Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	1,240,439	C→T	V60V (GTC→GTT)  D545N (GAC→AAC)	ymgM → cvrA ←	protein YmgM putative K(+):H(+) antiporter

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	1,240,439	0	C	T	100.0%	93.2 / NA	24	V60V (GTC→GTT)  D545N (GAC→AAC)	ymgM cvrA	protein YmgM putative K(+):H(+) antiporter
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base T (11/13);  total (11/13)

CTTTCTCGGCCACCGTCCAGATCATCCCGGCAAACTCTACCTGGTCACCGACAACCGGTGCCGCGCCTAACAACTGCTGGACAATTTCA  >  NC_000913/1240394‑1240482                                              |                                            ctttctCGGCCACCGTCCAGATCATCCCGGCAAACTCTACCTGGTTAcc                                          <  1:8463/49‑1 (MQ=255) ctttctCGGCCACCGTCCAGATCATCCCGGCAAACTCTACCTGGTTAcc                                          >  1:761370/1‑49 (MQ=255) ctttctCGGCCACCGTCCAGATCATCCCGGCAAACTCTACCTGGTTAcc                                          >  1:2323553/1‑49 (MQ=255)  tttctCGGCCACCGTCCAGATCATCCCGGCAAACTCTACCTGGTTACCGa                                        >  1:353861/1‑50 (MQ=255)   ttctCGGCCACCGTCCAGATCATCCCGGCAAACTCTACCTGGTTACCGa                                        >  1:1112438/1‑49 (MQ=255)    tctcGGCCACCGTCCAGATCATCCCGGCAAACTCTACCTGGTTACCg                                         <  1:1180147/47‑1 (MQ=255)     ctcGGCCACCGTCCAGATCATCCCGGCAAACTCTACCTGGTTACCGACaa                                     >  1:1380765/1‑50 (MQ=255)     ctcGGCCACCGTCCAGATCATCCCGGCAAACTCTACCTGGTTACCGACaa                                     >  1:922469/1‑50 (MQ=255)     ctcGGCCACCGTCCAGATCATCCCGGCAAACTCTACCTGGTTACCGACa                                      <  1:1373204/49‑1 (MQ=255)        ggCCACCGTCCAGATCATCCCGGCAAACTCTACCTGGTTACCGACAAcc                                   <  1:943998/49‑1 (MQ=255)              cGTCCAGATCATCCCGGCAAACTCTACCTGGTTACCGACAACCGGTGCcg                            >  1:2756496/1‑50 (MQ=255)               gTCCAGATCATCCCGGCAAACTCTACCTGGTTACCGACAACCGGTGCcgc                           <  1:1060032/50‑1 (MQ=255)                    gATCATCCCGGCAAACTCTACCTGGCTACCGACAACCGGTGCCGCGCCt                       >  1:2276002/1‑49 (MQ=255)                      tcatcCCGGCAAACTCTACCTGGTTACCGACAACCGGTGCCGCGCCTaac                    <  1:2467270/50‑1 (MQ=255)                        atcCCGGCAAACTCTACCTGGTTACCGACAACCGGTGCCGCGCCTaaca                   <  1:2178469/49‑1 (MQ=255)                           ccGGCAAACTCTACCTGGTTACCGACAACCGGTGCCGCGCCTAACAActg               >  1:1812822/1‑50 (MQ=255)                            cGGCAAACTCTACCTGGTTACCGACAACCGGTGCCGCGCCTAACAActgc              >  1:2003690/1‑50 (MQ=255)                             ggCAAACTCTACCTGGTTACCGACAACCGGTGCCGCGCCTAACAActgct             <  1:328561/50‑1 (MQ=255)                              gCAAACTCTACCTGGTTACCGACAACCGGTGCCGCGCCTAACAActgctg            <  1:2358290/50‑1 (MQ=255)                              gCAAACTCTACCTGGTTACCGACAACCGGTGCCGCGCCTAACAActgctg            <  1:2749452/50‑1 (MQ=255)                               cAAACTCTACCTGGTTACCGACAACCGGTGCCGCGCCTAACAActgctg            >  1:2605169/1‑49 (MQ=255)                               cAAACTCTACCTGGTTACCGACAACCGGTGCCGCGCCTAACAACTGCTgg           <  1:1883338/50‑1 (MQ=255)                                  aCTCTACCTGGTTACCGACAACCGGTGCCGCGCCTAACAACTGCTGGACa        <  1:233600/50‑1 (MQ=255)                                        ccTGGTTACCGACAACCGGTGCCGCGCCTAACAACTGCTGGACAATTTCa  <  1:45872/50‑1 (MQ=255)                                              |                                            CTTTCTCGGCCACCGTCCAGATCATCCCGGCAAACTCTACCTGGTCACCGACAACCGGTGCCGCGCCTAACAACTGCTGGACAATTTCA  >  NC_000913/1240394‑1240482

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 31 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑