Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	1,847,724	(C)6→7	coding (285/1044 nt)	selD ←	selenide, water dikinase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	1,847,718	1	.	C	100.0%	62.1 / NA	19	G97G (GGC→GGG)	selD	selenide, water dikinase
Reads supporting (aligned to +/- strand):  ref base . (0/0);  new base C (7/12);  total (7/12)

AAAGTTTGTTAATCGGCCAGCCGAGGATCGCAATCGCCATAATCGGTTTG‑CCCCCCATCGCGAAGATATCGCTGATGGCGTTAGTCGCCGCAA  >  NC_000913/1847669‑1847761                                                   |                                            aaaGTTTGTTAATCGGCCAGCCTAGGATCGCAATCGCCATAATCGGTTTg                                              >  1:1304345/1‑50 (MQ=255)  aaGTTTGTTAATCGGCCAGCCGAGGATCGCAATCGCCATAATCGGTTTG‑c                                            >  1:1800338/1‑50 (MQ=255)     tttGTTAATCGGCCAGCCGAGGATCGCAATCGCCATAATCGGTTTG‑cccc                                         >  1:1370784/1‑50 (MQ=255)       tGTTAATCGGCCAGCCGAGGATCGCAATCGCCATAATCGGTTTG‑cccccc                                       >  1:223728/1‑50 (MQ=255)       tGTTAATCGGCCAGCCGAGGATCGCAATCGCCATAATCGGTTTG‑ccccc                                        <  1:273417/49‑1 (MQ=255)        gTTAATCGGCCAGCCGAGGATCGCAATCGCCATAATCGGTTTG‑cccccc                                       >  1:2331130/1‑49 (MQ=255)         ttAATCGGCCAGCCGAGGATCGCAATCGCCATAATCGGTTTG‑ccccccc                                      <  1:1253707/49‑2 (MQ=255)         ttAATCGGCCAGCCGAGGATCGCAATCGCCATAATCGGTTTG‑ccccccc                                      <  1:2026064/49‑2 (MQ=255)             tCGGCCAGCCGAGGATCGCAATCGCCATAATCGGTTTGCCCCCCCATcgc                                  <  1:1015074/50‑1 (MQ=38)                 ccAGCCGAGGATCGCAATCGCCATAATCGGTTTGCCCCCCCATCGCGAAg                              <  1:962961/50‑1 (MQ=38)                    gCCGAGGATCGCAATCGCCATAATCGGTTTGCCCCCCCATCGCGAAGata                           >  1:1138838/1‑50 (MQ=38)                    gCCGAGGATCGCAATCGCCATAATCGGTTTGCCCCCCCATCGCGAAGata                           >  1:2498654/1‑50 (MQ=38)                     ccGAGGATCGCAATCGCCATAATCGGTTTGCCCCCCCATCGCGAAGatat                          >  1:2091956/1‑50 (MQ=38)                      cGAGGATCGCAATCGCCATAATCGGTTTGCCCCCCCATCGCGAAGATATc                         <  1:2063836/50‑1 (MQ=38)                      cGAGGATCGCAATCGCCATAATCGGTTTGCCCCCCCATCGCGAAGATATc                         >  1:1601411/1‑50 (MQ=38)                       gAGGATCGCAATCGCCATAATCGGTTTGCCCCCCCATCGCGAAGATATCg                        <  1:2662789/50‑1 (MQ=38)                            tCGCAATCGCCATAATCGGTTTGCCCCCCCATCGCGAAGATATCGCTGAt                   >  1:622663/1‑50 (MQ=38)                            tCGCAATCGCCATAATCGGTTTGCCCCCCCATCGCGAAGATATCGCTGAt                   <  1:1078545/50‑1 (MQ=38)                              gCAATCGCCATAATCGGTTTGCCCCCCCATCGCGAAGATATCGCTGATgg                 <  1:1371437/50‑1 (MQ=38)                              gCAATCGCCATAATCGGTTTGCCCCCCCATCGCGAAGATATCGCTGATgg                 >  1:685781/1‑50 (MQ=38)                               cAATCGCCATAATCGGTTTGCCCCCCCATCGCGAAGATATCGCTGATGGc                <  1:1481953/50‑1 (MQ=39)                               cAATCGCCATAATCGGTTTGCCCCCCCATCGCGAAGATATCGCTGATGGc                <  1:2688232/50‑1 (MQ=39)                                     ccATAATCGGTTTGCCCCCCCATCGCGAAGATATCGCTGATGGCGTTAGt          <  1:2747237/50‑1 (MQ=255)                                       aTAATCGGTTTGCCCCCCCATCGCGAAGATATCGCTGATGGCGTTAGTcg        <  1:291881/50‑1 (MQ=255)                                          aTCGGTTTGCCCCCCCATCGCGAAGATATCGCTGATGGCGTTAGTcgccg     <  1:830602/50‑1 (MQ=39)                                          aTCGGTTTGCCCCCCCATCGCGAAGATATCGCTGATGGCGTTAGTcgcc      <  1:2282812/49‑1 (MQ=38)                                             ggTTTGCCCCCCCATCGCGAAGATATCGCTGATGGCGTTAGTCGCCGCaa  >  1:2091854/1‑50 (MQ=39)                                                   |                                            AAAGTTTGTTAATCGGCCAGCCGAGGATCGCAATCGCCATAATCGGTTTG‑CCCCCCATCGCGAAGATATCGCTGATGGCGTTAGTCGCCGCAA  >  NC_000913/1847669‑1847761

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 16 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 35 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑