Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	3,513,003	A→G	D212D (GAT→GAC)	trpS ←	tryptophan‑‑tRNA ligase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	3,513,003	0	A	G	100.0%	117.5 / NA	31	D212D (GAT→GAC)	trpS	tryptophan‑‑tRNA ligase
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base G (17/14);  total (17/14)

CGGAGTCAGTGACCGCACGTTTGATTTTCTTCACTACCGATTTCGGATCTTCCAGCAGGCCGATAACGTTATTGCGATTATCGTCAGACTTG  >  NC_000913/3512957‑3513048                                               |                                              cgGAGTCAGTGACCGCACGTTTGATTTTCTTCACTACCGATTTCGGGTCt                                            >  1:1160225/1‑50 (MQ=255)  ggAGTCAGTGACCGCACGTTTGATTTTCTTCACTACCGATTTCGGGTCtt                                           <  1:978756/50‑1 (MQ=255)    aGTCAGTGACCGCACGTTTGATTTTCTTCACTACCGATTTCGGGTCTTc                                          >  1:2219345/1‑49 (MQ=255)     gTCAGTGACCGCACGTTTGATTTTCTTCACTACCGATTTCGGGTCTTCca                                        <  1:1331374/50‑1 (MQ=255)      tCAGTGACCGCACGTTTGATTTTCTTCACTACCGATTTCGGGTCTTCcag                                       >  1:2324940/1‑50 (MQ=255)      tCAGTGACCGCACGTTTGATTTTCTTCACTACCGATTTCGGGTCTTCcag                                       <  1:777474/50‑1 (MQ=255)      tCAGTGACCGCACGTTTGATTTTCTTCACTACCGATTTCGGGTCTTCcag                                       <  1:501712/50‑1 (MQ=255)          tGACCGCACGTTTGATTTTCTTCACTACCGATTTCGGGTCTTCCAGCAgg                                   <  1:766294/50‑1 (MQ=255)          tGACCGCACGTTTGATTTTCTTCACTACCGATTTCGGGTCTTCCAGCAgg                                   <  1:1836279/50‑1 (MQ=255)          tGACCGCACGTTTGATTTTCTTCACTACCGATTTCGGGTCTTCCAGCAgg                                   >  1:2197341/1‑50 (MQ=255)            aCCGCACGTTTGATTTTCTTCACTACCGATTTCGGGTCTTCCAGCAGGcc                                 >  1:1986863/1‑50 (MQ=255)            aCCGCACGTTTGATTTTCTTCACTACCGATTTCGGGTCTTCCAGCAGGcc                                 >  1:1329224/1‑50 (MQ=255)             ccGCACGTTTGATTTTCTTCACTACCGATTTCGGGTCTTCCAGCAGGcc                                 >  1:2159037/1‑49 (MQ=255)              cGCACGTTTGATTTTCTTCACTACCGATTTCGGGTCTTCCAGCAGGCCGa                               <  1:1789427/50‑1 (MQ=255)                    tttGATTTTCTTCACTACCGATTTCGGGTCTTCCAGCAGGCCGATAACGt                         >  1:814435/1‑50 (MQ=255)                    tttGATTTTCTTCACTACCGATTTCGGGTCTTCCAGCAGGCCGATAACGt                         >  1:521852/1‑50 (MQ=255)                     ttGATTTTCTTCACTACCGATTTCGGGTCTTCCAGCAGGCCGATAACGt                         >  1:2377474/1‑49 (MQ=255)                       gATTTTCTTCACTACCGATTTCGGGTCTTCCAGCAGGCCGATAACGTTAt                      >  1:2453174/1‑50 (MQ=255)                               tcACTACCGATTTCGGGTCTTCCAGCAGGCCGATAACGTTATTGCGATTa              >  1:2694388/1‑50 (MQ=255)                                cACTACCGATTTCGGGTCTTCCAGCAGGCCGATAACGTTATTGCGATTAt             >  1:2604011/1‑50 (MQ=255)                                 aCTACCGATTTCGGGTCTTCCAGCAGGCCGATAACGTTATTGCGATTAt             <  1:2790649/49‑1 (MQ=255)                                 aCTACCGATTTCGGGTCTTCCAGCAGGCCGATAACGTTATTGCGATTATc            >  1:2120632/1‑50 (MQ=255)                                  cTACCGATTTCGGGTCTTCCAGCAGGCCGATAACGTTATTGCGATTATCg           <  1:1938759/50‑1 (MQ=255)                                     ccGATTTCGGGTCTTCCAGCAGGCCGATAACGTTATTGCGATTATCGt          <  1:2759855/48‑1 (MQ=255)                                     ccGATTTCGGGTCTTCCAGCAGGCCGATAACGTTATTGCGATTATCGt          <  1:558966/48‑1 (MQ=255)                                     ccGATTTCGGGTCTTCCAGCAGGCCGATAACGTTATTGCGATTATCGt          <  1:710754/48‑1 (MQ=255)                                       gATTTCGGGTCTTCCAGCAGGCCGATAACGTTATTGCGATTATCGTCAGa      <  1:1948338/50‑1 (MQ=255)                                        aTTTCGGGTCTTCCAGCAGGCCGATAACGTTATTGCGATTATCGTCAGAc     >  1:604452/1‑50 (MQ=255)                                        aTTTCGGGTCTTCCAGCAGGCCGATAACGTTATTGCGATTATCGTCAGAc     <  1:2032357/50‑1 (MQ=255)                                            cGGGTCTTCCAGCAGGCCGATAACGTTATTGCGATTATCGTCAGACTTg  >  1:2179603/1‑49 (MQ=255)                                            cGGGTCTTCCAGCAGGCCGATAACGTTATTGCGATTATCGTCAGACTTg  >  1:1472238/1‑49 (MQ=255)                                               |                                              CGGAGTCAGTGACCGCACGTTTGATTTTCTTCACTACCGATTTCGGATCTTCCAGCAGGCCGATAACGTTATTGCGATTATCGTCAGACTTG  >  NC_000913/3512957‑3513048

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑