Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	988,499	G→A	P163S (CCA→TCA)	asnS ←	asparagine‑‑tRNA ligase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	988,499	0	G	A	100.0%	116.9 / NA	30	P163S (CCA→TCA)	asnS	asparagine‑‑tRNA ligase
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base A (15/15);  total (15/15)

CGGAACATTTCGCCTGCACCTTCGGTATCAGATGCGGTAATCAGTGGCGTTGAAACCCAGAAGAATCCCTGCTCGTTAAAGAAGCGATGCAG  >  NC_000913/988453‑988544                                               |                                              cgGAACATTTCGCCTGCACCTTCGGTATCAGATGCGGTAATCAGTGACGt                                            >  1:54542/1‑50 (MQ=255)  ggAACATTTCGCCTGCACCTTCGGTATCAGATGCGGTAATCAGTGACGtt                                           <  1:1067033/50‑1 (MQ=255)   gAACATTTCGCCTGCACCTTCGGTATCAGATGCGGTAATCAGTGACGTTg                                          <  1:2764448/50‑1 (MQ=255)   gAACATTTCGCCTGCACCTTCGGTATCAGATGCGGTAATCAGTGACGTTg                                          <  1:1014665/50‑1 (MQ=255)     aCATTTCGCCTGCACCTTCGGTATCAGATGCGGTAATCAGTGACGTTGaa                                        <  1:2029018/50‑1 (MQ=255)        tttCGCCTGCACCTTCGGTATCAGATGCGGTAATCAGTGACGTTGAAAcc                                     <  1:280537/50‑1 (MQ=255)        tttCGCCTGCACCTTCGGTATCAGATGCGGTAATCAGTGACGTTGAAAcc                                     <  1:1907196/50‑1 (MQ=255)          tCGCCTGCACCTTCGGTATCAGATGCGGTAATCAGTGACGTTGAAAcc                                     >  1:35867/1‑48 (MQ=255)            gCCTGCACCTTCGGTATCAGATGCGGTAATCAGTGACGTTGAAACCCaga                                 <  1:1402736/50‑1 (MQ=255)            gCCTGCACCTTCGGTATCAGATGCGGTAATCAGTGACGTTGAAACCCag                                  >  1:47985/1‑49 (MQ=255)            gCCTGCACCTTCGGTATCAGATGCGGTAATCAGTGACGTTGAAACCCag                                  >  1:490267/1‑49 (MQ=255)              cTGCACCTTCGGTATCAGATGCGGTAATCAGTGACGTTGAAACCCag                                  >  1:464157/1‑47 (MQ=255)                gCACCTTCGGTATCAGATGCGGTAATCAGTGACGTTGAAACCCAgaagaa                             >  1:2076715/1‑50 (MQ=255)                 cACCTTCGGTATCAGATGCGGTAATCAGTGACGTTGAAACCCAGAAGAAt                            <  1:1897259/50‑1 (MQ=255)                      tCGGTATCAGATGCGGTAATCAGTGACGTTGAAACCCAGAAGAATCCCTg                       >  1:410844/1‑50 (MQ=255)                       cGGTATCAGATGCGGTAATCAGTGACGTTGAAACCCAGAAGAATCCCt                        <  1:280606/48‑1 (MQ=255)                       cGGTATCAGATGCGGTAATCAGTGACGTTGAAACCCAGAAGAATCCCt                        <  1:148036/48‑1 (MQ=255)                          tATCAGATGCGGTAATCAGTGACGTTGAAACCCAGAAGAATCCCTGCTCg                   <  1:948909/50‑1 (MQ=255)                           aTCAGATGCGGTAATCAGTGACGTTGAAACCCAGAAGAATCCCTGCTCGt                  >  1:1764146/1‑50 (MQ=255)                             cAGATGCGGTAATCAGTGACGTTGAAACCCAGAAGAATCCCTGCTCGtt                 >  1:1084727/1‑49 (MQ=255)                              aGATGCGGTAATCAGTGACGTTGAAACCCAGAAGAATCCCTGCTCGTTa                <  1:2010619/49‑1 (MQ=255)                              aGATGCGGTAATCAGTGACGTTGAAACCCAGAAGAATCCCTGCTCGTTa                >  1:309760/1‑49 (MQ=255)                                aTGCGGTAATCAGTGACGTTGAAACCCAGAAGAATCCCTGCTCGTTa                >  1:1976321/1‑47 (MQ=255)                                   cGGTAATCAGTGACGTTGAAACCCAGAAGAATCCCTGCTCGTTAaagaa           <  1:2750210/49‑1 (MQ=255)                                    ggTAATCAGTGACGTTGAAACCCAGAAGAATCCCTGCTCGTTAAAGAAGc         >  1:743639/1‑50 (MQ=255)                                     gTAATCAGTGACGTTGAAACCCAGAAGAATCCCTGCTCGTTAAAGAAGCg        <  1:1025934/50‑1 (MQ=255)                                        aTCAGTGACGTTGAAACCCAGAAGAATCCCTGCTCGTTAAAGAAGCGAt      <  1:813636/49‑1 (MQ=255)                                        aTCAGTGACGTTGAAACCCAGAAGAATCCCTGCTCGTTAAAGAAGCGATg     >  1:2466925/1‑50 (MQ=255)                                        aTCAGTGACGTTGAAACCCAGAAGAATCCCTGCTCGTTAAAGAAGCGATg     >  1:2186751/1‑50 (MQ=255)                                           aGTGACGTTGAAACCCAGAAGAATCCCTGCTCGTTAAAGAAGCGATGCAg  >  1:48485/1‑50 (MQ=255)                                               |                                              CGGAACATTTCGCCTGCACCTTCGGTATCAGATGCGGTAATCAGTGGCGTTGAAACCCAGAAGAATCCCTGCTCGTTAAAGAAGCGATGCAG  >  NC_000913/988453‑988544

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑