Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	3,979,769	C→T	A390V (GCG→GTG)	wzyE →	enterobacterial common antigen polymerase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	3,979,769	0	C	T	100.0%	105.5 / NA	27	A390V (GCG→GTG)	wzyE	enterobacterial common antigen polymerase
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base T (12/15);  total (12/15)

GCCTAATCGCTATAAAGCTGCGATATTGCACAGTTTCTGCTTTGGGGCGATCTTCAATATGATCGTGCTGGCGCGTGAAGGGCTGGATTCGTTT  >  NC_000913/3979722‑3979815                                                |                                               gCCTAATCGCTATAAAGCTGCGATATTGCACAGTTTCTGCTTTGGGGTGa                                              <  1:1424234/50‑1 (MQ=255)    tAATCGCTATAAAGCTGCGATATTGCACAGTTTCTGCTTTGGGGTGATCt                                           >  1:2011218/1‑50 (MQ=255)       tCGCTATAAAGCTGCGATATTGCACAGTTTCTGCTTTGGGGTGATCTTCa                                        <  1:1102651/50‑1 (MQ=255)         gCTATAAAGCTGCGATATTGCACAGTTTCTGCTTTGGGGTGATCTTCaa                                       <  1:66262/49‑1 (MQ=255)         gCTATAAAGCTGCGATATTGCACAGTTTCTGCTTTGGGGTGATCTTCaa                                       <  1:612028/49‑1 (MQ=255)          cTATAAAGCTGCGATATTGCACAGTTTCTGCTTTGGGGTGATCTTCAata                                     <  1:116512/50‑1 (MQ=255)          cTATAAAGCTGCGATATTGCACAGTTTCTGCTTTGGGGTGATCTTCAata                                     >  1:1562280/1‑50 (MQ=255)             taAAGCTGCGATATTGCACAGTTTCTGCTTTGGGGTGATCTTCAatat                                    <  1:1499123/48‑1 (MQ=255)             taAAGCTGCGATATTGCACAGTTTCTGCTTTGGGGTGATCTTCAatat                                    <  1:244080/48‑1 (MQ=255)              aaaGCTGCGATATTGCACAGTTTCTGCTTTGGGGTGATCTTCAATATGAt                                 >  1:2071188/1‑50 (MQ=255)               aaGCTGCGATATTGCACAGTTTCTGCTTTGGGGTGATCTTCAATATGATc                                >  1:680002/1‑50 (MQ=255)                 gCTGCGATATTGCACAGTTTCTGCTTTGGGGTGATCTTCAATATGATCGt                              <  1:1681245/50‑1 (MQ=255)                     cGATATTGCACAGTTTCTGCTTTGGGGTGATCTTCAATATGATCGTGc                            <  1:2252762/48‑1 (MQ=255)                     cGATATTGCACAGTTTCTGCTTTGGGGTGATCTTCAATATGATCGTGCt                           <  1:741524/49‑1 (MQ=255)                       atatTGCACAGTTTCTGCTTTGGGGTGATCTTCAATATGATCGTGCTGgc                        >  1:493348/1‑50 (MQ=255)                          ttGCACAGTTTCTGCTTTGGGGTGATCTTCAATATGATCGTGCTGGcgcg                     >  1:1665445/1‑50 (MQ=255)                              acaGTTTCTGCTTTGGGGTGATCTTCAATATGATCGTGCTGGCGCGTg                   >  1:715430/1‑48 (MQ=255)                                aGTTTCTGCTTTGGGGTGATCTTCAATATGATCGTGCTGGCGCGTGAAgg               >  1:2057376/1‑50 (MQ=255)                                    tCTGCTTTGGGGTGATCTTCAATATGATCGTGCTGGCGCGTGAAGGGCt            <  1:1858219/49‑1 (MQ=255)                                       gCTTTGGGGTGATCTTCAATATGATCGTGCTGGCGCGTGAAGGGCTGGAt        >  1:595300/1‑50 (MQ=255)                                        cTTTGGGGTGATCTTCAATATGATCGTGCTGGCGCGTGAAGGGCTgg          <  1:179/47‑1 (MQ=255)                                        cTTTGGGGTGATCTTCAATATGATCGTGCTGGCGCGTGAAGGGCTgg          <  1:2078585/47‑1 (MQ=255)                                        cTTTGGGGTGATCTTCAATATGATCGTGCTGGCGCGTGAAGGGCTGGAtt       >  1:1250271/1‑50 (MQ=255)                                           tGGGGTGATCTTCAATATGATCGTGCTGGCGCGTGAAGGGCTGGATTc      <  1:400767/48‑1 (MQ=255)                                            ggggTGATCTTCAATATGATCGTGCTGGCGCGTGAAGGGCTGGATTCGtt   >  1:1416045/1‑50 (MQ=255)                                            ggggTGATCTTCAATATGATCGTGCTGGCGCGTGAAGGGCTGGATTCGt    <  1:416740/49‑1 (MQ=255)                                             gggTGATCTTCAATATGATCGTGCTGGCGCGTGAAGGGCTGGATTCGttt  >  1:2088432/1‑50 (MQ=255)                                                |                                               GCCTAATCGCTATAAAGCTGCGATATTGCACAGTTTCTGCTTTGGGGCGATCTTCAATATGATCGTGCTGGCGCGTGAAGGGCTGGATTCGTTT  >  NC_000913/3979722‑3979815

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑