Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	380,022	(G)10→9	intergenic (‑141/+47)	frmR ← / ← yaiO	DNA‑binding transcriptional repressor FrmR/outer membrane protein YaiO

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	380,013	0	G	.	100.0%	117.1 / NA	25	intergenic (‑132/+56)	frmR/yaiO	DNA‑binding transcriptional repressor FrmR/outer membrane protein YaiO
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base . (16/9);  total (16/9)

TGGAGAATAAAATTTATCCGGTGAATGTGGTCGGAAAACAAAGAGGAAAGGGGGGGGGGCTAATCGGCAGGGAAGGCCGCCCCGGATAGCGGGC  >  NC_000913/379964‑380057                                                  |                                             tGGAGAATAAAATTTATCCGGTGAATGTGGTCGGAAAACAAAGAGGAAAg                                              >  1:535631/1‑50 (MQ=255) tGGAGAATAAAATTTATCCGGTGAATGTGGTCGGAAAACAAAGAGGAAAg                                              >  1:1600756/1‑50 (MQ=255)  ggAGAATAAAATTTATCCGGTGAATGTGGTCGGAAAACAAAGAGGAAAgg                                             >  1:2632589/1‑50 (MQ=255)      aaTAAAATTTATCCGGTGAATGTGGTCGGAAAACAAAGAGGAAAgggggg                                         >  1:1594711/1‑50 (MQ=255)        tAAAATTTATCCGGTGAATGTGGTCGGAAAACAAAGAGGAAAgggggggg                                       <  1:730880/50‑1 (MQ=255)          aaaTTTATCCGGTGAATGTGGTCGGAAAACAAAGAGGAAAgggggggggc                                     <  1:2507627/50‑2 (MQ=255)          aaaTTTATCCGGTGAATGTGGTCGGAAAACAAAGAGGAAAgggggggggc                                     <  1:1549213/50‑2 (MQ=255)           aaTTTATCCGGTGAATGTGGTCGGAAAACAAAGAGGAAAgggggggggct                                    <  1:2509219/50‑3 (MQ=255)           aaTTTATCCGGTGAATGTGGTCGGAAAACAAAGAGGAAAgggggggggct                                    <  1:2656108/50‑3 (MQ=255)            aTTTATCCGGTGAATGTGGTCGGAAAACAAAGAGGAAAgggggggggct                                    >  1:819803/1‑47 (MQ=255)              ttATCCGGTGAATGTGGTCGGAAAACAAAGAGGAAA‑GGGGGGGGGCTAAt                                >  1:1358599/1‑50 (MQ=255)               tATCCGGTGAATGTGGTCGGAAAACAAAGAGGAAA‑GGGGGGGGGCTAATc                               >  1:1891983/1‑50 (MQ=255)                 tCCGGTGAATGTGGTCGGAAAACAAAGAGGAAA‑GGGGGGGGGCTAATCg                              <  1:1593561/49‑1 (MQ=255)                     gTGAATGTGGTCGGAAAACAAAGAGGAAA‑GGGGGGGGGCTAATCGGCAgg                         <  1:2681694/50‑1 (MQ=255)                     gTGAATGTGGTCGGAAAACAAAGAGGAAA‑GGGGGGGGGCTAATCGGCAgg                         >  1:426401/1‑50 (MQ=255)                      tGAATGTGGTCGGAAAACAAAGAGGAAA‑GGGGGGGGGCTAATCGGCAgg                         >  1:2782220/1‑49 (MQ=255)                        aaTGTGGTCGGAAAACAAAGAGGAAA‑GGGGGGGGGCTAATCGGCAggg                        >  1:861535/1‑48 (MQ=255)                          tgtgGTCGGAAAACAAAGAGGAAA‑GGGGGGGGGCTAATCGGCAGGGAAgg                    >  1:1924481/1‑50 (MQ=255)                          tgtgGTCGGAAAACAAAGAGGAAA‑GGGGGGGGGCTAATCGGCAGGGAAgg                    >  1:2560725/1‑50 (MQ=255)                          tgtgGTCGGAAAACAAAGAGGAAA‑GGGGGGGGGCTAATCGGCAGGGAAgg                    >  1:1168101/1‑50 (MQ=255)                            tgGTCGGAAAACAAAGAGGAAA‑GGGGGGGGGCTAATCGGCAGGGAAGgcc                  <  1:1250348/50‑1 (MQ=255)                              gTCGGAAAACAAAGAGGAAA‑GGGGGGGGGCTAATCGGCAGGGAAGgccg                 <  1:1892271/49‑1 (MQ=255)                                cGGAAAACAAAGAGGAAA‑GGGGGGGGGCTAATCGGCAGGGAAGGCCGccc              >  1:1164044/1‑50 (MQ=255)                                   aaaaCAAAGAGGAAA‑GGGGGGGGGCTAATCGGCAGGGAAGGCCGCCCCg            >  1:1470887/1‑49 (MQ=255)                                      aCAAAGAGGAAA‑GGGGGGGGGCTAATCGGCAGGGAAGGCCGCCCCGGa          >  1:1837871/1‑48 (MQ=255)                                      aCAAAGAGGAAA‑GGGGGGGGGCTAATCGGCAGGGAAGGCCGCCCCGGa          >  1:40069/1‑48 (MQ=255)                                      aCAAAGAGGAAA‑GGGGGGGGGCTAATCGGCAGGGAAGGCCGCCCCGGATa        <  1:2380185/50‑1 (MQ=255)                                      aCAAAGAGGAAA‑GGGGGGGGGCTAATCGGCAGGGAAGGCCGCCCCGGATa        <  1:768215/50‑1 (MQ=255)                                       cAAAGAGGAAA‑GGGGGGGGGCTAATCGGCAGGGAAGGCCGCCCCGGATAg       <  1:11650/50‑1 (MQ=255)                                        aaaGAGGAAA‑GGGGGGGGGCTAATCGGCAGGGAAGGCCGCCCCGGATAgc      >  1:180324/1‑50 (MQ=255)                                         aaGAGGAAA‑GGGGGGGGGCTAATCGGCAGGGAAGGCCGCCCCGGATAgcg     >  1:2074717/1‑50 (MQ=255)                                         aaGAGGAAA‑GGGGGGGGGCTAATCGGCAGGGAAGGCCGCCCCGGATAgcg     >  1:1782317/1‑50 (MQ=255)                                         aaGAGGAAA‑GGGGGGGGGCTAATCGGCAGGGAAGGCCGCCCCGGATAgcg     <  1:1243756/50‑1 (MQ=255)                                          agagGAAA‑GGGGGGGGGCTAATCGGCAGGGAAGGCCGCCCCGGATAgcgg    >  1:1266504/1‑50 (MQ=255)                                            agGAAA‑GGGGGGGGGCTAATCGGCAGGGAAGGCCGCCCCGGATAgcgggc  <  1:672249/50‑1 (MQ=255)                                                  |                                             TGGAGAATAAAATTTATCCGGTGAATGTGGTCGGAAAACAAAGAGGAAAGGGGGGGGGGCTAATCGGCAGGGAAGGCCGCCCCGGATAGCGGGC  >  NC_000913/379964‑380057

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 29 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑