Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	2,662,004	T→C	E43G (GAA→GGA)	iscR ←	DNA‑binding transcriptional dual regulator IscR

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	2,662,004	0	T	C	100.0%	97.3 / NA	25	E43G (GAA→GGA)	iscR	DNA‑binding transcriptional dual regulator IscR
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base C (12/13);  total (12/13)

TACGCTGGAAACCAGACCATTTTTACGCAGACGGGAAAACAGTTGTTCCAGATAAGAAAGGGAAATTCCCTGACGTTCGGAAATATCAGCCA  >  NC_000913/2661958‑2662049                                               |                                              tACGCTGGAAACCAGACCATTTTTACGCAGACGGGAAAACAGTTGTCCCa                                            <  1:1236264/50‑1 (MQ=255)     cTGGAAACCAGACCATTTTTACGCAGACGGGAAAACAGTTGTCCCAGAt                                         >  1:1204707/1‑49 (MQ=255)     cTGGAAACCAGACCATTTTTACGCAGACGGGAAAACAGTTGTCCCAGAt                                         >  1:790817/1‑49 (MQ=255)     cTGGAAACCAGACCATTTTTACGCAGACGGGAAAACAGTTGTCCCAGAt                                         >  1:2230738/1‑49 (MQ=255)        gAAACCAGACCATTTTTACGCAGACGGGAAAACAGTTGTCCCAGATAAGa                                     <  1:18302/50‑1 (MQ=255)         aaaCCAGACCATTTTTACGCAGACGGGAAAACAGTTGTCCCAGATAAGa                                     >  1:938894/1‑49 (MQ=255)          aaCCAGACCATTTTTACGCAGACGGGAAAACAGTTGTCCCAGATAAGaaa                                   <  1:2210820/50‑1 (MQ=255)            ccAGACCATTTTTACGCAGACGGGAAAACAGTTGTCCCAGATAAGAAAgg                                 >  1:2699424/1‑50 (MQ=255)             cAGACCATTTTTACGCAGACGGGAAAACAGTTGTCCCAGATAAGAAAggg                                <  1:1403461/50‑1 (MQ=255)             cAGACCATTTTTACGCAGACGGGAAAACAGTTGTCCCAGATAAGAAAgg                                 >  1:44267/1‑49 (MQ=255)             cAGACCATTTTTACGCAGACGGGAAAACAGTTGTCCCAGATAAGAAAgg                                 >  1:2771810/1‑49 (MQ=255)                     ttttACGCAGACGGGAAAACAGTTGTCCCAGATAAGAAAGGGAAATTccc                        >  1:1311825/1‑50 (MQ=255)                      tttACGCAGACGGGAAAACAGTTGTCCCAGATAAGAAAGGGAAATTCCCt                       >  1:2217295/1‑50 (MQ=255)                        tACGCAGACGGGAAAACAGTTGTCCCAGATAAGAAAGGGAAATTCCCTg                      <  1:1304496/49‑1 (MQ=255)                          cGCAGACGGGAAAACAGTTGTCCCAGATAAGAAAGGGAAATTCCCTGACg                   <  1:2137756/50‑1 (MQ=255)                            cAGACGGGAAAACAGTTGTCCCAGATAAGAAAGGGAAATTCCCTGACGtt                 <  1:1611266/50‑1 (MQ=255)                             aGACGGGAAAACAGTTGTCCCAGATAAGAAAGGGAAATTCCCTGACGTTc                <  1:1903128/50‑1 (MQ=255)                              gACGGGAAAACAGTTGTCCCAGATAAGAAAGGGAAATTCCCTGACGTTCg               <  1:26487/50‑1 (MQ=255)                               aCGGGAAAACAGTTGTCCCAGATAAGAAAGGGAAATTCCCTGACGTTCgg              <  1:1925815/50‑1 (MQ=255)                                 gggAAAACAGTTGTCCCAGATAAGAAAGGGAAATTCCCTGACGTTCGGa             >  1:1429135/1‑49 (MQ=255)                                   gAAAACAGTTGTCCCAGATAAGAAAGGGAAATTCCCTGACGTTCGGaaa           >  1:2147453/1‑49 (MQ=255)                                   gAAAACAGTTGTCCCAGATAAGAAAGGGAAATTCCCTGACGTTCGGAAat          <  1:2708143/50‑1 (MQ=255)                                    aaaaCAGTTGTCCCAGATAAGAAAGGGAAATTCCCTGACGGTCGGAAata         >  1:2808820/1‑50 (MQ=255)                                      aaCAGTTGTCCCAGATAAGAAAGGGAAATTCCCTGACGTTCGGAAATATc       <  1:453031/50‑1 (MQ=255)                                        cAGTTGTCCCAGATAAGAAAGGGAAATTCCCTGACGTTCGGAAATATCAg     <  1:2277458/50‑1 (MQ=255)                                           ttgtcCCAGATAAGAAAGGGAAATTCCCTGACGTTCGGAAATATCAGCCa  <  1:1721600/50‑1 (MQ=255)                                               |                                              TACGCTGGAAACCAGACCATTTTTACGCAGACGGGAAAACAGTTGTTCCAGATAAGAAAGGGAAATTCCCTGACGTTCGGAAATATCAGCCA  >  NC_000913/2661958‑2662049

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 30 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑