Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	1,776,533	G→A	G316D (GGC→GAC)	ydiF →	putative acetate‑CoA transferase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	1,776,533	0	G	A	100.0%	175.1 / NA	45	G316D (GGC→GAC)	ydiF	putative acetate‑CoA transferase
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base A (13/32);  total (13/32)

AAAGGCGCGGTGGGGAATGTCGGCGTCGGTATTGCTGACGGCATTGGCCTGGTCGCCCGAGAAGAAGGTTGTGCTGATGACTTTATTCTGAC  >  NC_000913/1776487‑1776578                                               |                                              aaaGGCGCGGTGGGGAATGTCGGCGTCGGTATTGCTGACGGCATTGACCt                                            >  1:1197589/1‑50 (MQ=255)  aaGGCGCGGTGGGGAATGTCGGCGTCGGTATTGCTGACGGCATTGACCt                                            <  1:962386/49‑1 (MQ=255)  aaGGCGCGGTGGGGAATGTCGGCGTCGGTATTGCTGACGGCATTGACCt                                            <  1:1957629/49‑1 (MQ=255)  aaGGCGCGGTGGGGAATGTCGGCGTCGGTATTGCTGACGGCATTGACCt                                            <  1:1866922/49‑1 (MQ=255)  aaGGCGCGGTGGGGAATGTCGGCGTCGGTATTGCTGACGGCATTGACCTg                                           >  1:1187309/1‑50 (MQ=255)  aaGGCGCGGTGGGGAATGTCGGCGTCGGTATTGCTGACGGCATTGACCTg                                           <  1:1688931/50‑1 (MQ=255)    ggCGCGGTGGGGAATGTCGGCGTCGGTATTGCTGACGGCATTGACCTGGt                                         <  1:542036/50‑1 (MQ=255)     gcgcGGTGGGGAATGTCGGCGTCGGTATTGCTGACGGCATTGACCTGGTc                                        <  1:2405205/50‑1 (MQ=255)       gcgGTGGGGAATGTCGGCGTCGGTATTGCTGACGGCATTGACCTGGTCGc                                      >  1:2740659/1‑50 (MQ=255)       gcgGTGGGGAATGTCGGCGTCGGTATTGCTGACGGCATTGACCTGGTCGc                                      >  1:258497/1‑50 (MQ=255)          gTGGGGAATGTCGGCGTCGGTATTGCTGACGGCATTGACCTGGTCGccc                                    >  1:1794070/1‑49 (MQ=255)          gTGGGGAATGTCGGCGTCGGTATTGCTGACGGCATTGACCTGGTCGCCCg                                   <  1:2672442/50‑1 (MQ=255)             gggAATGTCGGCGTCGGTATTGCTGACGGCATTGACCTGGTCGCCCgaga                                <  1:432098/50‑1 (MQ=255)              ggAATGTCGGCGTCGGTATTGCTGACGGCATTGACCTGGTCGCCCGagaa                               <  1:798775/50‑1 (MQ=255)                aaTGTCGGCGTCGGTATTGCTGACGGCATTGACCTGGTCGCCCGagaag                              >  1:521019/1‑49 (MQ=255)                  tGTCGGCGTCGGTATTGCTGACGGCATTGACCTGGTCGCCCGAGaagaag                           <  1:696177/50‑1 (MQ=255)                  tGTCGGCGTCGGTATTGCTGACGGCATTGACCTGGTCGCCCGAGaagaag                           <  1:1990622/50‑1 (MQ=255)                  tGTCGGCGTCGGTATTGCTGACGGCATTGACCTGGTCGCCCGAGaagaag                           >  1:490739/1‑50 (MQ=255)                    tCGGCGTCGGTATTGCTGACGGCATTGACCTGGTCGCCCGAGAAGAAGGt                         <  1:2359315/50‑1 (MQ=255)                      ggCGTCGGTATTGCTGACGGGATTGACCTGGTCGCCCGAGAAGAAGGTtg                       <  1:1081123/50‑1 (MQ=38)                      ggCGTCGGTATTGCTGACGGCATTGACCTGGTCGCCCGAGAAGAAGGtt                        <  1:2381308/49‑1 (MQ=255)                       gCGTCGGTATTGCTGACGGCATTGACCTGGTCGCCCGAGAAGAAGGTtgt                      <  1:2286344/50‑1 (MQ=255)                       gCGTCGGTATTGCTGACGGCATTGACCTGGTCGCCCGAGAAGAAGGTtgt                      <  1:2196967/50‑1 (MQ=255)                       gCGTCGGTATTGCTGACGGCATTGACCTGGTCGCCCGAGAAGAAGGTtgt                      <  1:219517/50‑1 (MQ=255)                        cGTCGGTATTGCTGACGGCATTGACCTGGTCGCCCGAGAAGAAGGTtgtg                     <  1:215079/50‑1 (MQ=255)                         gTCGGTATTGCTGACGGCATTGACCTGGTCGCCCGAGAAGAAGGTTGTGc                    <  1:1701452/50‑1 (MQ=255)                          tCGGTATTGCTGACGGCATTGACCTGGTCGCCCGAGAAGAAGGTTGTGCt                   <  1:2360541/50‑1 (MQ=255)                            ggTATTGCTGACGGCATTGACCTGGTCGCCCGAGAAGAAGGTTGTGCtga                 <  1:2588102/50‑1 (MQ=255)                            ggTATTGCTGACGGCATTGACCTGGTCGCCCGAGAAGAAGGTTGTGCtga                 >  1:766201/1‑50 (MQ=255)                            ggTATTGCTGACGGCATTGACCTGGTCGCCCGAGAAGAAGGTTGTGCtg                  <  1:31867/49‑1 (MQ=255)                             gTATTGCTGACGGCATTGACCTGGTCGCCCGAGAAGAAGGTTGTGCtgat                <  1:2465945/50‑1 (MQ=255)                              tATTGCTGACGGCATTGACCTGGTCGCCCGAGAAGAAGGTTGTGCtgatg               <  1:1259982/50‑1 (MQ=255)                                ttGCTGACGGCATTGACCTGGTCGCCCGAGAAGAAGGTTGTGCtgatga              <  1:1461544/49‑1 (MQ=255)                                ttGCTGACGGCATTGACCTGGTCGCCCGAGAAGAAGGTTGTGCTGATGAc             >  1:153270/1‑50 (MQ=255)                                   cTGACGGCATTGACCTGGTCGCCCGAGAAGAAGGTTGTGCTGATGACttt          <  1:862278/50‑1 (MQ=255)                                   cTGACGGCATTGACCTGGTCGCCCGAGAAGAAGGTTGTGCTGATGACttt          <  1:947046/50‑1 (MQ=255)                                     gACGGCATTGACCTGGTCGCCCGAGAAGAAGGTTGTGCTGATGACTTTAt        >  1:2759170/1‑50 (MQ=255)                                      aCGGCATTGACCTGGTCGCCCGAGAAGAAGGTTGTGCTGATGACTTTAt        <  1:549623/49‑1 (MQ=255)                                       cGGCATTGACCTGGTCGCCCGAGAAGAAGGTTGTGCTGATGACTTTATTc      <  1:2572302/50‑1 (MQ=255)                                        ggCATTGACCTGGTCGCCCGAGAAGAAGGTTGTGCTGATGACTTTATTCt     <  1:2071333/50‑1 (MQ=255)                                        ggCATTGACCTGGTCGCCCGAGAAGAAGGTTGTGCTGATGACTTTATTCt     <  1:675326/50‑1 (MQ=255)                                        ggCATTGACCTGGTCGCCCGAGAAGAAGGTTGTGCTGATGACTTTATTCt     >  1:2169991/1‑50 (MQ=255)                                         gCATTGACCTGGTCGCCCGAGAAGAAGGTTGTGCTGATGACTTTATTCTg    <  1:456602/50‑1 (MQ=255)                                          cATTGACCTGGTCGCCCGAGAAGAAGGTTGTGCTGATGACTTTATTCTGa   >  1:795619/1‑50 (MQ=255)                                           aTTGACCTGGTCGCCCGAGAAGAAGGTTGTGCTGATGACTTTATTCTGAc  >  1:1218870/1‑50 (MQ=255)                                               |                                              AAAGGCGCGGTGGGGAATGTCGGCGTCGGTATTGCTGACGGCATTGGCCTGGTCGCCCGAGAAGAAGGTTGTGCTGATGACTTTATTCTGAC  >  NC_000913/1776487‑1776578

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 29 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑