Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	3,829,233	C→A	L97M (CTG→ATG)	xanP →	xanthine:H(+) symporter XanP

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	3,829,233	0	C	A	100.0%	104.9 / NA	27	L97M (CTG→ATG)	xanP	xanthine:H(+) symporter XanP
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base A (11/16);  total (11/16)

TGGCATCGATTATTCAAATTAAGGCCTGGGGTCCGGTTGGCTCCGGGCTGTTGTCTATTCAGGGCACCAGCTTCAACTTTGTTGCCCCGCTGAT  >  NC_000913/3829186‑3829279                                                |                                               tgGCATCGATTATTCAAATTAAGGCCTGGGGTCCGGTTGGCTCCGGGAtg                                              <  1:1112637/50‑1 (MQ=255)        gATTATTCAAATTAAGGCCTGGGGTCCGGTTGGCTCCGGGATGTTGTCt                                        <  1:1426871/49‑1 (MQ=255)         attattCAAATTAAGGCCTGGGGTCCGGTTGGCTCCGGGATGTTGTCTAt                                      >  1:361416/1‑50 (MQ=255)         attattCAAATTAAGGCCTGGGGTCCGGTTGGCTCCGGGATGTTGTCTAt                                      >  1:139404/1‑50 (MQ=255)         attattCAAATTAAGGCCTGGGGTCCGGTTGGCTCCGGGATGTTGTCTAt                                      <  1:186246/50‑1 (MQ=255)            attCAAATTAAGGCCTGGGGTCCGGTTGGCTCCGGGATGTTGTCTATTc                                    <  1:373806/49‑1 (MQ=255)            attCAAATTAAGGCCTGGGGTCCGGTTGGCTCCGGGATGTTGTCTATTc                                    <  1:2164404/49‑1 (MQ=255)             ttCAAATTAAGGCCTGGGGTCCGGTTGGCTCCGGGATGTTGTCTATTCa                                   <  1:1116096/49‑1 (MQ=255)                   ttAAGGCCTGGGGTCCGGTTGGCTCCGGGATGTTGTCTATTCAGGGCAcc                            <  1:1554889/50‑1 (MQ=255)                    tAAGGCCTGGGGTCCGGTTGGCTCCGGGATGTTGTCTATTCAGGGCAc                             >  1:366209/1‑48 (MQ=255)                     aaGGCCTGGGGTCCGGTTGGCTCCGGGATGTTGTCTATTCAGGGCACCa                           <  1:1522669/49‑1 (MQ=255)                     aaGGCCTGGGGTCCGGTTGGCTCCGGGATGTTGTCTATTCAGGGCACCa                           <  1:1478080/49‑1 (MQ=255)                     aaGGCCTGGGGTCCGGTTGGCTCCGGGATGTTGTCTATTCAGGGCACCAg                          >  1:1357011/1‑50 (MQ=255)                      aGGCCTGGGGTCCGGTTGGCTCCGGGATGTTGTCTATTCAGGGCACCAg                          <  1:2010401/49‑1 (MQ=255)                        gCCTGGGGTCCGGTTGGCTCCGGGATGTTGTCTATTCAGGGCACCAGCtt                       >  1:1298772/1‑50 (MQ=255)                         ccTGGGGTCCGGTTGGCTCCGGGATGTTGTCTATTCAGGGCACCAGCTTc                      <  1:2174823/50‑1 (MQ=255)                          cTGGGGTCCGGTTGGCTCCGGGATGTTGTCTATTCAGGGCACCAGCTTc                      >  1:1938770/1‑49 (MQ=255)                           tGGGGTCCGGTTGGCTCCGGGATGTTGTCTATTCAGGGCACCAGCTTCaa                    >  1:695865/1‑50 (MQ=255)                             gggTCCGGTTGGCTCCGGGATGTTGTCTATTCAGGGCACCAGCTTCAAc                   <  1:305516/49‑1 (MQ=255)                              ggTCCGGTTGGCTCCGGGATGTTGTCTATTCAGGGCACCAGCTTCAACtt                 >  1:529096/1‑50 (MQ=255)                                tCCGGTTGGCTCCGGGATGTTGTCTATTCAGGGCACCAGCTTCAACTttg               <  1:147573/50‑1 (MQ=255)                                    gTTGGCTCCGGGATGTTGTCTATTCAGGGCACCAGCTTCAACTTTGTTGc           >  1:1643264/1‑50 (MQ=255)                                     ttGGCTCCGGGATGTTGTCTATTCAGGGCACCAGCTTCAACTTTGTTGcc          <  1:2211141/50‑1 (MQ=255)                                      tGGCTCCGGGATGTTGTCTATTCAGGGCACCAGCTTCAACTTTGTTGccc         >  1:1849838/1‑50 (MQ=255)                                        gCTCCGGGATGTTGTCTATTCAGGGCACCAGCTTCAACTTTGTTGcccc        <  1:809537/49‑1 (MQ=255)                                            cGGGATGTTGTCTATTCAGGGCACCAGCTTCAACTTTGTTGCCCCGCTGa   >  1:223071/1‑50 (MQ=255)                                             gggATGTTGTCTATTCAGGGCACCAGCTTCAACTTTGTTGCCCCGCTGAt  <  1:1334639/50‑1 (MQ=255)                                                |                                               TGGCATCGATTATTCAAATTAAGGCCTGGGGTCCGGTTGGCTCCGGGCTGTTGTCTATTCAGGGCACCAGCTTCAACTTTGTTGCCCCGCTGAT  >  NC_000913/3829186‑3829279

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑