Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	214,885	C→T	T18I (ACC→ATC)	arfB →	peptidyl‑tRNA hydrolase, ribosome rescue factor

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	214,885	0	C	T	100.0%	103.6 / NA	27	T18I (ACC→ATC)	arfB	peptidyl‑tRNA hydrolase, ribosome rescue factor
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base T (15/12);  total (15/12)

TGTGATTTCCCGACATGTTGCTATTCCCGATGGTGAGCTTGAGATCACCGCCATTCGTGCGCAGGGCGCGGGCGGGCAGCATGTTAATAAGAC  >  NC_000913/214838‑214930                                                |                                              tgtgATTTCCCGACATGTTGCTATTCCCGATGGTGAGCTTGAGATCATCg                                             >  1:1868074/1‑50 (MQ=255) tgtgATTTCCCGACATGTTGCTATTCCCGATGGTGAGCTTGAGATCATCg                                             >  1:1965124/1‑50 (MQ=255)  gtgATTTCCCGACATGTTGCTATTCCCGATGGTGAGCTTGAGATCATCGc                                            >  1:2490811/1‑50 (MQ=255)    gATTTCCCGACATGTTGCTATTCCCGATGGTGAGCTTGAGATCATCGCCa                                          >  1:720918/1‑50 (MQ=255)      tttCCCGACATGTTGCTATTCCCGATGGTGAGCTTGAGATCATCGCCAtt                                        >  1:2608672/1‑50 (MQ=255)      tttCCCGACATGTTGCTATTCCCGATGGTGAGCTTGAGATCATCGCCAtt                                        >  1:764451/1‑50 (MQ=255)          ccGACATGTTGCTATTCCCGATGGTGAGCTTGAGATCATCGCCATTCGTg                                    <  1:2105383/50‑1 (MQ=255)              cATGTTGCTATTCCCGATGGTGAGCTTGAGATCATCGCCATTCGTgcg                                  >  1:1197613/1‑48 (MQ=255)               aTGTTGCTATTCCCGATGGTGAGCTTGAGATCATCGCCATTCGTGCGCAg                               <  1:2030179/50‑1 (MQ=255)               aTGTTGCTATTCCCGATGGTGAGCTTGAGATCATCGCCATTCGTGCGCAg                               >  1:672011/1‑50 (MQ=255)                  ttGCTATTCCCGATGGTGAGCTTGAGATCATCGCCATTCGTGCGCAGGgc                            <  1:2663960/50‑1 (MQ=255)                  ttGCTATTCCCGATGGTGAGCTTGAGATCATCGCCATTCGTGCGCAGGgc                            <  1:304249/50‑1 (MQ=255)                     cTATTCCCGATGGTGAGCTTGAGATCATCGCCATTCGTGCGCAGGGcgcg                         >  1:2146571/1‑50 (MQ=255)                      tATTCCCGATGGTGAGCTTGAGATCATCGCCATTCGTGCGCAGGGCgcgg                        >  1:2683007/1‑50 (MQ=255)                        ttCCCGATGGTGAGCTTGAGATCATCGCCATTCGTGCGCAGGGCgcgggc                      <  1:912115/50‑1 (MQ=255)                         tCCCGATGGTGAGCTTGAGATCATCGCCATTCGTGCGCAGGGCgcgggcg                     >  1:2069303/1‑50 (MQ=255)                            cGATGGTGAGCTTGAGATCATCGCCATTCGTGCGCAGGGCGcgggcggg                   >  1:2445627/1‑49 (MQ=255)                              aTGGTGAGCTTGAGATCATCGCCATTCGTGCGCAGGGCGCGGGCGGgcag                <  1:167468/50‑1 (MQ=255)                               tGGTGAGCTTGAGATCATCGCCATTCGTGCGCAGGGCGCGGGCGGgcagc               <  1:1613716/50‑1 (MQ=255)                               tGGTGAGCTTGAGATCATCGCCATTCGTGCGCAGGGCGCGGGCGGgcag                >  1:1241812/1‑49 (MQ=255)                               tGGTGAGCTTGAGATCATCGCCATTCGTGCGCAGGGCGCGGGCGGgcag                >  1:2260355/1‑49 (MQ=255)                                 gTGAGCTTGAGATCATCGCCATTCGTGCGCAGGGCGCGGGCGGgcagca              <  1:2239211/49‑1 (MQ=255)                                    aGCTTGAGATCATCGCCATTCGTGCGCAGGGCGCGGGCGGGCAGCATGt           <  1:219996/49‑1 (MQ=255)                                      cTTGAGATCATCGCCATTCGTGCGCAGGGCGCGGGCGGGCAGCATGTtaa        <  1:2192357/50‑1 (MQ=255)                                         gagaTCATCGCCATTCGTGCGCAGGGCGCGGGCGGGCAGCATGTtaata      <  1:98872/49‑1 (MQ=255)                                         gagaTCATCGCCATTCGTGCGCAGGGCGCGGGCGGGCAGCATGTtaat       <  1:319199/48‑1 (MQ=255)                                          agaTCATCGCCATTCGTGCGCAGGGCGCGGGCGGGCAGCATGTtaat       >  1:2196816/1‑47 (MQ=255)                                            atcatcGCCATTCGTGCGCAGGGCGCGGGCGGGCAGCATGTTAATAAGAc  <  1:2704307/50‑1 (MQ=255)                                                |                                              TGTGATTTCCCGACATGTTGCTATTCCCGATGGTGAGCTTGAGATCACCGCCATTCGTGCGCAGGGCGCGGGCGGGCAGCATGTTAATAAGAC  >  NC_000913/214838‑214930

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑