Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	1,662,872	C→T	R773W (CGG→TGG)	ynfF →	putative selenate reductase YnfF

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	1,662,872	0	C	T	100.0%	69.4 / NA	18	R773W (CGG→TGG)	ynfF	putative selenate reductase YnfF
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base T (9/9);  total (9/9)

CCGCCATCGGGCAAGGTGCGTGGCTTAAAGCGGATATGTTTGGTGACCGGGTCGATCACGGCGGCAGTATCAATATTCTGACCTCTCACCGCC  >  NC_000913/1662825‑1662917                                                |                                              ccGCCATCGGGCAAGGTGCGTGGCTTAAAGCGGATATGTTTGGTGACTgg                                             <  1:1014619/50‑1 (MQ=255)  cGCCATCGGGCCAGGTGCGTGGCTTAAAGCGGATATGTTTGGTGTCTggg                                            >  1:2155348/1‑50 (MQ=38)   gCCATCGGGCAAGGTGCGTGGCTTGAAGCGGATATGTTTGGTGACTGGGt                                           >  1:512801/1‑50 (MQ=39)    ccATCGGGCAAGGTGCGTGGCTTAAAGCGGATATGTTTGGTGACTGGGTc                                          <  1:727482/50‑1 (MQ=255)         gggCAAGGTGCGTGGCTTAAAGCGGATATGTTTGGTGACTGGGTCGATCa                                     <  1:232029/50‑1 (MQ=255)          ggCAAGGTGCGTGGCTTAAAGCGGATATGTTTGGTGACTGGGTCGATCAc                                    <  1:709759/50‑1 (MQ=255)          ggCAAGGTGCGTGGCTTAAAGCGGATATGTTTGGTGACTGGGTCGATCAc                                    <  1:558664/50‑1 (MQ=255)                 tGCGTGGCTTAAAGCGGATATGTTTGGTGACTGGGTCGATCAcggcgg                               >  1:2526142/1‑48 (MQ=255)                       gCTTAAAGCGGATATGTTTGGTGACTGGGTCGATCACGGCGGCAGTATc                        <  1:1051858/49‑1 (MQ=255)                         ttAAAGCGGATATGTTTGGTGACTGGGTCGATCACGGCGGCAGTATCaa                      >  1:2588814/1‑49 (MQ=255)                         ttAAAGCGGATATGTTTGGTGACTGGGTCGATCACGGCGGCAGTATCaa                      >  1:494958/1‑49 (MQ=255)                           aaaGCGGATATGTTTGGTGACTGGGTCGATCACGGCGGCAGTATCAatat                   >  1:1231058/1‑50 (MQ=255)                              gCGGATATGTTTGGTGACTGGGTCGATCACGGCGGCAGTATCAATATTCt                <  1:400835/50‑1 (MQ=255)                               cGGATATGTTTGGTGACTGGGTCGATCACGGCGGCAGTATCAATATTCt                >  1:2260009/1‑49 (MQ=255)                                    atGTTTGGTGACTGGGTCGATCACGGCGGCAGTATCAATATTCTGACctc          >  1:390720/1‑50 (MQ=255)                                    atGTTTGGTGACTGGGTCGATCACGGCGGCAGTATCAATATTCTGACctc          >  1:1024566/1‑50 (MQ=255)                                       tttGGTGACTGGGTCGATCACGGCGGCAGTATCAATATTCTGACCtctc        <  1:1725582/49‑1 (MQ=255)                                            tGACTGGGTCGATCACGGCGGCAGTATCAATATTCTGACCTCTCACCGcc  <  1:571881/50‑1 (MQ=255)                                                |                                              CCGCCATCGGGCAAGGTGCGTGGCTTAAAGCGGATATGTTTGGTGACCGGGTCGATCACGGCGGCAGTATCAATATTCTGACCTCTCACCGCC  >  NC_000913/1662825‑1662917

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 17 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑