)                    cGGTGGTGGCTATCTGCTTCTTGATTGCCTGGGAGCTGACCGACGATAAc                      >  1:17301/1‑50 (MQ=255)                    cGGTGGTGGCTATCTGCTTCCTGATTGCCTGGGAGCTGACCGACGATAAc                      >  1:1582385/1‑50 (MQ=255)                      gtggtgGCTATCTGCTTCCTGATTGCCTGGGAGCTGACCGACGATAAccc                    <  1:1617304/50‑1 (MQ=255)                      gtggtgGCTATCTGCTTCCTGATTGCCTGGGAGCTGACCGACGATAAccc                    <  1:1801596/50‑1 (MQ=255)                      gtggtgGCTATCTGCTTCCTGATTGCCTGGGAGCTGACCGACGATAAccc                    <  1:1487202/50‑1 (MQ=255)                          tggCTATCTGCTTCCTGATTGCCTGGGAGCTGACCGACGATAACCCGATa                <  1:829891/50‑1 (MQ=255)                          tggCTATCTGCTTCCTGATTGCCTGGGAGCTGACCGACGATAACCCGATa                <  1:714506/50‑1 (MQ=255)                            gCTATCTGCTTCCTGATTGCCTGGGAGCTGACCGACGATAACCCGATAGt              <  1:521622/50‑1 (MQ=255)                            gCTATCTGCTTCCTGATTGCCTGGGAGCTGACCGACGATAACCCGATAGt              <  1:690369/50‑1 (MQ=255)                             cTATCTGCTTCCTGATTGCCTGGGAGCTGACCGACGATAACCCGATAGTc             <  1:101349/50‑1 (MQ=255)                             cTATCTGCTTCCTGATTGCCTGGGAGCTGACCGACGATAACCCGATAGTc             <  1:1389274/50‑1 (MQ=255)                             cTATCTGCTTCCTGATTGCCTGGGAGCTGACCGACGATAACACGATAGTc             >  1:1431587/1‑50 (MQ=255)                               aTCTGCTTCCTGATTGCCTGGGAGCTGACCGACGATAACCCGATAGTCGa           >  1:2188105/1‑50 (MQ=255)                               aTCTGCTTCCTGATTGCCTGGGAGCTGACCGACGATAACCCGATAGTCGa           >  1:261575/1‑50 (MQ=255)                                    cTTCCTGATTGCCTGGGAGCTGACCGACGATAACCCGATAGTCGATc         <  1:1888187/47‑1 (MQ=255)                                    cTTCCTGATTGCCTGGGAGCTGACCGACGATAACCCGATAGTCGATCTGt      <  1:8324/50‑1 (MQ=255)                                      tCCTGATTGCCTGGGAGCTGACCGACGATAACCCGATAGTCGATCTGTCg    >  1:828051/1‑50 (MQ=255)                                       ccTGATTGCCTGGGAGCTGACCGACGATAACCCGATAGTCGATCTGTCg    <  1:1085155/49‑1 (MQ=255)                                        cTGATTGCCTGGGAGCTGACCGACGATAACCCGATAGTCGATCTGTCgtt  <  1:2030877/50‑1 (MQ=255)                                               |                                           ATCATCCTTACCGTGGTGGCGGTGGTGGCTATCTGCTTCCTGATTGTCTGGGAGCTGACCGACGATAACCCGATAGTCGATCTGTCGTT  >  NC_000913/2813318‑2813406

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 20 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑