Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	3,090,282	C→T	intergenic (+30/‑65)	yggI → / → endA	conserved protein YggI/DNA‑specific endonuclease I

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	3,090,282	0	C	T	100.0%	121.9 / NA	31	intergenic (+30/‑65)	yggI/endA	conserved protein YggI/DNA‑specific endonuclease I
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base T (15/16);  total (15/16)

GTTGCGAAATAACCATCTGAACTATCAGGAACTTTCCTGATCTGGCTGATTGCATACCAAAACAGCTTTCGCTACGTTGCTGGCTCGT  >  NC_000913/3090241‑3090328                                          |                                               gTTGCGAAATAACCATCTGAACTATCAGGAACTTTCCTGATTTGGCTGAt                                        >  1:420908/1‑50 (MQ=255)  ttGCGAAATAACCATCTGAACTATCAGGAACTTTCCTGATTTGGCTGAtt                                       <  1:1448204/50‑1 (MQ=255)    gCGAAATAACCATCTGAACTATCAGGAACTTTCCTGATTTGGCTGATTg                                      <  1:251109/49‑1 (MQ=255)     cGAAATAACCATCTGAACTATCAGGAACTTTCCTGATTTGGCTGATTGCa                                    <  1:1364798/50‑1 (MQ=255)       aaaTAACCATCTGAACTATCAGGAACTTTCCTGATTTGGCTGATTGCATa                                  <  1:831699/50‑1 (MQ=255)       aaaTAACCATCTGAACTATCAGGAACTTTCCTGATTTGGCTGATTGCATa                                  >  1:2207565/1‑50 (MQ=255)        aaTAACCATCTGAACTATCAGGAACTTTCCTGATTTGGCTGATTGCATAc                                 >  1:2669175/1‑50 (MQ=255)        aaTAACCATCTGAACTATCAGGAACTTTCCTGATTTGGCTGATTGCATAc                                 >  1:249591/1‑50 (MQ=255)         aTAACCATCTGAACTATCAGGAACTTTCCTGATTTGGCTGATTGCATAcc                                <  1:338884/50‑1 (MQ=255)         aTAACCATCTGAACTATCAGGAACTTTCCTGATTTGGCTGATTGCATAcc                                <  1:2669712/50‑1 (MQ=255)         aTAACCATCTGAACTATCAGGAACTTTCCTGATTTGGCTGATTGCATAc                                 >  1:760004/1‑49 (MQ=255)          tAACCATCTGAACTATCAGGAACTTTCCTGATTTGGCTGATTGCATAcc                                >  1:860689/1‑49 (MQ=255)             ccATCTGAACTATCAGGAACTTTCCTGATTTGGCTGATTGCATACCaaaa                            <  1:539517/50‑1 (MQ=255)                    aaCTATCAGGAACTTTCCTGATTTGGCTGATTGCATACCAAAACAGCttt                     <  1:2514448/50‑1 (MQ=255)                     aCTATCAGGAACTTTCCTGATTTGGCTGATTGCATACCAAAACAGCTTTc                    <  1:221283/50‑1 (MQ=255)                        aTCAGGAACTTTCCTGATTTGGCTGATTGCATACCAAAACAGCTTTCGc                  >  1:2259484/1‑49 (MQ=255)                         tCAGGAACTTTCCTGATTTGGCTGATTGCATACCAAAACAGCTTTCGc                  <  1:2356749/48‑1 (MQ=255)                         tCAGGAACTTTCCTGATTTGGCTGATTGCATACCAAAACAGCTTTCGCTa                <  1:1012228/50‑1 (MQ=255)                         tCAGGAACTTTCCTGATTTGGCTGATTGCATACCAAAACAGCTTTCGCTa                <  1:375481/50‑1 (MQ=255)                          cAGGAACTTTCCTGATTTGGCTGATTGCATACCAAAACAGCTTTCGCTAc               >  1:2288570/1‑50 (MQ=255)                          cAGGAACTTTCCTGATTTGGCTGATTGCATACCAAAACAGCTTTCGCTAc               >  1:682747/1‑50 (MQ=255)                           aGGAACTTTCCTGATTTGGCTGATTGCATACCAAAACAGCTTTCGCTAc               <  1:2213380/49‑1 (MQ=255)                           aGGAACTTTCCTGATTTGGCTGATTGCATACCAAAACAGCTTTCGCTACg              >  1:2303862/1‑50 (MQ=255)                             gAACTTTCCTGATTTGGCTGATTGCATACCAAAACAGCTTTCGCTACGtt            <  1:2767149/50‑1 (MQ=255)                             gAACTTTCCTGATTTGGCTGATTGCATACCAAAACAGCTTTCGCTACGtt            <  1:71270/50‑1 (MQ=255)                             gAACTTTCCTGATTTGGCTGATTGCATACCAAAACAGCTTTCGCTACGtt            <  1:1887365/50‑1 (MQ=255)                                  ttCCTGATTTGGCTGATTGCATACCAAAACAGCTTTCGCTACGTTGCTgg       >  1:1853241/1‑50 (MQ=255)                                    ccTGATTTGGCTGATTGCATACCAAAACAGCTTTCGCTACGTTGCTGGCt     >  1:322150/1‑50 (MQ=255)                                    ccTGATTTGGCTGATTGCATACCAAAACAGCTTTCGCTACGTTGCTGGCt     >  1:839845/1‑50 (MQ=255)                                       gATTTGGCTGATTGCATACCAAAACAGCTTTCGCTACGTTGCTGGCTCGt  >  1:926241/1‑50 (MQ=255)                                       gATTTGGCTGATTGCATACCAAAACAGCTTTCGCTACGTTGCTGGCTCGt  >  1:266782/1‑50 (MQ=255)                                          |                                               GTTGCGAAATAACCATCTGAACTATCAGGAACTTTCCTGATCTGGCTGATTGCATACCAAAACAGCTTTCGCTACGTTGCTGGCTCGT  >  NC_000913/3090241‑3090328

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑