Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	380,022	(G)10→9	intergenic (‑141/+47)	frmR ← / ← yaiO	DNA‑binding transcriptional repressor FrmR/outer membrane protein YaiO

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	380,013	0	G	.	100.0%	72.4 / NA	16	intergenic (‑132/+56)	frmR/yaiO	DNA‑binding transcriptional repressor FrmR/outer membrane protein YaiO
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base . (12/4);  total (12/4)

GAGAATAAAATTTATCCGGTGAATGTGGTCGGAAAACAAAGAGGAAAGGGGGGGGGGCTAATCGGCAGGGAAGGCCGCCCCGGATAGCGGGC  >  NC_000913/379966‑380057                                                |                                             gagaATAAAATTTATCCGGTGAATGTGGTCGGAAAACAAAGAGGAAAggg                                            <  1:1136894/50‑1 (MQ=255) gagaATAAAATTTATCCGGTGAATGTGGTCGGAAAACAAAGAGGAAAgg                                             <  1:2633279/49‑1 (MQ=255)  agaATAAAATTTATCCGGTGAATGTGGTCGGAAAACAAAGAGGAAAgggg                                           <  1:912009/50‑1 (MQ=255)    aaTAAAATTTATCCGGTGAATGTGGTCGGAAAACAAAGAGGAAAgggggg                                         >  1:706352/1‑50 (MQ=255)    aaTAAAATTTATCCGGTGAATGTGGTCGGAAAACAAAGAGGAAAgggggg                                         >  1:2601872/1‑50 (MQ=255)        aaaTTTATCCGGTGAATGTGGTCGGAAAACAAAGAGGAAAgggggggggc                                     >  1:340855/1‑49 (MQ=255)        aaaTTTATCCGGTGAATGTGGTCGGAAAACAAAGAGGAAAgggggggggc                                     <  1:2210507/50‑2 (MQ=255)         aaTTTATCCGGTGAATGTGGTCGGAAAACAAAGAGGAAAgggggggggc                                     >  1:1671567/1‑48 (MQ=255)         aaTTTATCCGGTGAATGTGGTCGGAAAACAAAGAGGAAAgggggggggc                                     >  1:2406716/1‑48 (MQ=255)            ttATCCGGTGAATGTGGTCGGAAAACAAAGAGGAAA‑GGGGGGGGGCTAAt                                >  1:544783/1‑50 (MQ=255)            ttATCCGGTGAATGTGGTCGGAAAACAAAGAGGAAA‑GGGGGGGGGCTAAt                                >  1:2054750/1‑50 (MQ=255)             tATCCGGTGAATGTGGTCGGAAAACAAAGAGGAAA‑GGGGGGGGGCTAAt                                <  1:1224540/49‑1 (MQ=255)                    tGAATGTGGTCGGAAAACAAAGAGGAAA‑GGGGGGGGGCTAATCGGCAgg                         >  1:943924/1‑49 (MQ=255)                     gAATGTGGTCGGAAAACAAAGAGGAAA‑GGGGGGGGGCTAATCGGCAggg                        >  1:214646/1‑49 (MQ=255)                     gAATGTGGTCGGAAAACAAAGAGGAAA‑GGGGGGGGGCTAATCGGCAggg                        >  1:1997062/1‑49 (MQ=255)                     gAATGTGGTCGGAAAACAAAGAGGAAA‑GGGGGGGGGCTAATCGGCAggg                        >  1:759526/1‑49 (MQ=255)                        tgtgGTCGGAAAACAAAGAGGAAA‑‑GGGGGGGGCTAATCGGCAGGGAAGgc                   >  1:2055353/1‑50 (MQ=255)                          tgGTCGGAAAACAAAGAGGAAA‑GGGGGGGGGCTAATCGGCAGGGAAGgcc                  <  1:372877/50‑1 (MQ=255)                          tgGTCGGAAAACAAAGAGGAAA‑GGGGGGGGGCTAATCGGCAGGGAAGgcc                  <  1:407702/50‑1 (MQ=255)                              cGGAAAACAAAGAGGAAA‑GGGGGGGGGCTAATCGGCAGGGAAGGCCGccc              >  1:1693341/1‑50 (MQ=255)                                  aaaCAAAGAGGAAA‑GGGGGGGGGCTAATCGGCAGGGAAGGCCGCCCCGGa          >  1:1576728/1‑50 (MQ=255)                                     cAAAGAGGAAA‑‑GGGGGGGGCTAATCGGCAGGGAAGGCCGCCCCGGATAgc      <  1:883516/50‑1 (MQ=255)                                        agagGAAA‑GGGGGGGGGCTAATCGGCAGGGAAGGCCGCCCCGGATAgcgg    >  1:2314867/1‑50 (MQ=255)                                         gagGAAA‑GGGGGGGGGCTAATCGGCAGGGAAGGCCGCCCCGGATAgcggg   >  1:523039/1‑50 (MQ=255)                                          agGAAA‑GGGGGGGGGCTAATCGGCAGGGAAGGCCGCCCCGGATAgcgggc  >  1:562030/1‑50 (MQ=255)                                                |                                             GAGAATAAAATTTATCCGGTGAATGTGGTCGGAAAACAAAGAGGAAAGGGGGGGGGGCTAATCGGCAGGGAAGGCCGCCCCGGATAGCGGGC  >  NC_000913/379966‑380057

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 16 ≤ ATCG/ATCG < 31 ≤ ATCG/ATCG < 35 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑