Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	3,527,760	A→G	N431S (AAC→AGC)	igaA →	inner membrane protein ‑ inhibits the Rcs signaling pathway

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	3,527,760	0	A	G	100.0%	163.9 / NA	43	N431S (AAC→AGC)	igaA	inner membrane protein ‑ inhibits the Rcs signaling pathway
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base G (25/18);  total (25/18)

CCAATTCACCTTTTTTACCGTTTGACTGCTCGCAGATCATCTGGAACGACGCCCGCTCATTGCCATTACCAGAATCTGAACTGGTCAACA  >  NC_000913/3527715‑3527804                                              |                                             ccAATTCACCTTTTTTACCGTTTGACTGCTCGCAGATCATCTGGAgcgac                                          >  1:1908405/1‑50 (MQ=255)    aTTCACCTTTTTTACCGTTTGACTGCTCGCAGATCATCTGGAGCGACGcc                                       >  1:1069317/1‑50 (MQ=255)     ttCACCTTTTTTACCGTTTGACTGCTCGCAGATCATCTGGAGCGACGccc                                      <  1:1058091/50‑1 (MQ=255)      tCACCTTTTTTACCGTTTGACTGCTCGCAGATCATCTGGAGCGACGCCCg                                     >  1:2566880/1‑50 (MQ=255)        aCCTTTTTTACCGTTTGACTGCTCGCAGATCATCTGGAGCGACGCCCGc                                    <  1:1120487/49‑1 (MQ=255)        aCCTTTTTTACCGTTTGACTGCTCGCAGATCATCTGGAGCGACGCCCGc                                    <  1:1440092/49‑1 (MQ=255)        aCCTTTTTTACCGTTTGACTGCTCGCAGATCATCTGGAGCGACGCCCGCt                                   >  1:531896/1‑50 (MQ=255)        aCCTTTTTTACCGTTTGACTGCTCGCAGATCATCTGGAGCGACGCCCGCt                                   >  1:1116065/1‑50 (MQ=255)        aCCTTTTTTACCGTTTGACTGCTCGCAGATCATCTGGAGCGACGCCCGCt                                   <  1:2054535/50‑1 (MQ=255)        aCCTTTTTTACCGTTTGACTGCTCGCAGATCATCTGGAGCGACGCCCGCt                                   <  1:2503368/50‑1 (MQ=255)          cTTTTTTACCGTTTGACTGCTCGCAGATCATCTGGAGCGACGCCCGCTCa                                 >  1:1472374/1‑50 (MQ=255)          cTTTTTTACCGTTTGACTGCTCGCAGATCATCTGGAGCGACGCCCGCTCa                                 >  1:353139/1‑50 (MQ=255)              tttACCGTTTGACTGCTCGCAGATCATCTGGAGCGACGCCCGCTCATTGc                             >  1:1311417/1‑50 (MQ=255)                tACCGTTTGACTGCTCGCAGATCATCTGGAGCGACGCCCGCTCATTGcc                            <  1:2531553/49‑1 (MQ=255)                 aCCGTTTGACTGCTCGCAGATCATCTGGAGCGACGCCCGCTCATTGCCa                           <  1:2673290/49‑1 (MQ=255)                  ccGTTTGACTGCTCGCAGATCATCTGGAGCGACGCCCGCTCATTGCCAtt                         <  1:1406130/50‑1 (MQ=255)                   cGTTTGACTGCTCGCAGATCATCTGGAGCGACGCCCGCTCATTGCCATTa                        <  1:349092/50‑1 (MQ=255)                   cGTTTGACTGCTCGCAGATCATCTGGAGCGACGCCCGCTCATTGCCATTa                        >  1:1106305/1‑50 (MQ=255)                   cGTTTGACTGCTCGCAGATCATCTGGAGCGACGCCCGCTCATTGCCATTa                        <  1:1813922/50‑1 (MQ=255)                    gTTTGACTGCTCGCAGATCATCTGGAGCGACGCCCGCTCATTGCCATTAc                       >  1:1891214/1‑50 (MQ=255)                      ttGACTGCTCGCAGATCATCTGGAGCGACGCCCGCTCATTGCCATTAc                       >  1:1081313/1‑48 (MQ=255)                       tGACTGCTCGCAGATCATCTGGAGCGACGCCCGCTCATTGCCATTACCa                     <  1:2692737/49‑1 (MQ=255)                         aCTGCTCGCAGATCATCTGGAGCGACGCCCGCTCATTGCCATTACCAGaa                  >  1:702876/1‑50 (MQ=255)                          cTGCTCGCAGATCATCTGGAGCGACGCCCGCTCATTGCCATTACCAGAAt                 >  1:971194/1‑50 (MQ=255)                            gCTCGCAGATCATCTGGAGCGACGCCCGCTCATTGCCATTACCAGAATCt               >  1:1721063/1‑50 (MQ=255)                             cTCGCAGATCATCTGGAGCGACGCCCGCTCATTGCCATTACCAGAATCt               <  1:622075/49‑1 (MQ=255)                             cTCGCAGATCATCTGGAGCGACGCCCGCTCATTGCCATTACCAGAATCTg              <  1:1862071/50‑1 (MQ=255)                             cTCGCAGATCATCTGGAGCGACGCCCGCTCATTGCCATTACCAGAATCTg              <  1:856648/50‑1 (MQ=255)                              tCGCAGATCATCTGGAGCGACGCCCGCTCATTGCCATTACCAGAATCTg              >  1:2520235/1‑49 (MQ=255)                                gCAGATCATCTGGAGCGACGCCCGCTCATTGCCATTACCAGAATCTGa             <  1:1691540/48‑1 (MQ=255)                                gCAGATCATCTGGAGCGACGCCCGCTCATTGCCATTACCAGAATCTGa             <  1:1574261/48‑1 (MQ=255)                                gCAGATCATCTGGAGCGACGCCCGCTCATTGCCATTACCAGAATCTGAAc           >  1:1811271/1‑50 (MQ=255)                                gCAGATCATCTGGAGCGACGCCCGCTCATTGCCATTACCAGAATCTGAAc           >  1:714665/1‑50 (MQ=255)                                 cAGATCATCTGGAGCGACGCCCGCTCATTGCCATTACCAGAATCTGAACt          >  1:1027090/1‑50 (MQ=255)                                  aGATCATCTGGAGCGACGCCCGCTCATTGCCATTACCAGAATCTGAACTg         <  1:1889859/50‑1 (MQ=255)                                  aGATCATCTGGAGCGACGCCCGCTCATTGCCATTACCAGAATCTGAACTg         >  1:2120667/1‑50 (MQ=255)                                  aGATCATCTGGAGCGACGCCCGCTCATTGCCATTACCAGAATCTGAACTg         >  1:193127/1‑50 (MQ=255)                                   gATCATCTGGAGCGACGCCCGCTCATTGCCATTACCAGAATCTGAACTgg        >  1:1636720/1‑50 (MQ=255)                                     tcatcTGGAGCGACGCCCGCTCATTGCCATTACCAGAATCTGAACTGGt       >  1:156955/1‑49 (MQ=255)                                       atcTGGAGCGACGCCCGCTCATTGCCATTACCAGAATCTGAACTGGTcaa    >  1:1715774/1‑50 (MQ=255)                                       atcTGGAGCGACGCCCGCTCATTGCCATTACCAGAATCTGAACTGGTca     <  1:2375860/49‑1 (MQ=255)                                         cTGGAGCGACGCCCGCTCATTGCCATTACCAGAATCTGAACTGGTcaac   >  1:170331/1‑49 (MQ=255)                                          tGGAGCGACGCCCGCTCATTGCCATTACCAGAATCTGAACTGGTcaaca  >  1:2719926/1‑49 (MQ=255)                                           ggAGCGACGCCCGCTCATTGCCATTACCAGAATCTGAACTGGTcaaca  >  1:670714/1‑48 (MQ=255)                                              |                                             CCAATTCACCTTTTTTACCGTTTGACTGCTCGCAGATCATCTGGAACGACGCCCGCTCATTGCCATTACCAGAATCTGAACTGGTCAACA  >  NC_000913/3527715‑3527804

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 30 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑