Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	1,697,216	T→C	intergenic (‑164/+57)	uidR ← / ← hdhA	DNA‑binding transcriptional repressor UidR/7‑alpha‑hydroxysteroid dehydrogenase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	1,697,216	0	T	C	100.0%	149.6 / NA	40	intergenic (‑164/+57)	uidR/hdhA	DNA‑binding transcriptional repressor UidR/7‑alpha‑hydroxysteroid dehydrogenase
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base C (22/18);  total (22/18)

GTTAACCAATAGCCGTGATTTGGCTGCCAGTTATTTAGCAGAGACGTTGATGAGTTTATCCCGGTAAACAACACGCACGGTTGTTTACCGGTC  >  NC_000913/1697170‑1697262                                               |                                               gTTAACCAATAGCCGTGATTTGGCTGCCAGTTATTTAGCAGAGACGCtga                                             >  1:1826870/1‑50 (MQ=255)  ttAACCAATAGCCGTGATTTGGCTGCCAGTTATTTAGCAGAGACGCtgat                                            <  1:113900/50‑1 (MQ=255)  ttAACCAATAGCCGTGATTTGGCTGCCAGTTATTTAGCAGAGACGCtgat                                            <  1:1417938/50‑1 (MQ=255)      ccAATAGCCGTGATTTGGCTGCCAGTTATTTAGCCGAGACGCTGATGAGt                                        >  1:2289271/1‑50 (MQ=255)      ccAATAGCCGTGATTTGGCTGCCAGTTATTTAGCAGAGACGCTGATGAGt                                        >  1:760441/1‑50 (MQ=255)      ccAATAGCCGTGATTTGGCTGCCAGTTATTTAGCAGAGACGCTGATGAGt                                        <  1:299610/50‑1 (MQ=255)        aaTAGCCGTGATTTGGCTGCCAGTTATTTAGCAGAGACGCTGATGAGttt                                      <  1:556616/50‑1 (MQ=255)        aaTAGCCGTGATTTGGCTGCCAGTTATTTAGCAGAGACGCTGATGAGttt                                      <  1:2801416/50‑1 (MQ=255)        aaTAGCCGTGATTTGGCTGCCAGTTATTTAGCAGAGACGCTGATGAGttt                                      <  1:1760205/50‑1 (MQ=255)        aaTAGCCGTGATTTGGCTGCCAGTTATTTAGCAGAGACGCTGATGAGtt                                       >  1:173718/1‑49 (MQ=255)         aTAGCCGTGATTTGGCTGCCAGTTATTTAGCAGAGACGCTGATGAGTTTa                                     >  1:502287/1‑50 (MQ=255)          tAGCCGTGATTTGGCTGCCAGTTATTTAGCAGAGACGCTGATGAGTTTAt                                    >  1:903397/1‑50 (MQ=255)             ccGTGATTTGGCTGCCAGTTATTTAGCAGAGACGCTGATGAGTTTATccc                                 <  1:2049971/50‑1 (MQ=255)              cGTGATTTGGCTGCCAGTTATTTAGCAGAGACGCTGATGAGTTTATCCCg                                >  1:56685/1‑50 (MQ=255)              cGTGATTTGGCTGCCAGTTATTTAGCAGAGACGCTGATGAGTTTATCCCg                                <  1:2601946/50‑1 (MQ=255)              cGTGATTTGGCTGCCAGTTATTTAGCAGAGACGCTGATGAGTTTATCCCg                                <  1:2008146/50‑1 (MQ=255)              cGTGATTTGGCTGCCAGTTATTTAGCAGAGACGCTGATGAGTTTATCCCg                                <  1:1161431/50‑1 (MQ=255)               gTGATTTGGCTGCCAGTTATTTAGCAGAGACGCTGATGAGTTTATCCCgg                               >  1:773105/1‑50 (MQ=255)               gTGATTTGGCTGCCAGTTATTTAGCAGAGACGCTGATGAGTTTATCCCgg                               >  1:2258189/1‑50 (MQ=255)                tGATTTGGCTGCCAGTTATTTAGCAGAGACGCTGATGAGTTTATCCCGGt                              <  1:1943468/50‑1 (MQ=255)                    ttGGCTGCCAGTTATTTAGCAGAGACGCTGATGAGTTTATCCCGGTaaa                           >  1:1515797/1‑49 (MQ=255)                    ttGGCTGCCAGTTATTTAGCAGAGACGCTGATGAGTTTATCCCGGTaaa                           >  1:463897/1‑49 (MQ=255)                     tGGCTGCCAGTTATTTAGCAGAGACGCTGATGAGTTTATCCCGGTAaac                          >  1:178149/1‑49 (MQ=255)                     tGGCTGCCAGTTATTTAGCAGAGACGCTGATGAGTTTATCCCGGTAaac                          >  1:1703384/1‑49 (MQ=255)                       gCTGCCAGTTATTTAGCAGAGACGCTGATGAGTTTATCCCGGTAaacaac                       <  1:734653/50‑1 (MQ=255)                           ccAGTTATTTAGCAGAGACGCTGATGAGTTTATCCCGGTAAACAacac                     <  1:1890069/48‑1 (MQ=255)                             aGTTATTTAGCAGAGACGCTGATGAGTTTATCCCGGTAAACAAcacgcac                 >  1:1675673/1‑50 (MQ=255)                             aGTTATTTAGCAGAGACGCTGATGAGTTTATCCCGGTAAACAAcacgcac                 >  1:2362136/1‑50 (MQ=255)                             aGTTATTTAGCAGAGACGCTGATGAGTTTATCCCGGTAAACAAcacgcac                 <  1:1657329/50‑1 (MQ=255)                             aGTTATTTAGCAGAGACGCTGATGAGTTTATCCCGGTAAACAAcacgcac                 <  1:419912/50‑1 (MQ=255)                              gTTATTTAGCAGAGACGCTGATGAGTTTATCCCGGTAAACAAcacgcacg                >  1:1812279/1‑50 (MQ=255)                                  tttAGCAGAGACGCTGATGAGTTTATCCCGGTAAACAACACGCACGgtt             <  1:607652/49‑1 (MQ=255)                                    tAGCAGAGACGCTGATGAGTTTATCCCGGTAAACAACACGCACGgttgtt          >  1:1659465/1‑50 (MQ=255)                                       cAGAGACGCTGATGAGTTTATCCCGGTAAACAACACGCACGGTTGTTTAc       >  1:1804772/1‑50 (MQ=255)                                       cAGAGACGCTGATGAGTTTATCCCGGTAAACAACACGCACGGTTGTTTAc       >  1:2143328/1‑50 (MQ=255)                                        agagACGCTGATGAGTTTATCCCGGTAAACAACACGCACGGTTGTTTAcc      <  1:303984/50‑1 (MQ=255)                                        agagACGCTGATGAGTTTATCCCGGTAAACAACACGCACGGTTGTTTAcc      <  1:2032793/50‑1 (MQ=255)                                        agagACGCTGATGAGTTTATCCCGGTAAACAACACGCACGGTTGTTTAcc      >  1:1028683/1‑50 (MQ=255)                                          agaCGCTGATGAGTTTATCCCGGTAAACAACACGCACGGTTGTTTACCgg    >  1:1616992/1‑50 (MQ=255)                                            aCGCTGATGAGTTTATCCCGGTAAACAACACGCACGGTTGTTTACCGGTc  >  1:671334/1‑50 (MQ=255)                                               |                                               GTTAACCAATAGCCGTGATTTGGCTGCCAGTTATTTAGCAGAGACGTTGATGAGTTTATCCCGGTAAACAACACGCACGGTTGTTTACCGGTC  >  NC_000913/1697170‑1697262

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑