Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	1,741,062	T→C	C384C (TGT→TGC)	ydhC →	putative transporter YdhC

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	1,741,062	0	T	C	100.0%	115.3 / NA	30	C384C (TGT→TGC)	ydhC	putative transporter YdhC
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base C (14/16);  total (14/16)

TCAACAGTAGTGCTGGTCGCGCTGGGTTACATGATGCAACGTTGTGAAGAAGTTGGCTGCCAGAATCATGGCAATGCCGAAGTCGCTCAT  >  NC_000913/1741018‑1741107                                             |                                              tCAACAGTAGTGCTGGTCGCGCTGGGTTACATGATGCAACGTTGCgaag                                           <  1:938189/49‑1 (MQ=255) tCAACAGTAGTGCTGGTCGCGCTGGGTTACATGATGCAACGTTGCgaag                                           >  1:248438/1‑49 (MQ=255)   aaCAGTAGTGCTGGTCGCGCTGGGTTACATGATGCAACGTTGCgaagaa                                         <  1:513320/49‑1 (MQ=255)   aaCAGTAGTGCTGGTCGCGCTGGGTTACATGATGCAACGTTGCGaagaag                                        >  1:1238274/1‑50 (MQ=255)   aaCAGTAGTGCTGGTCGCGCTGGGTTACATGATGCAACGTTGCGaagaag                                        >  1:595460/1‑50 (MQ=255)   aaCAGTAGTGCTGGTCGCGCTGGGTTACATGATGCAACGTTGCGaagaag                                        <  1:1170653/50‑1 (MQ=255)    aCAGTAGTGCTGGTCGCGCTGGGTTACATGATGCAACGTTGCGAAGAAGt                                       >  1:1494352/1‑50 (MQ=255)       gtagtGCTGGTCGCGCTGGGTTACATGATGCAACGTTGCGAAGAAGTTgg                                    <  1:1213793/50‑1 (MQ=255)         agtGCTGGTCGCGCTGGGTTACATGATGCAACGTTGCGAAGAAGTTGGCt                                  >  1:2195993/1‑50 (MQ=255)            gCTGGTCGCGCTGGGTTACATGATGCAACGTTGCGAAGAAGTTGGCTGc                                >  1:929227/1‑49 (MQ=255)             cTGGTCGCGCTGGGTTACATGATGCAACGTTGCGAAGAAGTTGGCTGc                                >  1:1698101/1‑48 (MQ=255)             cTGGTCGCGCTGGGTTACATGATGCAACGTTGCGAAGAAGTTGGCTGCCa                              <  1:1789185/50‑1 (MQ=255)                gTCGCGCTGGGTTACATGATGCAACGTTGCGAAGAAGTTGGCTGCCAg                             <  1:1271037/48‑1 (MQ=255)                   gcgcTGGGTTACATGATGCAACGTTGCGAAGAAGTTGGCTGCCAGAATc                         <  1:874173/49‑1 (MQ=255)                   gcgcTGGGTTACATGATGCAACGTTGCGAAGAAGTTGGCTGCCAGAATc                         <  1:528383/49‑1 (MQ=255)                    cgcTGGGTTACATGATGCAACGTTGCGAAGAAGTTGGCTGCCAGAATCAt                       <  1:1488898/50‑1 (MQ=255)                      cTGGGTTACATGATGCAACGTTGCGAAGAAGTTGGCTGCCAGAATCATgg                     >  1:766695/1‑50 (MQ=255)                         ggTTACATGATGCAACGTCGCGAAGAAGTTGGCTGCCAGAATCATGGc                    <  1:1532911/48‑1 (MQ=39)                          gTTACATGATGCAACGTTGCGAAGAAGTTGGCTGCCAGAATCATGGCAAt                 <  1:2706062/50‑1 (MQ=255)                          gTTACATGATGCAACGTTGCGAAGAAGTTGGCTGCCAGAATCATGGCAAt                 <  1:611358/50‑1 (MQ=255)                          gTTACATGATGCAACGTTGCGAAGAAGTTGGCTGCCAGAATCATGGCAAt                 <  1:195771/50‑1 (MQ=255)                            tACATGATGCAACGTTGCGAAGAAGTTGGCTGCCAGAATCATGGCAATGc               >  1:965580/1‑50 (MQ=255)                             aCATGATGCAACGTTGCGAAGAAGTTGGCTGCCAGAATCATGGCAATGcc              >  1:171795/1‑50 (MQ=255)                                    gCAACGTTGCGAAGAAGTTGGCTGCCAGAATCATGGCAATGCCGAAGTCg       >  1:607746/1‑50 (MQ=255)                                     cAACGTTGCGAAGAAGTTGGCTGCCAGAATCATGGCAATGCCGAAGTCGc      <  1:1587445/50‑1 (MQ=255)                                      aaCGTTGCGAAGAAGTTGGCTGCCAGAATCATGGCAATGCCGAAGTCGCt     <  1:1670040/50‑1 (MQ=255)                                         gTTGCGAAGAAGTTGGCTGCCAGAATCATGGCAATGCCGAAGTCGCTCa   >  1:1309104/1‑49 (MQ=255)                                         gTTGCGAAGAAGTTGGCTGCCAGAATCATGGCAATGCCGAAGTCGCTCa   >  1:2377279/1‑49 (MQ=255)                                         gTTGCGAAGAAGTTGGCTGCCAGAATCATGGCAATGCCGAAGTCGCTCa   >  1:1720008/1‑49 (MQ=255)                                         gTTGCGAAGAAGTTGGCTGCCAGAATCATGGCAATGCCGAAGTCGCTCAt  <  1:2541529/50‑1 (MQ=255)                                             |                                              TCAACAGTAGTGCTGGTCGCGCTGGGTTACATGATGCAACGTTGTGAAGAAGTTGGCTGCCAGAATCATGGCAATGCCGAAGTCGCTCAT  >  NC_000913/1741018‑1741107

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑