Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	3,926,696	C→T	G103D (GGC→GAC)	asnC ←	DNA‑binding transcriptional dual regulator AsnC

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	3,926,696	0	C	T	100.0%	110.5 / NA	28	G103D (GGC→GAC)	asnC	DNA‑binding transcriptional dual regulator AsnC
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base T (12/16);  total (12/16)

GAGCGTCGATCGAACGGCACATCACTTTTATAAAGATGCTGTAGTGGCCGGTTGTGTAATAGGCTTCAGTGACTTCATCAAGGCTTTCCAGCTT  >  NC_000913/3926649‑3926742                                                |                                               gagCGTCGATCGAACGGCACATCACTTTTATAAAGATGCTGTAGTGGTCg                                              >  1:1666438/1‑50 (MQ=255)   gCGTCGATCGAACGGCACATCACTTTTATAAAGATGCTGTAGTGGTCGGt                                            <  1:1293599/50‑1 (MQ=255)     gTCGATCGAACGGCACATCACTTTTATAAAGATGCTGTAGTGGTCGGTtg                                          <  1:1461150/50‑1 (MQ=255)       cgatcgaACGGCACATCACTTTTATAAAGATGCTGTAGTGGTCGGTtgtg                                        >  1:1747363/1‑50 (MQ=255)        gatcgaACGGCACATCACTTTTATAAAGATGCTGTAGTGGTCGGTTgtgt                                       <  1:181268/50‑1 (MQ=255)         atcgaACGGCACATCACTTTTATAAAGATGCTGTAGTGGTCGGTTGTGTa                                      <  1:1431968/50‑1 (MQ=255)          tcgaACGGCACATCACTTTTATAAAGATGCTGTAGTGGTCGGTTGTGTaa                                     <  1:1413626/50‑1 (MQ=255)            gaACGGCACATCACTTTTATAAAGATGCTGTAGTGGTCGGTTGTGTAATa                                   <  1:176400/50‑1 (MQ=255)                ggCACATCACTTTTATAAAGATGCTGTAGTGGTCGGTTGTGTAATAGGCt                               >  1:532061/1‑50 (MQ=255)                 gCACATCACTTTTATAAAGATGCTGTAGTGGTCGGTTGTGTAATAGGCtt                              <  1:1337518/50‑1 (MQ=255)                 gCACATCACTTTTATAAAGATGCTGTAGTGGTCGGTTGTGTAATAGGCtt                              >  1:1819040/1‑50 (MQ=255)                  cacaTCACTTTTATAAAGATGCTGTAGTGGTCGGTTGTGTAATAGGCtt                              >  1:1569555/1‑49 (MQ=255)                  cacaTCACTTTTATAAAGATGCTGTAGTGGTCGGTTGTGTAATAGGCTTc                             <  1:1071815/50‑1 (MQ=255)                        aCTTTTATAAAGATGCTGTAGCGGTCGGTTGTGTAACAGGCTTCAGTGAc                       >  1:34006/1‑50 (MQ=255)                         cTTTTATAAAGATGCTGTAGTGGTCGGTTGTGTAATAGGCTTCAGTGACt                      >  1:735722/1‑50 (MQ=255)                          ttttATAAAGATGCTGTAGTGGTCGGTTGTGTAATAGGCTTCAGTGACtt                     >  1:572312/1‑50 (MQ=255)                            ttATAAAGATGCTGTAGTGGTCGGTTGTGTAATAGGCTTCAGTGACtt                     <  1:403160/48‑1 (MQ=255)                            ttATAAAGATGCTGTAGTGGTCGGTTGTGTAATAGGCTTCAGTGACTtc                    <  1:476582/49‑1 (MQ=255)                             tataAAGATGCTGTAGTGGTCGGTTGTGTAATAGGCTTCAGTGACTtcat                  >  1:178804/1‑50 (MQ=255)                                  aGATGCTGTAGTGGTCGGTTGTGTAATAGGCTTCAGTGACTTCATCAAgg             >  1:2078152/1‑50 (MQ=255)                                  aGATGCTGTAGTGGTCGGTTGTGTAATAGGCTTCAGTGACTTCATCAAgg             <  1:730025/50‑1 (MQ=255)                                   gATGCTGTAGTGGTCGGTTGTGTAATAGGCTTCAGTGACTTCATCAAGGc            >  1:2074517/1‑50 (MQ=255)                                       cTGTAGTGGTCGGTTGTGTAATAGGCTTCAGTGACTTCATCAAGGCttt         <  1:1747207/49‑1 (MQ=255)                                       cTGTAGTGGTCGGTTGTGTAATAGGCTTCAGTGACTTCATCAAGGCttt         <  1:53045/49‑1 (MQ=255)                                          tAGTGGTCGGTTGTGTAATAGGCTTCAGTGACTTCATCAAGGCTTTCCAg     <  1:1861070/50‑1 (MQ=255)                                            gTGGTCGGTTGTGTAATAGGCTTCAGTGACTTCATCAAGGCTTTCCAGCt   >  1:366484/1‑50 (MQ=255)                                            gTGGTCGGTTGTGTAATAGGCTTCAGTGACTTCATCAAGGCTTTCCAGCt   <  1:1456900/50‑1 (MQ=255)                                             tGGTCGGTTGTGTAATAGGCTTCAGTGACTTCATCAAGGCTTTCCAGCtt  <  1:1196129/50‑1 (MQ=255)                                                |                                               GAGCGTCGATCGAACGGCACATCACTTTTATAAAGATGCTGTAGTGGCCGGTTGTGTAATAGGCTTCAGTGACTTCATCAAGGCTTTCCAGCTT  >  NC_000913/3926649‑3926742

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 30 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑