Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	3,477,150	C→T	K90K (AAG→AAA)	fkpA ←	peptidyl‑prolyl cis‑trans isomerase FkpA

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	3,477,150	0	C	T	100.0%	132.5 / NA	34	K90K (AAG→AAA)	fkpA	peptidyl‑prolyl cis‑trans isomerase FkpA
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base T (13/21);  total (13/21)

CGAATGCTTGTAGAGTCTGTTCGATCTCTTGGTCGGAGAGTTTGCTCTTATCAGCAAATGCATCCTGAACACCAGCGATCAGCTGATCTTTA  >  NC_000913/3477104‑3477195                                               |                                              cGAATGCTTGTAGAGTCTGTTCGATCTCTTGGTCGGAGAGTTTGCtttt                                             <  1:1311373/49‑1 (MQ=255) cGAATGCTTGTAGAGTCTGTTCGATCTCTTGGTCGGAGAGTTTGCTTTTa                                            >  1:1042793/1‑50 (MQ=255)    aTGCTTGTAGAGTCTGTTCGATCTCTTGGTCGGAGAGTTTGCTTTTATCa                                         <  1:1916221/50‑1 (MQ=255)     tGCTTGTAGAGTCTGTTCGATCTCTTGGTCGGAGAGTTTGCTTTTATCAg                                        <  1:553507/50‑1 (MQ=255)     tGCTTGTAGAGTCTGTTCGATCTCTTGGTCGGAGAGTTTGCTTTTATCAg                                        <  1:650975/50‑1 (MQ=255)       cTTGTAGAGTCTGTTCGATCTCTTGGTCGGAGAGTTTGCTTTTATCAGc                                       <  1:1791330/49‑1 (MQ=255)       cTTGTAGAGTCTGTTCGATCTCTTGGTCGGAGAGTTTGCTTTTATCAGCa                                      <  1:40462/50‑1 (MQ=255)          gTAGAGTCTGTTCGATCTCTTGGTCGGAGAGTTTGCTTTTATCAGCaa                                     <  1:2192185/48‑1 (MQ=255)          gTAGAGTCTGTTCGATCTCTTGGTCGGAGAGTTTGCTTTTATCAGCAAAt                                   >  1:1812965/1‑50 (MQ=255)            agagTCTGTTCGATCTCTTGGTCGGAGAGTTTGCTTTTATCAGCAAATg                                  >  1:543239/1‑49 (MQ=255)                tCTGTTCGATCTCTTGGTCGGAGAGTTTGCTTTTATCAGCAAATGCATcc                             >  1:1401028/1‑50 (MQ=255)                tCTGTTCGATCTCTTGGTCGGAGAGTTTGCTTTTATCAGCAAATGCATcc                             >  1:1898709/1‑50 (MQ=255)                tCTGCTCGATCTCTTGGTCGGAGAGTTTGCTTTTATCAGCAAATGCATcc                             <  1:1361467/50‑1 (MQ=255)                    ttCGATCTCTTGGTCGGAGAGTTTGCTTTTATCAGCAAATGCATCCt                            <  1:285607/47‑1 (MQ=255)                    ttCGATCTCTTGGTCGGAGAGTTTGCTTTTATCAGCAAATGCATCCTGaa                         <  1:1443955/50‑1 (MQ=255)                    ttCGATCTCTTGGTCGGAGAGTTTGCTTTTATCAGCAAATGCATCCTGaa                         <  1:1523998/50‑1 (MQ=255)                      cGATCTCTTGGTCGGAGAGTTTGCTTTTATCAGCAAATGCATCCTGAac                        <  1:631835/49‑1 (MQ=255)                        aTCTCTTGGTCGGAGAGTTTGCTTTTATCAGCAAATGCATCCTGAacac                      >  1:1207869/1‑49 (MQ=255)                             ttGGTCGGAGAGTTTGCTTTTATCAGCAAATGCATCCTGAACACCAg                   <  1:1343005/47‑1 (MQ=255)                               ggTCGGAGAGTTTGCTTTTATCAGCAAATGCATCCTGAACACCAGCg                 <  1:1134221/47‑1 (MQ=255)                               ggTCGGAGAGTTTGCTTTTATCAGCAAATGCATCCTGAACACCAGCGAt               >  1:1352192/1‑49 (MQ=255)                                gTCGGAGAGTTTGCTTTTATCAGCAAATGCGTCCTGAACACCAGCGAt               >  1:917150/1‑48 (MQ=255)                                  cGGAGAGTTTGCTTTTATCAGCAAATGCATCCTGAACACCAGCGATCAGc           >  1:1859668/1‑50 (MQ=255)                                   ggAGAGTTTGCTTTTATCAGCAAATGCATCCTGAACACCAGCGATCAGc           <  1:451560/49‑1 (MQ=255)                                   ggAGAGTTTGCTTTTATCAGCAAATGCATCCTGAACACCAGCGATCAGCt          <  1:317465/50‑1 (MQ=255)                                    gagaGTTTGCTTTTATCAGCAAATGCATCCTGAACACCAGCGATCAGCTg         <  1:104179/50‑1 (MQ=255)                                     agagTTTGCTTTTATCAGCAAATGCATCCTGAACACCAGCGATCAGCTGa        >  1:2064867/1‑50 (MQ=255)                                     agagTTTGCTTTTATCAGCAAATGCATCCTGAACACCAGCGATCAGCTGa        >  1:267775/1‑50 (MQ=255)                                     agagTTTGCTTTTATCAGCAAATGCATCCTGAACACCAGCGATCAGCTGa        >  1:77885/1‑50 (MQ=255)                                      gagTTTGCTTTTATCAGCAAATGCATCCTGAACACCAGCGATCAGCTGAt       <  1:1500441/50‑1 (MQ=255)                                       agTTTGCTTTTATCAGCAAATGCATCCGGAACACCAGCGATCAGCTGATc      <  1:1151961/50‑1 (MQ=38)                                        gTTTGCTTTTATCAGCAAATGCATCCTGAACACCAGCGATCAGCTGATCt     <  1:1443013/50‑1 (MQ=255)                                           tGCTTTTATCAGCAAATGCATCCTGAACACCAGCGATCAGCTGATCttt   <  1:2014355/49‑1 (MQ=255)                                           tGCTTTTATCAGCAAATGCATCCTGAACACCAGCGATCAGCTGATCttt   <  1:1151314/49‑1 (MQ=255)                                           tGCTTTTATCAGCAAATGCATCCTGAACACCAGCGATCAGCTGATCTTTa  >  1:649530/1‑50 (MQ=255)                                               |                                              CGAATGCTTGTAGAGTCTGTTCGATCTCTTGGTCGGAGAGTTTGCTCTTATCAGCAAATGCATCCTGAACACCAGCGATCAGCTGATCTTTA  >  NC_000913/3477104‑3477195

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 31 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑