Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	1,626,996	C→T	D129N (GAT→AAT)	dcp ←	peptidyl‑dipeptidase Dcp

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	1,626,996	0	C	T	100.0%	111.7 / NA	28	D129N (GAT→AAT)	dcp	peptidyl‑dipeptidase Dcp
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base T (16/12);  total (16/12)

ACAAAACGTTGATGAATCACCTCCACCAGGCGGATGGATTCACTATCAAGCCCCAGGGATTCACGGCGCTGCCAGACAGCATCTAC  >  NC_000913/1626950‑1627035                                               |                                        aCAAAACGTTGATGAATCACCTCCACCAGGCGGATGGATTCACTATTAAg                                      >  1:1298668/1‑50 (MQ=255) aCAAAACGTTGATGAATCACCTCCACCAGGCGGATGGATTCACTATTAAg                                      <  1:1449641/50‑1 (MQ=255)  cAAAACGTTGATGAATCACCTCCACCAGGCGGATGGATTCACTATTAAGc                                     >  1:632445/1‑50 (MQ=255)    aaaCGTTGATGAATCACCTCCACCAGGCGGATGGATTCACTATTAAGccc                                   <  1:340028/50‑1 (MQ=255)     aaCGTTGATGAATCACCTCCACCATGCGGATGGATTCACTATTAAGcccc                                  <  1:535196/50‑1 (MQ=255)     aaCGTTGATGAATCACCTCCACCAGGCGGATGGATTCACTATTAAGccc                                   >  1:212814/1‑49 (MQ=255)       cGTTGATGAATCACCTCCACCAGGCGGATGGATTCACTATTAAGCCCCAg                                >  1:665364/1‑50 (MQ=255)           gatgaATCACCTCCACCAGGCGGATGGATTCACTATTAAGCCCCAGGGa                             <  1:2030793/49‑1 (MQ=255)           gatgaATCACCTCCACCAGGCGGATGGATTCACTATTAAGCCCCAGGGa                             <  1:1061226/49‑1 (MQ=255)            atgaATCACCTCCACCAGGCGGATGGATTCACTATTAAGCCCCAGGGAtt                           >  1:2522438/1‑50 (MQ=255)             tgaATCACCTCCACCAGGCGGATGGATTCACTATTAAGCCCCAGGGATTc                          <  1:2110620/50‑1 (MQ=255)               aaTCACCTCCACCAGGCGGATGGATTCACTATTAAGCCCCAGGGATTCAc                        >  1:89483/1‑50 (MQ=255)               aaTCACCTCCACCAGGCGGATGGATTCACTATTAAGCCCCAGGGATTCAc                        >  1:1231045/1‑50 (MQ=255)                aTCACCTCCACCAGGCGGATGGATTCACTATTAAGCCCCAGGGATTCACg                       <  1:1160349/50‑1 (MQ=255)                  cACCTCCACCAGGCGGATGGATTCACTATTAAGCCCCAGGGATTCACGgc                     >  1:2756841/1‑50 (MQ=255)                        caccaGGCGGATGGATTCACTATTAAGCCCCAGGTATTCACGGCGCTGcc               <  1:1909437/50‑1 (MQ=255)                          ccaGGCGGATGGATTCACTATTAAGCCCCAGGGATTCACGGCGCTGc                >  1:2572116/1‑47 (MQ=255)                          ccaGGCGGATGGATTCACTATTAAGCCCCAGGGATTCACGGCGCTGCCAg             <  1:244728/50‑1 (MQ=255)                            aGGCGGATGGATTCACTATTAAGCCCCAGGGATTCACGGCGCTGCCAGa            >  1:1969653/1‑49 (MQ=255)                            aGGCGGATGGATTCACTATTAAGCCCCAGGGATTCACGGCGCTGCCAGa            >  1:1501360/1‑49 (MQ=255)                              gCGGATGGATTCACTATTAAGCCCCAGGGATTCACGGCGCTGCCAGACAg         <  1:1577336/50‑1 (MQ=255)                               cGGATGGATTCACTATTAAGCCCCAGGGATTCACGGCGCTGCCAGACAGc        <  1:934592/50‑1 (MQ=255)                                 gatggatTCACTATTAAGCCCCAGGGATTCACGGCGCTGCCAGACAGCAt      >  1:2800686/1‑50 (MQ=255)                                 gatggatTCACTATTAAGCCCCAGGGATTCACGGCGCTGCCAGACAGCAt      >  1:2678769/1‑50 (MQ=255)                                  atggatTCACTATTAAGCCCCAGGGATTCACGGCGCTGCCAGACAGCATc     >  1:184494/1‑50 (MQ=255)                                   tggatTCACTATTAAGCCCCAGGGATTCACGGCGCTGCCAGACAGCATc     >  1:2372359/1‑49 (MQ=255)                                   tggatTCACTATTAAGCCCCAGGGATTCACGGCGCTGCCAGACAGCATCt    <  1:145131/50‑1 (MQ=255)                                     gatTCACTATTAAGCCCCAGGGATTCACGGCGCTGCCAGACAGCATCTAc  >  1:666571/1‑50 (MQ=255)                                               |                                        ACAAAACGTTGATGAATCACCTCCACCAGGCGGATGGATTCACTATCAAGCCCCAGGGATTCACGGCGCTGCCAGACAGCATCTAC  >  NC_000913/1626950‑1627035

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 18 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑