Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	3,513,003	A→G	D212D (GAT→GAC)	trpS ←	tryptophan‑‑tRNA ligase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	3,513,003	0	A	G	100.0%	114.4 / NA	30	D212D (GAT→GAC)	trpS	tryptophan‑‑tRNA ligase
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base G (18/12);  total (18/12)

GGAGTCAGTGACCGCACGTTTGATTTTCTTCACTACCGATTTCGGATCTTCCAGCAGGCCGATAACGTTATTGCGATTATCGTCAGACTTG  >  NC_000913/3512958‑3513048                                              |                                              ggAGTCAGTGACCGCACGTTTGATTTTCTTCACTACCGATTTCGGGTCtt                                           >  1:1319227/1‑50 (MQ=255) ggAGTCAGTGACCGCACGTTTGATTTTCTTCACTACCGATTTCGGGTCtt                                           <  1:727375/50‑1 (MQ=255) ggAGTCAGTGACCGCACGTTTGATTTTCTTCACTACCGATTTCGGGTCtt                                           >  1:439667/1‑50 (MQ=255) ggAGTCAGTGACCGCACGTTTGATTTTCTTCACTACCGATTTCGGGTCtt                                           >  1:1874402/1‑50 (MQ=255)   aGTCAGTGACCGCACGTTTGATTTTCTTCACTACCGATTTCGGGTCTTcc                                         <  1:2249708/50‑1 (MQ=255)      cAGTGACCGCACGTTTGATTTTCTTCACTACCGATTTCTGGTCTTCcag                                       >  1:1279326/1‑49 (MQ=39)       aGTGACCGCACGTTTGATTTTCTTCACTACCGATTTCGGGTCTTCcagca                                     <  1:1013096/50‑1 (MQ=255)         tGACCGCACGTTTGATTTTCTTCACTACCGATTTCGGGTCTTCCAGCAgg                                   <  1:771585/50‑1 (MQ=255)          gACCGCACGTTTGATTTTCTTCACTACCGATTTCGGGTCTTCCAGCAGGc                                  >  1:784489/1‑50 (MQ=255)              gCACGTTTGATTTTCTTCACTACCGATTTCGGGTCTTCCAGCAGGCCGa                               >  1:56956/1‑49 (MQ=255)               cACGTTTGATTTTCTTCACTACCGATTTCGGGTCTTCCAGCAGGCCGATa                             <  1:212403/50‑1 (MQ=255)                aCGTTTGATTTTCTTCACTACCGATTTCGGGTCTTCCAGCAGGCCGATaa                            <  1:310830/50‑1 (MQ=255)                 cGTTTGATTTTCTTCACTACCGATTTCGGGTCTTCCAGCAGGCCGATAAc                           >  1:1136267/1‑50 (MQ=255)                 cGTTTGATTTTCTTCACTACCGATTTCGGGTCTTCCAGCAGGCCGATAAc                           >  1:2039962/1‑50 (MQ=255)                  gTTTGATTTTCTTCACTACCGATTTCGGGTCTTCCAGCAGGCCGATAACg                          >  1:124344/1‑50 (MQ=255)                  gTTTGATTTTCTTCACTACCGATTTCGGGTCTTCCAGCAGGCCGATAACg                          <  1:82915/50‑1 (MQ=255)                   tttGATTTTCTTCACTACCGATTTCGGGTCTTCCAGCAGGCCGATAACGt                         >  1:2227569/1‑50 (MQ=255)                     tGATTTTCTTCACTACCGATTTCGGGTCTTCCAGCAGGCCGATAACGTTa                       >  1:1885848/1‑50 (MQ=255)                      gATTTTCTTCACTACCGATTTCGGGTCTTCCAGCAGGCCGATAACGTTAt                      >  1:32867/1‑50 (MQ=255)                          ttcttcACTACCGATTTCGGGTCTTCCAGCAGGCCGATAACGTTATTGc                   >  1:27656/1‑49 (MQ=255)                           tcttcACTACCGATTTCGGGTCTTCCAGCAGGCCGATAACGTTATTGCGa                 >  1:1795787/1‑50 (MQ=255)                           tcttcACTACCGATTTCGGGTCTTCCAGCAGGCCGATAACGTTATTGCGa                 >  1:1373922/1‑50 (MQ=255)                               cACTACCGATTTCGGGTCTTCCAGCAGGCCGATAACGTTATTGCGAt                <  1:1762348/47‑1 (MQ=255)                               cACTACCGATTTCGGGTCTTCCAGCAGGCCGATAACGTTATTGCGAt                <  1:8297/47‑1 (MQ=255)                               cACTACCGATTTCGGGTCTTCCAGCAGGCCGATAACGTTATTGCGATTAt             >  1:2022577/1‑50 (MQ=255)                                aCTACCGATTTCGGGTCTTCCAGCAGGCCGATAACGTTATTGCGATTAt             <  1:818124/49‑1 (MQ=255)                                aCTACCGATTTCGGGTCTTCCAGCAGGCCGATAACGTTATTGCGATTATc            <  1:1908672/50‑1 (MQ=255)                                      gATTTCGGGTCTTCCAGCAGGCCGATAACGTTATTGCGATTATCGTCAGa      >  1:298324/1‑50 (MQ=255)                                       aTTTCGGGTCTTCCAGCAGGCCGATAACGTTATTGCGATTATCGTCAGAc     <  1:1513590/50‑1 (MQ=255)                                           cGGGTCTTCCAGCAGGCCGATAACGTTATTGCGATTATCGTCAGACTTg  >  1:1447269/1‑49 (MQ=255)                                              |                                              GGAGTCAGTGACCGCACGTTTGATTTTCTTCACTACCGATTTCGGATCTTCCAGCAGGCCGATAACGTTATTGCGATTATCGTCAGACTTG  >  NC_000913/3512958‑3513048

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑