Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	1,382,555	C→T	S203S (AGC→AGT)	ompG →	outer membrane porin G

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	1,382,555	0	C	T	100.0%	112.2 / NA	28	S203S (AGC→AGT)	ompG	outer membrane porin G
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base T (7/21);  total (7/21)

GCGAGTGAACTATTATCTCGAGCGCGGCTTCAATATGGACGACAGCCGCAATAACGGTGAGTTTTCCACGCAAGAAATTCGCGCCTATTTGC  >  NC_000913/1382510‑1382601                                              |                                               gCGAGTGAACTATTATCTCGAGCGCGGCTTCAATATGGACGACAGTCGc                                             <  1:1271407/49‑1 (MQ=255)  cGAGTGAACTATTATCTCGAGCGCGGCTTCAATATGGACGACAGTCGCaa                                           <  1:83019/50‑1 (MQ=255)   gAGTGAACTATTATCTCGAGCGCGGCTTCAATATGGACGACAGTCGCAAt                                          <  1:1003234/50‑1 (MQ=255)    aGTGAACTATTATCTCGAGCGCGGCTTCAATATGGACGACAGTCGCAAt                                          >  1:1767772/1‑49 (MQ=255)    aGTGAACTATTATCTCGAGCGCGGCTTCAATATGGACGACAGTCGCAATa                                         <  1:787215/50‑1 (MQ=255)     gTGAACTATTATCTCGAGCGCGGCTTCAATATGGACGACAGTCGCAATaa                                        <  1:2092153/50‑1 (MQ=255)      tGAACTATTATCTCGAGCGCGGCTTCAATATGGACGACAGTCGCAATAAc                                       <  1:2008941/50‑1 (MQ=255)           tattatCTCGAGCGCGGCTTCAATATGGACGACAGTCGCAATAACGGt                                    >  1:1240893/1‑48 (MQ=255)                tctcGAGCGCGGCTTCAATATGGACGACAGTCGCAATAACGGTGAGttt                              <  1:1237250/49‑1 (MQ=255)                tctcGAGCGCGGCTTCAATATGGACGACAGTCGCAATAACGGTGAGttt                              <  1:2094325/49‑1 (MQ=255)                 ctcGAGCGCGGCTTCAATATGGACGACAGTCGCAATAACGGTGAGTTTTc                            >  1:43389/1‑50 (MQ=255)                    gAGCGCGGCTTCAATATGGACGACAGTCGCAATAACGGTGAGTTTTc                            <  1:2199955/47‑1 (MQ=255)                          ggCTTCAATATGGACGACAGTCGCAATAACGGTGAGTTTTCCACGCAAGa                   <  1:2210890/50‑1 (MQ=255)                          ggCTTCAATATGGACGACAGTCGCAATAACGGTGAGTTTTCCACGCAAGa                   <  1:252713/50‑1 (MQ=255)                          ggCTTCAATATGGACGACAGTCGCAATAACGGTGAGTTTTCCACGCAAGa                   <  1:1717090/50‑1 (MQ=255)                             ttCAATATGGACGACAGTCGCAATAACGGTGAGTTTTCCACGCAAGAAAt                >  1:2252288/1‑50 (MQ=255)                               cAATATGGACGACAGTCGCAATAACGGTGAGTTTTCCACGCAAGAAAt                <  1:283851/48‑1 (MQ=255)                               cAATATGGACGACAGTCGCAATAACGGTGAGTTTTCCACGCAAGAAAt                <  1:1623724/48‑1 (MQ=255)                                aaTATGGACGACAGTCGCAATAACGGTGAGTTTTCCACGCAAGAAATTcg             >  1:2381645/1‑50 (MQ=255)                                aaTATGGACGACAGTCGCAATAACGGTGAGTTTTCCACGCAAGAAATTcg             <  1:2491804/50‑1 (MQ=255)                                aaTATGGACGACAGTCGCAATAACGGTGAGTTTTCCACGCAAGAAATTc              <  1:72816/49‑1 (MQ=255)                                    tGGACGACAGTCGCAATAACGGTGAGTTTTCCACGCAAGAAATTCGCGcc         >  1:925043/1‑50 (MQ=255)                                     ggACGACAGTCGCAATAACGGTGAGTTTTCCACGCAAGAAATTCGCGcc         >  1:1481337/1‑49 (MQ=255)                                       acgacAGTCGCAATAACGGTGAGTTTTCCACGCAAGAAATTCGCGCCTAt      <  1:1581008/50‑1 (MQ=255)                                        cgacAGTCGCAATAACGGTGAGTTTTCCACGCAAGAAATTCGCGCCTAtt     <  1:1982133/50‑1 (MQ=255)                                          acAGTCGCAATAACGGTGAGTTTTCCACGCAAGAAATTCGCGCCTATTTg   <  1:449/50‑1 (MQ=255)                                          acAGTCGCAATAACGGTGAGTTTTCCACGCAAGAAATTCGCGCCTATTTg   <  1:674026/50‑1 (MQ=255)                                           cAGTCGCAATAACGGTGAGTTTTCCACGCAAGAAATTCGCGCCTATTTGc  <  1:87147/50‑1 (MQ=255)                                              |                                               GCGAGTGAACTATTATCTCGAGCGCGGCTTCAATATGGACGACAGCCGCAATAACGGTGAGTTTTCCACGCAAGAAATTCGCGCCTATTTGC  >  NC_000913/1382510‑1382601

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑