Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	1,811,208	T→C	V92A (GTT→GCT)	tcyP →	cystine/sulfocysteine:cation symporter

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	1,811,208	0	T	C	100.0%	127.3 / NA	34	V92A (GTT→GCT)	tcyP	cystine/sulfocysteine:cation symporter
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base C (18/16);  total (18/16)

CAAATGATCGTTATGCCGTTAGTCTTCGCCTCTATTCTGAGCGCGGTTGCCCGTCTGCATAACGCATCTCAGTTAGGCAAAATCAGTTTTCTG  >  NC_000913/1811162‑1811254                                               |                                               cAAATGATCGTTATGCCGTTAGTCTTCGCCTCTATTCTGAGCGCGGCTg                                              <  1:1455855/49‑1 (MQ=255)  aaaTGATCGTTATGCCGTTAGTCTTCGCCTCTATTCTGAGCGCGGCTGcc                                            >  1:949055/1‑50 (MQ=255)  aaaTGATCGTTATGCCGTTAGTCTTCGCCTCTATTCTGAGCGCGGCTGcc                                            >  1:187694/1‑50 (MQ=255)     tGATCGTTATGCCGTTAGTCTTCGCCTCTATTCTGAGCGCGGCTGCCCGt                                         >  1:969380/1‑50 (MQ=255)     tGATCGTTATGCCGTTAGTCTTCGCCTCTATTCTGAGCGCGGCTGCCCGt                                         >  1:772041/1‑50 (MQ=255)      gATCGTTATGCCGTTAGTCTTCGCCTCTATTCTGAGCGCGGCTGCCCGTc                                        >  1:407181/1‑50 (MQ=255)       aTCGTTATGCCGTTAGTCTTCGCCTCTATTCTGAGCGCGGCTGCCCGTCt                                       >  1:559741/1‑50 (MQ=255)       aTCGTTATGCCGTTAGTCTTCGCCTCTATTCTGAGCGCGGCTGCCCGTCt                                       >  1:2730469/1‑50 (MQ=255)        tCGTTATGCCGTTAGTCTTCGCCTCTATTCTGAGCGCGGCTGCCCGTCTg                                      <  1:1680536/50‑1 (MQ=255)          gTTATGCCGTTAGTCTTCGCCTCTATTCTGAGCGCGGCTGCCCGTCTGc                                     <  1:233648/49‑1 (MQ=255)                ccGTTAGTCTTCGCCTCTATTCTGAGCGCGGCTGCCCGTCTGCATAACg                               <  1:758265/49‑1 (MQ=255)                 cGTTAGTCTTCGCCTCTATTCTGAGCGCGGCTGCCCGTCTGCATAACGCa                             >  1:847169/1‑50 (MQ=255)                   ttAGTCTTCGCCTCTATTCTGAGCGCGGCTGCCCGTCTGCATAACGCAtc                           >  1:24304/1‑50 (MQ=255)                   ttAGTCTTCGCCTCTATTCTGAGCGCGGCTGCCCGTCTGCATAACGCAtc                           >  1:993020/1‑50 (MQ=255)                     aGTCTTCGCCTCTATTCTGAGCGCGGCTGCCCGTCTGCATAACGCAtct                          <  1:1101464/49‑1 (MQ=255)                     aGTCTTCGCCTCTATTCTGAGCGCGGCTGCCCGTCTGCATAACGCAtct                          >  1:2008750/1‑49 (MQ=255)                      gTCTTCGCCTCTATTCTGAGCGCGGCTGCCCGTCTGCATAACGCAtct                          >  1:1411692/1‑48 (MQ=255)                      gTCTTCGCCTCTATTCTGAGCGCGGCTGCCCGTCTGCATAACGCATCTCa                        <  1:1713543/50‑1 (MQ=255)                       tCTTCGCCTCTATTCTGAGCGCGGCTGCCCGTCTGCATAACGCATCTCAg                       <  1:1770492/50‑1 (MQ=255)                        cTTCGCCTCTATTCTGAGCGCGGCTGCCCGTCTGCATAACGCATCTCAGt                      >  1:2310851/1‑50 (MQ=255)                           cGCCTCTATTCTGAGCGCGGCTGCCCGTCTGCATAACGCATCTCAGtt                     <  1:1775052/48‑1 (MQ=255)                           cGCCTCTATTCTGAGCGCGGCTGCCCGTCTGCATAACGCATCTCAGt                      <  1:2234055/47‑1 (MQ=255)                            gCCTCTATTCTGAGCGCGGCTGCCCGTCTGCATAACGCATCTCAGTTAgg                  >  1:1155983/1‑50 (MQ=255)                            gCCTCTATTCTGAGCGCGGCTGCCCGTCTGCATAACGCATCTCAGTTAg                   <  1:1987163/49‑1 (MQ=255)                                 tATTCTGAGCGCGGCTGCCCGTCTGCATAACGCATCTCAGTTAGGCaaaa             >  1:2580590/1‑50 (MQ=255)                                 tATTCTGAGCGCGGCTGCCCGTCTGCATAACGCATCTCAGTTAGGCaa               <  1:2251250/48‑1 (MQ=255)                                      tGAGCGCGGCTGCCCGTCTGCATAACGCATCTCAGTTAGGCAAAATCAGt        >  1:577716/1‑50 (MQ=255)                                        aGCGCGGCTGCCCGTCTGCATAACGCATCTCAGTTAGGCAAAATCAGt        <  1:575872/48‑1 (MQ=255)                                         gcgcGGCTGCCCGTCTGCATAACGCATCTCAGTTAGGCAAAATCAGtttt     <  1:2686207/50‑1 (MQ=255)                                         gcgcGGCTGCCCGTCTGCATAACGCATCTCAGGTAGGCAAAATCAGtttt     <  1:225596/50‑1 (MQ=255)                                          cgcgGCTGCCCGTCTGCATAACGCATCTCAGTTAGGCAAAATCAGTTTTc    >  1:296132/1‑50 (MQ=255)                                          cgcgGCTGCCCGTCTGCATAACGCATCTCAGTTAGGCAAAATCAGTTTTc    >  1:2501100/1‑50 (MQ=255)                                            cgGCTGCCCGTCTGCATAACGCATCTCAGTTAGGCAAAATCAGTTTTCTg  <  1:587336/50‑1 (MQ=255)                                            cgGCTGCCCGTCTGCATAACGCATCTCAGTTAGGCAAAATCAGTTTTCTg  <  1:1577048/50‑1 (MQ=255)                                               |                                               CAAATGATCGTTATGCCGTTAGTCTTCGCCTCTATTCTGAGCGCGGTTGCCCGTCTGCATAACGCATCTCAGTTAGGCAAAATCAGTTTTCTG  >  NC_000913/1811162‑1811254

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑