Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	4,485,906	C→A	A16S (GCC→TCC)	pepA ←	aminopeptidase A/I

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	4,485,906	0	C	A	100.0%	135.5 / NA	34	A16S (GCC→TCC)	pepA	aminopeptidase A/I
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base A (17/17);  total (17/17)

AATCGGAGAAAGGCGACGTGGTTCGAAGACGCCCACGACGATGCAGGCACTCCGCTGTTTCTCCGGGCTACCGCTTTTTACACTAAACTCCATG  >  NC_000913/4485859‑4485952                                                |                                               aaTCGGAGAAAGGCGACGTGGTTCGAAGACGCCCACGACGATGCAGGAAc                                              >  1:2062964/1‑50 (MQ=255)     ggAGAAAGGCGACGTGGTTCGAAGACGCCCACGACGATGCAGGAACTCCg                                          >  1:2351104/1‑50 (MQ=255)      gagaAAGGCGACGTGGTTCGAAGACGCCCACGACGATGCAGGAACTCCGc                                         <  1:2755887/50‑1 (MQ=255)        gaAAGGCGACGTGGTTCGAAGACGCCCACGACGATGCAGGAACTCCGCTg                                       >  1:549619/1‑50 (MQ=255)        gaAAGGCGACGTGGTTCGAAGACGCCCACGACGATGCAGGAACTCCGCTg                                       >  1:497961/1‑50 (MQ=255)          aaGGCGACGTGGTTCGAAGACGCCCACGACGATGCAGGAACTCCGCTGtt                                     >  1:2182592/1‑50 (MQ=255)           aGGCGACGTGGTTCGAAGACGCCCACGACGATGCAGGAACTCCGCTGttt                                    <  1:2285801/50‑1 (MQ=255)              cGACGTGGTTCGAAGACGCCCACGACGATGCAGGAACTCCGCTGTTtctc                                 <  1:2198319/50‑1 (MQ=255)               gACGTGGTTCGAAGACGCCCACGACGATGCAGGAACTCCGCTGTTTCTcc                                <  1:366670/50‑1 (MQ=255)                  gTGGTTCGAAGACGCCCACGACGATGCAGGAACTCCGCTGTTTCTCCggg                             >  1:2178557/1‑50 (MQ=255)                    ggTTCGAAGACGCCCACGACGATGCAGGAACTCCGCTGTTTCTCCGGGc                            >  1:1627695/1‑49 (MQ=255)                     gTTCGAAGACGCCCACGACGATGCAGGAACTCCGCTGTTTCTCCGGGCTa                          <  1:1489134/50‑1 (MQ=255)                      ttCGAAGACGCCCACGACGATGCAGGAACTCCGCTGTTTCTCCGGGCTAc                         >  1:1497770/1‑50 (MQ=255)                       tCGAAGACGCCCACGACGATGCAGGAACTCCGCTGTTTCTCCGGGCTAcc                        >  1:1229788/1‑50 (MQ=255)                         gAAGACGCCCACGACGATGCAGGAACTCCGCTGTTTCTCCGGGCTACCg                       <  1:866065/49‑1 (MQ=255)                          aaGACGCCCACGACGATGCAGGAACTCCGCTGTTTCTCCGGGCTACCGCt                     <  1:1732040/50‑1 (MQ=255)                           aGACGCCCACGACGATGCAGGAACTCCGCTGTTTCTCCGGGCTACCGCtt                    >  1:711362/1‑50 (MQ=255)                             aCGCCCACGACGATGCAGGAACTCCGCTGTTTCTCCGGGCTACCGCtttt                  <  1:471239/50‑1 (MQ=255)                               gCCCACGACGATGCAGGAACTCCGCTGTTTCTCCGGGCTACCGCTTTTTa                <  1:1115236/50‑1 (MQ=255)                               gCCCACGACGATGCAGGAACTCCGCTGTTTCTCCGGGCTACCGCTTTTTa                >  1:2634586/1‑50 (MQ=255)                               gCCCACGACGATGCAGGAACTCCGCTGTTTCTCCGGGCTACCGCTTTTTa                <  1:456832/50‑1 (MQ=255)                                  cACGACGATGCAGGAACTCCGCTGTTTCTCCGGGCTACCGCTTTTTacac             >  1:2432788/1‑50 (MQ=255)                                   acgacgATGCAGGAACTCCGCTGTTTCTCCGGGCTACCGCTTTTTacac             >  1:2000530/1‑49 (MQ=255)                                    cgacgaTGCAGGAACTCCGCTGTTTCTCCGGGCTACCGCTTTTTACACt            >  1:1701825/1‑49 (MQ=255)                                     gacgaTGCAGGAACTCCGCTGTTTCTCCGGGCTACCGCTTTTTACACTa           >  1:1019353/1‑49 (MQ=255)                                      acgaTGCAGGAACTCCGCTGTTTCTCCGGGCTACCGCTTTTTACACTaaa         >  1:2701986/1‑50 (MQ=255)                                       cgaTGCAGGAACTCCGCTGTTTCTCCGGGCTACCGCTTTTTACACTAAAc        <  1:1852008/50‑1 (MQ=255)                                         aTGCAGGAACTCCGCTGTTTCTCCGGGCTACCGCTTTTTACACTAAACTc      <  1:106516/50‑1 (MQ=255)                                          tGCAGGAACTCCGCTGTTTCTCCGGGCTACCGCTTTTTACACTAAACTcc     <  1:1455542/50‑1 (MQ=255)                                            cAGGAACTCCGCTGTTTCTCCGGGCTACCGCTTTTTACACTAAACTCCa    <  1:1576496/49‑1 (MQ=255)                                            cAGGAACTCCGCTGTTTCTCCGGGCTACCGCTTTTTACACTAAACTCCa    <  1:1907330/49‑1 (MQ=255)                                            cAGGAACTCCGCTGTTTCTCCGGGCTACCGCTTTTTACACTAAACTCCAt   >  1:1864398/1‑50 (MQ=255)                                             aGGAACTCCGCTGTTTCTCCGGGCTACCGCTTTTTACACTAAACTCCATg  <  1:461312/50‑1 (MQ=255)                                             aGGAACTCCGCTGTTTCTCCGGGCTACCGCTTTTTACACTAAACTCCATg  <  1:2122995/50‑1 (MQ=255)                                                |                                               AATCGGAGAAAGGCGACGTGGTTCGAAGACGCCCACGACGATGCAGGCACTCCGCTGTTTCTCCGGGCTACCGCTTTTTACACTAAACTCCATG  >  NC_000913/4485859‑4485952

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 30 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑