Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	3,090,282	C→T	intergenic (+30/‑65)	yggI → / → endA	conserved protein YggI/DNA‑specific endonuclease I

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	3,090,282	0	C	T	100.0%	139.6 / NA	35	intergenic (+30/‑65)	yggI/endA	conserved protein YggI/DNA‑specific endonuclease I
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base T (14/21);  total (14/21)

AGCTGGTTGCGAAATAACCATCTGAACTATCAGGAACTTTCCTGATCTGGCTGATTGCATACCAAAACAGCTTTCGCTACGTTGCTGGCT  >  NC_000913/3090236‑3090325                                               |                                            aGCTGGTTGCGAAATAACCATCTGAACTATCAGGAACTTTCCTGATTTgg                                          <  1:1592206/50‑1 (MQ=255)    tGGTTGCGAAATAACCATCTGAACTATCAGGAACTTTCCTGATTTGGCt                                        >  1:637315/1‑49 (MQ=255)      gTTGCGAAATAACCATCTGAACTATCAGGAACTTTCCTGATTTGGCTg                                       <  1:906962/48‑1 (MQ=255)      gTTGCGAAATAACCATCTGAACTATCAGGAACTTTCCTGATTTGGCTg                                       <  1:2008520/48‑1 (MQ=255)      gTTGCGAAATAACCATCTGAACTATCAGGAACTTTCCTGATTTGGCTGAt                                     >  1:431171/1‑50 (MQ=255)       ttGCGAAATAACCATCTGAACTATCAGGAACTTTCCTGATTTGGCTGAtt                                    >  1:749515/1‑50 (MQ=255)         gCGAAATAACCATCTGAACTATCAGGAACTTTCCTGATTTGGCTGATTg                                   >  1:1550469/1‑49 (MQ=255)          cGAAATAACCATCTGAACTATCAGGAACTTTCCTGATTTGGCTGATTGCa                                 <  1:1386291/50‑1 (MQ=255)            aaaTAACCATCTGAACTATCAGGAACTTTCCTGATTTGGCTGATTGCATa                               <  1:100173/50‑1 (MQ=255)            aaaTAACCATCTGAACTATCAGGAACTTTCCTGATTTGGCTGATTGCATa                               <  1:1998582/50‑1 (MQ=255)             aaTAACCATCTGAACTATCAGGAACTTTCCTGATTTGGCTGATTGCATAc                              <  1:783848/50‑1 (MQ=255)             aaTAACCATCTGAACTATCAGGAACTTTCCTGATTTGGCTGATTGCATAc                              >  1:1833885/1‑50 (MQ=255)               tAACCATCTGAACTATCAGGAACTTTCCTGATTTGGCTGATTGCATACCa                            <  1:445731/50‑1 (MQ=255)                  ccATCTGAACTATCAGGAACTTTCCTGATTTGGCTGATTGCATACCaaaa                         >  1:1033145/1‑50 (MQ=255)                  ccATCTGAACTATCAGGAACTTTCCTGATTTGGCTGATTGCATACCaaaa                         <  1:808038/50‑1 (MQ=255)                  ccATCTGAACTATCAGGAACTTTCCTGATTTGGCTGATTGCATACCaaaa                         <  1:1186284/50‑1 (MQ=255)                    aTCTGAACTATCAGGAACTTTCCTGATTTGGCTGATTGCATACCAAAACa                       <  1:803046/50‑1 (MQ=255)                      cTGAACTATCAGGAACTTTCCTGATTTGGCTGATTGCATACCAAAACAGc                     >  1:199148/1‑50 (MQ=255)                        gAACTATCAGGAACTTTCCTGATTTGGCTGATTGCATACCAAAACAGCtt                   >  1:677856/1‑50 (MQ=255)                         aaCTATCAGGAACTTTCCTGATTTGGCTGATTGCATACCAAAACAGCttt                  <  1:54242/50‑1 (MQ=255)                         aaCTATCAGGAACTTTCCTGATTTGGCTGATTGCATACCAAAACAGCtt                   <  1:588531/49‑1 (MQ=255)                          aCTATCAGGAACTTTCCTGATTTGGCTGATTGCATACCAAAACAGCTTTc                 <  1:1958960/50‑1 (MQ=255)                          aCTATCAGGAACTTTCCTGATTTGGCTGATTGCATACCAAAACAGCTTTc                 >  1:167652/1‑50 (MQ=255)                              tCAGGAACTTTCCTGATTTGGCTGATTGCATACCAAAACAGCTTTCGc               <  1:276182/48‑1 (MQ=255)                              tCAGGAACTTTCCTGATTTGGCTGATTGCATACCAAAACAGCTTTCGCt              <  1:1986211/49‑1 (MQ=255)                              tCAGGAACTTTCCTGATTTGGCTGATTGCATACCAAAACAGCTTTCGCt              <  1:1870931/49‑1 (MQ=255)                              tCAGGAACTTTCCTGATTTGGCTGATTGCATACCAAAACAGCTTTCGCTa             <  1:2235943/50‑1 (MQ=255)                              tCAGGAACTTTCCTGATTTGGCTGATTGCATACCAAAACAGCTTTCGCTa             >  1:860836/1‑50 (MQ=255)                                aGGAACTTTCCTGATTTGGCTGATTGCATACCAAAACAGCTTTCGCTAc            <  1:2046312/49‑1 (MQ=255)                                aGGAACTTTCCTGATTTGGCTGATTGCATACCAAAACAGCTTTCGCTACg           >  1:846332/1‑50 (MQ=255)                                aGGAACTTTCCTGATTTGGCTGATTGCATACCAAAACAGCTTTCGCTACg           <  1:12508/50‑1 (MQ=255)                                  gAACTTTCCTGATTTGGCTGATTGCATACCAAAACAGCTTTCGCTACGtt         >  1:1115394/1‑50 (MQ=255)                                   aaCTTTCCTGATTTGGCTGATTGCATACCAAAACAGCTTTCGCTACGTTg        >  1:928341/1‑50 (MQ=255)                                       ttCCTGATTTGGCTGATTGCATACCAAAACAGCTTTCGCTACGTTGCTgg    >  1:1506575/1‑50 (MQ=255)                                          cTGATTTGGCTGATTGCATACCAAAACAGCTTTCGCTACGTTGCTGGCt  <  1:243764/49‑1 (MQ=255)                                               |                                            AGCTGGTTGCGAAATAACCATCTGAACTATCAGGAACTTTCCTGATCTGGCTGATTGCATACCAAAACAGCTTTCGCTACGTTGCTGGCT  >  NC_000913/3090236‑3090325

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑