Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	1,898,152	G→A	A407A (GCG→GCA)	sdaA →	L‑serine deaminase I

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	1,898,152	0	G	A	100.0%	122.2 / NA	30	A407A (GCG→GCA)	sdaA	L‑serine deaminase I
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base A (19/11);  total (19/11)

CATTGAGCGTAATGCCATTGCCTCTGTGAAGGCGATTAACGCCGCGCGGATGGCTCTGCGCCGCACCAGTGCACCGCGCGTCTCGCTGGA  >  NC_000913/1898107‑1898196                                              |                                             cATTGAGCGTAATGCCATTGCCTCTGTGAAGGCGATTAACGCCGCACGGa                                          >  1:1085624/1‑50 (MQ=255) cATTGAGCGTAATGCCATTGCCTCTGTGAAGGCGATTAACGCCGCACGGa                                          >  1:584198/1‑50 (MQ=255)  aTTGAGCGTAATGCCATTGCCTCTGTGAAGGCGATTAACGCCGCACGGAt                                         <  1:2509142/50‑1 (MQ=255)     gAGCGTAATGCCATTGCCTCTGTGAAGGCGATTAACGCCGCACGGATGGc                                      >  1:358492/1‑50 (MQ=255)      aGCGTAATGCCATTGCCTCTGTGAAGGCGATTAACGCCGCACGGATGGct                                     >  1:1746095/1‑50 (MQ=255)      aGCGTAATGCCATTGCCTCTGTGAAGGCGATTAACGCCGCACGGATGGct                                     >  1:121579/1‑50 (MQ=255)       gCGTAATGCCATTGCCTCTGTGAAGGCGATTAACGCCGCACGGATGGctc                                    <  1:205489/50‑1 (MQ=255)        cGTAATGCCATTGCCTCTGTGAAGGCGATTAACGCCGCACGGATGGctct                                   >  1:2292999/1‑50 (MQ=255)           aaTGCCATTGCCTCTGTGAAGGCGATTAACGCCGCACGGATGGCTCTgcg                                >  1:98073/1‑50 (MQ=255)            aTGCCATTGCCTCTGTGAAGGCGATTAACGCCGCACGGATGGCTCTgcgc                               >  1:1905327/1‑50 (MQ=255)            aTGCCATTGCCTCTGTGAAGGCGATTAACGCCGCACGGATGGCTCTgcgc                               >  1:2073428/1‑50 (MQ=255)              gCCATTGCCTCTGTGAAGGCGATTAACGCCGCACGGATGGCTCTGcgccg                             >  1:890115/1‑50 (MQ=255)                cATTGCCTCTGTGAAGGCGATTAACGCCGCACGGATGGCTCTGCGCCGCa                           >  1:1488786/1‑50 (MQ=255)                cATTGCCTCTGTGAAGGCGATTAACGCCGCACGGATGGCTCTGCGCCGCa                           >  1:2063754/1‑50 (MQ=255)                  ttGCCTCTGTGAAGGCGATTAACGCCGCACGGATGGCTCTGCGCCGCAcc                         <  1:762723/50‑1 (MQ=255)                  ttGCCTCTGTGAAGGCGATTAACGCCGCACGGATGGCTCTGCGCCGCAcc                         <  1:1398200/50‑1 (MQ=255)                    gCCTCTGTGAAGGCGATTAACGCCGCACGGATGGCTCTGCGCCGCACCAg                       >  1:2022728/1‑50 (MQ=255)                      ctctGTGAAGGCGATTAACGCCGCACGGATGGCTCTGCGCCGCACCAGTg                     >  1:86824/1‑50 (MQ=255)                         tgtgAAGGCGATTAACGCCGCACGGATGGCTCTGCGCCGCACCAGTGc                    <  1:113490/48‑1 (MQ=255)                         tgtgAAGGCGATTAACGCCGCACGGATGGCTCTGCGCCGCACCAGTGCAc                  >  1:975453/1‑50 (MQ=255)                           tgAAGGCGATTAACGCCGCACGGATGGCTCTGCGCCGCACCAGTGCAc                  <  1:550433/48‑1 (MQ=255)                                gCGATTAACGCCGCACGGATGGCTCTGCGCCGCACCAGTGCACCGCGCGt           >  1:2349423/1‑50 (MQ=255)                                gCGATTAACGCCGCACGGATGGCTCTGCGCCGCACCAGTGCACCGCGCGt           >  1:1729879/1‑50 (MQ=255)                                 cGATTAACGCCGCACGGATGGCTCTGCGCCGCACCAGTGCACCGCGCGtc          <  1:1706120/50‑1 (MQ=255)                                  gATTAACGCCGCACGGATGGCTCTGCGCCGCACCAGTGCACCGCGCGtct         >  1:2769864/1‑50 (MQ=255)                                   aTTAACGCCGCACGGATGGCTCTGCGCCGCACCAGTGCACCGCGCGtctc        <  1:429475/50‑1 (MQ=255)                                   aTTAACGCCGCACGGATGGCTCTGCGCCGCACCAGTGCACCGCGCGtctc        <  1:2399047/50‑1 (MQ=255)                                    ttAACGCCGCACGGATGGCTCTGCGCCGCACCAGTGCACCGCGCGTCTCg       <  1:2033683/50‑1 (MQ=255)                                        cgccgcACGGATGGCTCTGCGCCGCACCAGTGCACCGCGCGTCTCGCTgg   <  1:2796750/50‑1 (MQ=255)                                         gccgcACGGATGGCTCTGCGCCGCACCAGTGCACCGCGCGTCTCGCTGGa  >  1:581898/1‑50 (MQ=255)                                              |                                             CATTGAGCGTAATGCCATTGCCTCTGTGAAGGCGATTAACGCCGCGCGGATGGCTCTGCGCCGCACCAGTGCACCGCGCGTCTCGCTGGA  >  NC_000913/1898107‑1898196

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑