Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	972,866	Δ1 bp	coding (536/780 nt)	elyC ←	envelope biogenesis factor

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	972,863	0	G	.	100.0%	90.8 / NA	22	coding (539/780 nt)	elyC	envelope biogenesis factor
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base . (8/14);  total (8/14)

AAAGATCATCGCCCGCGGCAGATGTGATGCGGAGGTCACCAGCAGGAAAGGGGCGTCACCAATCGCCTGTTTCACTGCTGCAGCTTCTTCTTC  >  NC_000913/972814‑972906                                                  |                                            aaaGATCATCGCCCGCGGCAGATGTGATGCGGAGGTCACCAGCAGGAAAg                                             <  1:339452/50‑1 (MQ=255) aaaGATCATCGCCCGCGGCAGATGTGATGCGGAGGTCACCAGCAGGAAAg                                             >  1:1822824/1‑50 (MQ=255)   aGATCATCGCCCGCGGCAGATGTGATGCGGAGGTCACCAGCAGGAAAggg                                           >  1:1416589/1‑50 (MQ=255)     atcatcGCCCGCGGCAGATGTGATGCGGAGGTCACCAGCAGGAAAgggcg                                         >  1:620811/1‑48 (MQ=255)           gCCCGCGGCAGATGTGATGCGGAGGTCACCAGCAGGAAA‑GGGCGTCAcc                                   <  1:587622/49‑1 (MQ=39)           gCCCGCGGCAGATGTGATGCGGAGGTCACCAGCAGGAAA‑GGGCGTCAcc                                   >  1:2259592/1‑49 (MQ=39)           gCCCGCGGCAGATGTGATGCGGAGGTCACCAGCAGGAAA‑GGGCGTCAcc                                   <  1:2140867/49‑1 (MQ=39)                 ggCAGATGTGATGCGGAGGTCACCAGCAGGAAA‑GGGCGTCACCAATCGcc                            >  1:1459052/1‑50 (MQ=39)                   cAGATGTGATGCGGAGGTCACCAGCAGGAAA‑GGGCGTCACCAATCGCCTg                          >  1:298453/1‑50 (MQ=39)                    aGATGTGATGCGGAGGTCACCAGCAGGAAA‑GGGCGTCACCAATCGCCTGt                         >  1:2049529/1‑50 (MQ=39)                    aGATGTGATGCGGAGGTCACCAGCAGGAAA‑GGGCGTCACCAATCGCCTGt                         >  1:2160546/1‑50 (MQ=39)                     gATGTGATGCGGAGGTCACCAGCAGGAAA‑GGGCGTCACCAATCGCCTGtt                        >  1:296388/1‑50 (MQ=39)                      aTGTGATGCGGAGGTCACCAGCAGGAAA‑GGGCGTCACCAATCGCCTGttt                       <  1:766011/50‑1 (MQ=39)                      aTGTGATGCGGAGGTCACCAGCAGGAAA‑GGGCGTCACCAATCGCCTGttt                       <  1:1359362/50‑1 (MQ=39)                           aTGCGGAGGTCACCAGCAGGAAA‑GGGCGTCACCAATCGCCTGTTTCActg                  <  1:1652639/50‑1 (MQ=39)                           aTGCGGAGGTCACCAGCAGGAAA‑GGGCGTCACCAATCGCCTGTTTCActg                  <  1:405805/50‑1 (MQ=39)                           aTGCGGAGGTCACCAGCAGGAAA‑GGGCGTCACCAATCGCCTGTTTCAct                   <  1:1878394/49‑1 (MQ=39)                           aTGCGGAGGTCACCAGCAGGAAA‑GGGCGTCACCAATCGCCTGTTTCAct                   <  1:1651764/49‑1 (MQ=39)                              cGGAGGTCACCAGCAGGAAA‑GGGCGTCACCAATCGCCTGTTTCActgct                <  1:1015363/49‑1 (MQ=39)                                      aCCAGCAGGAAA‑GGGCGTCACCAATCGCCTGTTTCACTGCTGCAGcttct       <  1:2146114/50‑1 (MQ=39)                                      aCCAGCAGGAAA‑GGGCGTCACCAATCGCCTGTTTCACTGCTGCAGcttct       >  1:2493484/1‑50 (MQ=39)                                      aCCAGCAGGAAA‑GGGCGTCACCAATCGCCTGTTTCACTGCTGCAGcttc        <  1:1258430/49‑1 (MQ=39)                                      aCCAGCAGGAAA‑GGGCGTCACCAATCGCCTGTTTCACTGCTGCAGcttc        <  1:1606963/49‑1 (MQ=39)                                        cagcagGAAA‑GGGCGTCACCAATCGCCTGTTTCACTGCTGCAGcttcttc     >  1:517690/1‑50 (MQ=39)                                          gcagGAAA‑GGGCGTCACCAATCGCCTGTTTCACTGCTGCAGcttcttctt   <  1:1506419/50‑1 (MQ=39)                                           cagGAAA‑GGGCGTCACCAATCGCCTGTTTCACTGCTGCAGCttcttcttc  <  1:831920/50‑1 (MQ=39)                                                  |                                            AAAGATCATCGCCCGCGGCAGATGTGATGCGGAGGTCACCAGCAGGAAAGGGGCGTCACCAATCGCCTGTTTCACTGCTGCAGCTTCTTCTTC  >  NC_000913/972814‑972906

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 31 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑