Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	3,850,891	G→A	Q68* (CAA→TAA)	ilvN ←	acetohydroxy acid synthase I subunit IlvN

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	3,850,891	0	G	A	100.0%	88.2 / NA	23	Q68* (CAA→TAA)	ilvN	acetohydroxy acid synthase I subunit IlvN
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base A (12/11);  total (12/11)

TGATTACGCTGCACTTTCACGACATCTTCCAGCTTATCGATTTGGCTTATCATCTGCTCCAGACGCTGGTCGTCATTGACCAGTAGC  >  NC_000913/3850848‑3850934                                            |                                            tGATTACGCTGCACTTTCACGACATCTTCCAGCTTATCGATTTAGCTTat                                       >  1:486144/1‑50 (MQ=255)   aTTACGCTGCACTTTCACGACATCTTCCAGCTTATCGATTTAGCTTatca                                     <  1:569897/50‑1 (MQ=255)   aTTACGCTGCACTTTCACGACATCTTCCAGCTTATCGATTTAGCTTatca                                     <  1:260894/50‑1 (MQ=255)   aTTACGCTGCACTTTCACGACATCTTCCAGCTTATCGATTTAGCTTatca                                     >  1:316736/1‑50 (MQ=255)   aTTACGCTGCACTTTCACGACATCTTCCAGCTTATCGATTTAGCTTat                                       <  1:1430829/48‑1 (MQ=255)   aTTACGCTGCACTTTCACGACATCTTCCAGCTTATCGATTTAGCTTat                                       <  1:1907866/48‑1 (MQ=255)     tACGCTGCACTTTCACGACATCTTCCAGCTTATCGATTTAGCTTatcatc                                   >  1:446363/1‑50 (MQ=255)          tGCACTTTCACGACATCTTCCAGCTTATCGATTTAGCTTATCATCTGCTc                              >  1:1572508/1‑50 (MQ=255)            cACTTTCACGACATCTTCCAGCTTATCGATTTAGCTTATCATCTGCTcc                             <  1:1053038/49‑1 (MQ=255)            cACTTTCACGACATCTTCCAGCTTATCGATTTAGCTTATCATCTGCTCCa                            <  1:960873/50‑1 (MQ=255)             aCTTTCACGACATCTTCCAGCTTATCGATTTAGCTTATCATCTGCTCCAg                           <  1:1225667/50‑1 (MQ=255)                 tCACGACATCTTCCAGCTTATCGATTTAGCTTATCATCTGCTCCAGACGc                       >  1:1141059/1‑50 (MQ=255)                  cACGACATCTTCCAGCTTATCGATTTAGCTTATCATCTGCTCCAGACGc                       <  1:897059/49‑1 (MQ=255)                  cACGACATCTTCCAGCTTATCGATTTAGCTTATCATCTGCTCCAGACGc                       <  1:1842209/49‑1 (MQ=255)                   aCGACATCTTCCAGCTTATCGTTTTAGCTTATCATCTGCTCCAGACGCTg                     >  1:695381/1‑50 (MQ=255)                   aCGACATCTTCCAGCTTATCGATTTAGCTTATCATCTGCTCCAGACGCt                      <  1:424950/49‑1 (MQ=255)                     gACATCTTCCAGCTTATCGATTTAGCTTATCATCTGCTCCAGACGCTGgt                   >  1:1923826/1‑50 (MQ=255)                                 cTTATCGATTTAGCTTATCATCTGCTCCAGACGCTGGTCGTCATTGAc         >  1:974948/1‑48 (MQ=255)                                  ttATCGATTTAGCTTATCATCTGCTCCAGACGCTGGTCGTCATTGACCAg      >  1:1970544/1‑50 (MQ=255)                                   tATCGATTTAGCTTATCATCTGCTCCAGACGCTGGTCGTCATTGACCAg      >  1:2199650/1‑49 (MQ=255)                                   tATCGATTTAGCTTATCATCTGCTCCAGACGCTGGTCGTCATTGACCAg      >  1:709086/1‑49 (MQ=255)                                     tCGATTTAGCTTATCATCTGCTCCAGACGCTGGTCGTCATTGACCAGTa    <  1:391217/49‑1 (MQ=255)                                         tttAGCTTATCATCTGCTCCAGACGCTGGTCGTCATTGACCAGTAGc  >  1:708695/1‑47 (MQ=255)                                            |                                            TGATTACGCTGCACTTTCACGACATCTTCCAGCTTATCGATTTGGCTTATCATCTGCTCCAGACGCTGGTCGTCATTGACCAGTAGC  >  NC_000913/3850848‑3850934

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑