Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	119,028	T→C	V99A (GTC→GCC)	ampD →	1,6‑anhydro‑N‑acetylmuramoyl‑L‑alanine amidase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	119,028	0	T	C	100.0%	97.0 / NA	27	V99A (GTC→GCC)	ampD	1,6‑anhydro‑N‑acetylmuramoyl‑L‑alanine amidase
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base C (12/15);  total (12/15)

AATAGTCCAGTATGTTCCTTTCGATAAACGTGCATGGCATGCGGGAGTCTCTCAGTATCAGGGGCGCGAACGCTGCAATGATTTTTCTATTG  >  NC_000913/118981‑119072                                                |                                             aaTAGTCCAGTATGTTCCTTTCGATAAACGTGCATGGCATGCGGGAGcct                                            <  1:1865465/50‑1 (MQ=255)    aGTCCAGTATGTTCCTTTCGATAAACGTGCATGGCATGCGGGAGcctct                                          >  1:158606/1‑49 (MQ=255)       ccAGTATGTTCCTTTCGATAAACGTGCATGGCATGCGGGAGCCTCTCAg                                       >  1:1993989/1‑49 (MQ=255)        cAGTATGTTCCTTTCGATAAACGTGCATGGCATGCGGGAGCCTCTCAGt                                      <  1:389075/49‑1 (MQ=255)        cAGTATGTTCCTTTCGATAAACGTGCATGGCATGCGGGAGCCTCTCAGTa                                     <  1:2165766/50‑1 (MQ=255)           tATGTTCCTTTCGATAAACGTGCATGGCATGCGGGAGCCTCTCAGTAt                                    >  1:1548841/1‑48 (MQ=255)             tGTTCCTTTCGATAAACGTGCATGGCATGCGGGAGCCTCTCAGTATCAgg                                >  1:2202703/1‑50 (MQ=255)             tGTTCCTTTCGATAAACGTGCATGGCATGCGGGAGCCTCTCAGTATCAg                                 <  1:652307/49‑1 (MQ=255)               ttCCTTTCGATAAACGTGCATGGCATGCGGGAGCCTCTCAGTATCAgggg                              <  1:2299693/50‑1 (MQ=255)                 ccTTTCGATAAACGTGCATGGCATGCGGGAGCCTCTCAGTATCAGGGgcg                            >  1:2439555/1‑50 (MQ=255)                 ccTTTCGATAAACGTGCATGGCATGCGGGAGCCTCTCAGTATCAGGGgc                             <  1:906848/49‑1 (MQ=255)                      cGATAAACGTGCATGGCATGCGGGAGCCTCTCAGTATCAGGGGCGCGaa                        <  1:1385789/49‑1 (MQ=255)                       gATAAACGTGCATGGCATGCGGGAGCCTCTCAGTATCAGGGGCGCGAAc                       <  1:1814165/49‑1 (MQ=255)                         tAAACGTGCATGGCATGCGGGAGCCTCTCAGTATCAGGGGCGCGAACGCt                    <  1:2661169/50‑1 (MQ=255)                           aaCGTGCATGGCATGCGGGAGCCTCTCAGTATCAGGGGCGCGAACGCTg                   >  1:2667925/1‑49 (MQ=255)                              gTGCATGGCATGCGGGAGCCTCTCAGTATCAGGGGCGCGAACGCTGCAAt               <  1:2186232/50‑1 (MQ=255)                              gTGCATGGCATGCGGGAGCCTCTCAGTATCAGGGGCGCGAACGCTGCAAt               >  1:409723/1‑50 (MQ=255)                              gTGCATGGCATGCGGGAGCCTCTCAGTATCAGGGGCGCGAACGCTGCAAt               <  1:1534192/50‑1 (MQ=255)                                  atggcatgCGGGAGCCTCTCAGTATCAGGGGCGCGAACGCTGCAATGAtt           <  1:2078955/50‑1 (MQ=255)                                   tggcatgCGGGAGCCTCTCAGTATCAGGGGCGCGAACGCTGCAATGAttt          <  1:1202519/50‑1 (MQ=255)                                   tggcatgCGGGAGCCTCTCAGTATCAGGGGCGCGAACGCTGCAATGAttt          >  1:1005447/1‑50 (MQ=255)                                     gcatgCGGGAGCCTCTCAGTATCAGGGGCGCGAACGCTGCAATGAtttt         <  1:2386845/49‑1 (MQ=255)                                      catgCGGGAGCCTCTCAGTATCAGGGGCGCGAACGCTGCAATGATTTTTc       >  1:1583735/1‑50 (MQ=255)                                       atgCGGGAGCCTCTCAGTATCAGGGGCGCGAACGCTGCAATGATTTTTCt      <  1:752954/50‑1 (MQ=255)                                       atgCGGGAGCCTCTCAGTATCAGGGGCGCGAACGCTGCAATGATTTTTCt      <  1:987773/50‑1 (MQ=255)                                          cGGGAGCCTCTCAGTATCAGGGGCGCGAACGCTGCAATGATTTTTCTAtt   >  1:1746927/1‑50 (MQ=255)                                           gggAGCCTCTCAGTATCAGGGGCGCGAACGCTGCAATGATTTTTCTATTg  >  1:319102/1‑50 (MQ=255)                                           gggAGCCTCTCAGTATCAGGGGCGCGAACGCTGCAATGATTTTTCTATTg  >  1:343628/1‑50 (MQ=255)                                           gggAGCCTCTCAGTATCAGGGGCGCGAACGCTGCAATGATTTTTCTATTg  >  1:228613/1‑50 (MQ=255)                                                |                                             AATAGTCCAGTATGTTCCTTTCGATAAACGTGCATGGCATGCGGGAGTCTCTCAGTATCAGGGGCGCGAACGCTGCAATGATTTTTCTATTG  >  NC_000913/118981‑119072

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑