| Predicted mutation | ||||||
|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | annotation | gene | description |
| RA | NC_000913 | 2,813,364 | T→C | V250A (GTC→GCC) | emrB → | multidrug efflux pump membrane subunit EmrB |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NC_000913 | 2,813,364 | 0 | T | C | 96.8% | 108.8 / ‑3.6 | 31 | V250A (GTC→GCC) | emrB | multidrug efflux pump membrane subunit EmrB |
| Reads supporting (aligned to +/- strand): ref base T (1/0); new base C (18/12); total (19/12) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.26e-01 | |||||||||||
TATCATCCTTACCGTGGTGGCGGTGGTGGCTATCTGCTTCCTGATTGTCTGGGAGCTGACCGACGATAACCCGATAGTCGATCTGTCGTTGTTT > NC_000913/2813317‑2813410 | tATCATCCTTACCGTGGTGGCGGTGGTGGCTATCTGCTTCCTGATTGCCt > 1:402794/1‑50 (MQ=255)tATCATCCTTACCGTGGTGGCGGTGGTGGCTATCTGCTTCCTGATTGCCt > 1:1675168/1‑50 (MQ=255) tcCTTACCGTGGTGGCGGTGGTGGCTATCTGCTTCCTGATTGCCTGGGAg < 1:48706/50‑1 (MQ=255) ccTTACCGTGGTGGCGGTGGTGGCTATCTGCTTCCTGATTGCCTGGGAGc > 1:1860101/1‑50 (MQ=255) ccTTACCGTGGTGGCGGTGGTGGCTATCTGCTTCCTGATTGCCTGGGAGc > 1:2796390/1‑50 (MQ=255) tACCGTGGTGGCGGTGGTGGCTATCTGCTTCCTGATTGTCTGGGAGCTGa > 1:1514612/1‑50 (MQ=255) aCCGTGGTGGCGGTGGTGGCTATCTGCTTCCTGATTGCCTGGGAGCTGa < 1:556984/49‑1 (MQ=255) aCCGTGGTGGCGGTGGTGGCTATCTGCTTCCTGATTGCCTGGGAGCTGAc > 1:1244599/1‑50 (MQ=255) ccGTGGTGGCGGTGGTGGCTATCTGCTTCCTGATTGCCTGGGAGCTGAcc > 1:751964/1‑50 (MQ=255) tggCGGTGGTGGCTATCTGCTTCCTGATTGCCTGGGAGCTGACcgacga > 1:313152/1‑49 (MQ=255) tggCGGTGGTGGCTATCTGCTTCCTGATTGCCTGGGAGCTGACcgacga > 1:1936915/1‑49 (MQ=255) gCGGTGGTGGCTATCTGCTTCCTGATTGCCTGGGAGCTGACCGACGATaa > 1:2552956/1‑50 (MQ=255) ggtggtGGCTATCTGCTTCCTGATTGCCTGGGAGCTGACCGACGATAAcc > 1:1137514/1‑50 (MQ=255) ggtggtGGCTATCTGCTTCCTGATTGCCTGGGAGCTGACCGACGATAAcc > 1:2492844/1‑50 (MQ=255) ggtggCTATCTGCTTCCTGATTGCCTGGGAGCTGACCGACGATAACCCGa > 1:868109/1‑50 (MQ=255) gtggCTATCTGCTTCCTGATTGCCTGGGAGCTGACCGACGATAACCCGAt < 1:407010/50‑1 (MQ=255) tggCTATCTGCTTCCTGATTGCCTGGGAGCTGACCGACGATAACCCGATa < 1:472651/50‑1 (MQ=255) gCTATCTGCTTCCTGATTGCCTGGGAGCTGACCGACGATAACCCGATAGt < 1:2618773/50‑1 (MQ=255) gCTATCTGCCTCCTGATTGCCTGGGAGCTGACCGACGATAACCCGATAGt < 1:46639/50‑1 (MQ=39) cTATCTGCTTCCTGATTGCCTGGGAGCTGACCGACGATAACCCGATAGTc > 1:902517/1‑50 (MQ=255) tCTGCTTCCTGATTGCCTGGGAGCTGACCGACGATAACCCGATAGTCg < 1:658653/48‑1 (MQ=255) tCTGCTTCCTGATTGCCTGGGAGCTGACCGACGATAACCCGATAGTCGAt > 1:1969063/1‑50 (MQ=255) tGCTTCCTGATTGCCTGGGAGCTGACCGACGATAACCCGATAGTCGATCt > 1:452042/1‑50 (MQ=255) gCTTCCTGATTGCCTGGGAGCTGACCGACGATAACCCGATAGTCGATCTg > 1:1244974/1‑50 (MQ=255) cTTCCTGATTGCCTGGGAGCTGACCGACGATAACCCGATAGTCGATc < 1:692063/47‑1 (MQ=255) ccTGATTGCCTGGGAGCTGACCGACGATAACCCGATAGTCGATCTGTc < 1:173783/48‑1 (MQ=255) ccTGATTGCCTGGGAGCTGACCGACGATAACCCGATAGTCGATCTGTc < 1:1227041/48‑1 (MQ=255) cTGATTGCCTGGGAGCTGACCGACGATAACCCGATAGTCGATCTGTCgtt > 1:2499873/1‑50 (MQ=255) tGATTGCCTGGGAGCTGACCGACGATAACCCGATAGTCGTTCTGTCgttg > 1:2643880/1‑50 (MQ=39) aTTGCCTGGGAGCTGACCGACGATAACCCGATAGTCGATCTGTCgttg < 1:648701/48‑1 (MQ=255) ttGCCTGGGAGCTGACCGACGATAACCCGATAGTCGATCTGTCGTTGttt < 1:445169/50‑1 (MQ=255) | TATCATCCTTACCGTGGTGGCGGTGGTGGCTATCTGCTTCCTGATTGTCTGGGAGCTGACCGACGATAACCCGATAGTCGATCTGTCGTTGTTT > NC_000913/2813317‑2813410 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 29 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |