Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	765,191	Δ1 bp	coding (685/723 nt)	mngR ←	DNA‑binding transcriptional repressor MngR

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	765,189	0	T	.	100.0%	189.2 / NA	40	coding (687/723 nt)	mngR	DNA‑binding transcriptional repressor MngR
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base . (24/16);  total (24/16)

GCCTTTTTTGTGCTTATCGCGATGATTTTCGCTGCGCTATCAGGGTAAATTTATAGTCATCGGTATTAAAAGCGTTGCGGCTATATTCAAACACC  >  NC_000913/765140‑765234                                                  |                                              gCCTTTTTTGTGCTTATCGCGATGATTTTCGCTGCGCTATCAGGGTAAAt                                               <  1:2412143/50‑1 (MQ=255)  ccTTTTTTGTGCTTATCGCGATGATTTTCGCTGCGCTATCAGGGTAAAt                                               >  1:1873396/1‑49 (MQ=255)      ttttGTGCTTATCGCGATGATTTTCGCTGCGCTATCAGGGTAAAttat                                            <  1:1569483/48‑3 (MQ=255)         tgtgCTTATCGCGATGATTTTCGCTGCGCTATCAGGGTAAA‑TTATAGTCa                                      >  1:641599/1‑50 (MQ=39)         tgtgCTTATCGCGATGATTTTCGCTGCGCTATCAGGGTAAA‑TTATAGTCa                                      >  1:1258570/1‑50 (MQ=39)         tgtgCTTATCGCGATGATTTTCGCTGCGCTATCAGGGTAAA‑TTATAGTCa                                      >  1:444930/1‑50 (MQ=39)          gtgCTTATCGCGATGATTTTCGCTGCGCTATCAGGGTAAA‑TTATAGTCAt                                     >  1:1784440/1‑50 (MQ=39)           tgCTTATCGCGATGATTTTCGCTGCGCTATCAGGGTAAA‑TTATAGTCATc                                    <  1:1163356/50‑1 (MQ=39)            gCTTATCGCGATGATTTTCGCTGCGCTATCAGGGTAAA‑TTATAGTCATCg                                   >  1:1497425/1‑50 (MQ=39)              ttATCGCGATGATTTTCGCTGCGCTATCAGGGTAAA‑TTATAGTCATCgg                                  >  1:592110/1‑49 (MQ=38)               tATCGCGATGATTTTCGCTGCGCTATCAGGGTAAA‑TTATAGTCATCGGTa                                >  1:685319/1‑50 (MQ=39)                   gcgATGATTTTCGCTGCGCTATCAGGGTAAA‑TTATAGTCATCGGTATTA                             >  1:2440995‑M1/1‑49 (MQ=255)                    cgATGATTTTCGCTGCGCTATCAGGGTAAA‑TTATAGTCATCGGTATTAta                           <  1:464902‑M1/50‑3 (MQ=255)                    cgATGATTTTCGCTGCGCTATCAGGGTAAA‑TTATAGTCATCGGTATTAta                           <  1:2201977‑M1/50‑3 (MQ=255)                     gatgatTTTCGCTGCGCTATCAGGGTAAA‑TTATAGTCATCGGTATTAtag                          >  1:1421030‑M1/1‑47 (MQ=255)                      atgatTTTCGCTGCGCTATCAGGGTAAA‑TTATAGTCATCGGTATTAtag                          >  1:1971360‑M1/1‑46 (MQ=255)                       tgatTTTCGCTGCGCTATCAGGGTAAA‑TTATAGTCATCGGTATTAtagca                        <  1:357254‑M1/50‑6 (MQ=255)                        gatTTTCGCTGCGCTATCAGGGTAAA‑TTATAGTCATCGGTATTAtagcaa                       >  1:723435‑M1/1‑44 (MQ=255)                         atTTTCGCTGCGCTATCAGGGTAAA‑TTATAGTCATCGGTATTAtagcaat                      <  1:977094‑M1/50‑8 (MQ=255)                         atTTTCGCTGCGCTATCAGGGTAAA‑TTATAGTCATCGGTATTAtagcaat                      >  1:1835466‑M1/1‑43 (MQ=255)                          ttttCGCTGCGCTATCAGGGTAAA‑TTATAGTCATCGGTATTAtagcaatc                     <  1:1889011‑M1/50‑9 (MQ=255)                           tttCGCTGCGCTATCAGGGTAAA‑TTATAGTCATCGGTATTAtagcaatc                     >  1:572842‑M1/1‑41 (MQ=255)                             tCGCTGCGCTATCAGGGTAAA‑TTATAGTCATCGGTATTAtagcaatcaaa                  >  1:1551522‑M1/1‑39 (MQ=255)                              cGCTGCGCTATCAGGGTAAA‑TTATAGTCATCGGTATTAtagcaatcaaac                 >  1:2102053‑M1/1‑38 (MQ=255)                              cGCTGCGCTATCAGGGTAAA‑TTATAGTCATCGGTATTAtagcaatcaaac                 >  1:691464‑M1/1‑38 (MQ=255)                               gCTGCGCTATCAGGGTAAA‑TTATAGTCATCGGTATTAtagcaatcaaacc                <  1:2306536‑M1/50‑14 (MQ=255)                               gCTGCGCTATCAGGGTAAA‑TTATAGTCATCGGTATTAtagcaatcaaac                 <  1:1268418‑M1/49‑13 (MQ=255)                               gCTGCGCTATCAGGGTAAA‑TTATAGTCATCGGTATTAtagcaatcaaac                 <  1:893664‑M1/49‑13 (MQ=255)                               gCTGCGCTATCAGGGTAAA‑TTATAGTCATCGGTATTAtagcaatcaaac                 <  1:1710535‑M1/49‑13 (MQ=255)                                cTGCGCTATCAGGGTAAA‑TTATAGTCATCGGTATTAtagcaatcaaaccg               <  1:1280531‑M1/50‑15 (MQ=255)                                cTGCGCTATCAGGGTAAA‑TTATAGTCATCGGTATTAtagcaatcaaaccg               <  1:1509561‑M1/50‑15 (MQ=255)                                cTGCGCTATCAGGGTAAA‑TTATAGTCATCGGTATTAtagcaatcaaacc                >  1:99299‑M1/1‑36 (MQ=255)                                   cgcTATCAGGGTAAA‑TTATAGTCATCGGTATTAtagcaatcaaaccgaag            >  1:2364944‑M1/1‑33 (MQ=255)                                    gcTATCAGGGTAAA‑TTATAGTCATCGGTATTAtagcaatcaaaccgaagc           >  1:2306939‑M1/1‑32 (MQ=255)                                       aTCAGGGTAAA‑TTATAGTCATCGGTATTAtagcaatcaaaccgaagcca         >  1:1083799‑M1/1‑29 (MQ=255)                                       aTCAGGGTAAA‑TTATAGTCATCGGTATTAtagcaatcaaaccgaagcca         >  1:1353472‑M1/1‑29 (MQ=255)                                       aTCAGGGTAAA‑TTATAGTCATCGGTATTAtagcaatcaaaccgaagcca         >  1:1276812‑M1/1‑29 (MQ=255)                                        tCAGGGTAAA‑TTATAGTCATCGGTATTAtagcaatcaaaccgaagccaca       <  1:2481719‑M1/50‑23 (MQ=255)                                        tCAGGGTAAA‑TTATAGTCATCGGTATTAtagcaatcaaaccgaagccac        <  1:766286‑M1/49‑22 (MQ=255)                                        tCAGGGTAAA‑TTATAGTCATCGGTATTAtagcaatcaaaccgaagcca         <  1:1715551‑M1/48‑21 (MQ=255)                                            ggTAAA‑TTATAGTCATCGGTATTAtagcaatcaaaccgaagccacatatg   >  1:2533583‑M1/1‑24 (MQ=255)                                             gTAAA‑TTATAGTCATCGGTATTAtagcaatcaaaccgaagccacatatgc  >  1:1657562‑M1/1‑23 (MQ=255)                                             gTAAA‑TTATAGTCATCGGTATTAtagcaatcaaaccgaagccacatatgc  <  1:1421572‑M1/50‑28 (MQ=255)                                                  |                                              GCCTTTTTTGTGCTTATCGCGATGATTTTCGCTGCGCTATCAGGGTAAATTTATAGTCATCGGTATTAAAAGCGTTGCGGCTATATTCAAACACC  >  NC_000913/765140‑765234

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑