Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	1,941,784	A→G	V267A (GTC→GCC)	znuA ←	Zn(2(+)) ABC transporter periplasmic binding protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	1,941,784	0	A	G	100.0%	66.7 / NA	19	V267A (GTC→GCC)	znuA	Zn(2(+)) ABC transporter periplasmic binding protein
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base G (11/8);  total (11/8)

CAGGGGATCCAACGTTCCCATACGAACGGATGTCCCTCGTGCGACGCTCTCAACGACCGCTGGCCTGAACTGTGGCTCAGCAAAAAC  >  NC_000913/1941741‑1941827                                            |                                            cAGGGGATCCAACGTTCCCATACGAACGGATGTCCCTCGTGCGGCGctct                                       >  1:532304/1‑50 (MQ=255)   ggggATCCAACGTTCCCATACGAACGGATGTCCCTCGTGCGGCGCTCTCa                                     <  1:2188642/50‑1 (MQ=255)    gggATCCAACGTTCCCATACGAACGGATGTCCCTCGTGCGGCGCTCTCaa                                    >  1:489935/1‑50 (MQ=255)       aTCCAACGTTCCCATACGAACGGATGTCCCTCGTGCGGCGCTCTCAACGa                                 >  1:2157539/1‑50 (MQ=255)          cAACGTTCCCATACGAACGGATGTCCCTCGTGCGGCGCTCTCAACGACCg                              <  1:1257558/50‑1 (MQ=255)              gTTCCCATACGAACGGATGTCCCTCGTGCGGCGCTCTCAACGACCGCTgg                          >  1:660116/1‑50 (MQ=255)                   cATACGAACGGATGTCCCTCGTGCGGCGCTCTCAACGACCGCTGGCCTg                      >  1:691846/1‑49 (MQ=255)                   cATACGAACGGATGTCCCTCGTGCGGCGCTCTCAACGACCGCTGGCCTg                      >  1:1601867/1‑49 (MQ=255)                     tACGAACGGATGTCCCTCGTGCGGCTCTCTCAACGACCGCTGGCCTGAAc                   >  1:476243/1‑50 (MQ=255)                        gAACGGATGTCCCTCGTGCGGCGCTCTCAACGACCGCTGGCCTGAACtgt                >  1:2011727/1‑50 (MQ=255)                          aCGGATGTCCCTCGTGCGGCGCTCTCAACGACCGCTGGCCTGAACTGTgg              >  1:2579578/1‑50 (MQ=255)                            ggATGTCCCTCGTGCGGCGCTCTCAACGACCGCTGGCCTGAACTGTGGCt            >  1:1664772/1‑50 (MQ=255)                             gATGTCCCTCGTGCGGCGCTCTCAACGACCGCTGGCCTGAACTGTGGCt            >  1:1572111/1‑49 (MQ=255)                                 tCCCTCGTGCGGCGCTCTCAACGACCGCTGGCCTGAACTGTGGCTCAGCa       <  1:1375423/50‑1 (MQ=255)                                   ccTCGTGCGGCGCTCTCAACGACCGCTGGCCTGAACTGTGGCTCAGCaaa     <  1:153064/50‑1 (MQ=255)                                    cTCGTGCGGCGCTCTCAACGACCGCTGGCCTGAACTGTGGCTCAGCaaaa    <  1:218317/50‑1 (MQ=255)                                    cTCGTGCGGCGCTCTCAACGACCGCTGGCCTGAACTGTGGCTCAGCaaaa    <  1:1650659/50‑1 (MQ=255)                                    cTCGTGCGGCGCTCTCAACGACCGCTGGCCTGAACTGTGGCTCAGCaaaa    <  1:1620568/50‑1 (MQ=255)                                       gTGCGGCGCTCTCAACGACCGCTGGCCTGAACTGTGGCTCAGCAAAAAc  <  1:187815/49‑1 (MQ=255)                                            |                                            CAGGGGATCCAACGTTCCCATACGAACGGATGTCCCTCGTGCGACGCTCTCAACGACCGCTGGCCTGAACTGTGGCTCAGCAAAAAC  >  NC_000913/1941741‑1941827

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑